Cell No. : Cell Name
RCB0782 : RC4
update : 2022/03/24
|
| Comment | Normal rabbit cornea cells. Usually transform spontaneously during serial culture. |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Watanabe, Masami
|
| Originator |
Watanabe, Masami
|
| Year of deposit |
1992
|
| Animal |
_rabbit
< Mammals
|
| Tissue |
cornea
|
| Disease name |
normal rabbit
|
| Classification |
normal
|
|
Lifespan |
infinite
|
| Morphology |
fibroblast-like
|
| Cellosaurus(Expasy) |
CVCL_6718
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
| Culture medium |
|
MEM + 10% FBS
|
| Antibiotics |
|
Free
|
|
Passage method |
|
0.25% Trypsin
|
Culture information
|
Passage cell No |
|
5 x 10 5 cells/100 mmdish
|
| SC frequency |
|
Subculture : 1-2 times/week, Medium Renewal : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma |
|
(-)
|
| Animal PCR |
|
OK
|
| Isozyme |
|
LD, NP
|
| Chromosome mode |
|
41-177(50) : /43(4),44(26),45(3),46(1),84(1),87(2)/
|
| Images |
deposit info | lot info |
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|
|
| Reference information |
Reference |
0
|
| User's Publication |
3
|
| User's Publication |
|
18153
Uchida K, Unuma K, Funakoshi T, Aki T, Uemura K.
Activation of Master Autophagy Regulator TFEB During Systemic LPS Administration in the Cornea
J Toxicol Pathol
2014
27(2):153-8
PubMed ID: 25352718
DOI: 10.1293/tox.2014-0004
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|
18154
Sumide T, Tsuchiya T.
Effects of multipurpose solutions (MPS) for hydrogel contact lenses on gap-junctional intercellular communication (GJIC) in rabbit corneal keratocytes
J Biomed Mater Res B Appl Biomater
2003
64(2):57-64
PubMed ID: 12516079
DOI: 10.1002/jbm.b.10510
|
|
18155
Terada S, Nishimura T, Sasaki M, Yamada H, Miki M.
Sericin, a protein derived from silkworms, accelerates the proliferation of several mammalian cell lines including a hybridoma
Cytotechnology
2002
40(1-3):3-12
PubMed ID: 19003099
DOI: 10.1023/A:1023993400608
|
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