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RCB0796 : 1C3D3  update : 2018/07/17
CommentHuman embryonic pancreas-derived cell line. See RCB0794,0795,0797.
Comment from the depositor
Restriction (Category)b
Terms and conditionsA prior written permission of the approver(Depositor) is necessary for any kind of use including academic use and for-profit use.
Remarks
approver's address
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English
Address
Contact Us
cellbank.brc@riken.jp
RIKEN, BioResource Research Center
Cell Engineering Division
Dr.Cell Bank
Fax. +81-29-836-9130
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Ishikawa, Hiroshi
Originator Ishikawa, Hiroshi
Year of deposit 1992
Animal human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Unknown
Age at sampling 19 weeks embryo
Tissue embryo, pancreas
Classification other
Year of origin 1991
History Direct from originator
Memo_1 Mycoplasma was eliminated with MC-210.
Lifespan finite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives HamF10 + 5% FBS + 10% NBS + 2.5% HS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR (human) OK
Isozyme LD, NP
Chromosome mode 46-171(50) : /51(2),52(6),53(5),54(3),55(4),56(2)/
Images
deposit info
lot info
Reference information Reference 0
User's Publication 1


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Reference

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User's Publication
Shoji Y, Miyamoto M, Ishikawa K, Yoshioka T, Mishra R, Ichinokawa K, Matsumura Y, Itoh T, Shinohara T, Hirano S, Kondo S.  The CD40-CD154 interaction would correlate with proliferation and immune escape in pancreatic ductal adenocarcinoma.  J Surg Oncol  2011  103:230-8  PubMed ID: 21337550



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