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RCB0778 : CW-2  update : 2018/07/06
CommentColon carcinoma. CEA-positive, nude mouse-transplantable.
Comment from the depositor
Restriction (Category)a
Terms and conditionsBasically, there is no restriction regarding academic use.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Mitsuhashi, Maki
Originator Mitsuhashi, Maki
Year of deposit 1992
Animal human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Female
Age at sampling 55 years
Tissue colon
Classification cancer
Year of origin 1990
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR (human) OK
Virus (HIV) (-)
Isozyme LD, NP
Chromosome mode 44-92(50) : 44(1),45(5),46(42),47(1),92(1)
Reference information Reference 0
User's Publication 10


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Reference

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User's Publication
7334  Ito H, Hasegawa K, Hasegawa Y, Nishimaki T, Hosomichi K, Kimura S, Ohba M, Yao H, Onimaru M, Inoue I, Inoue H.  Silver Nanoscale Hexagonal Column Chips for Detecting Cell-free DNA and Circulating Nucleosomes in Cancer Patients.  Sci Rep  2015  5:10455  PubMed ID: 25994878
7414  Kaku Y, Tsuchiya A, Kanno T, Nishizaki T.  Irinotecan induces cell cycle arrest, but not apoptosis or necrosis, in Caco-2 and CW2 colorectal cancer cell lines.  Pharmacology  2015  95:154-9  PubMed ID: 25833236
7823  Matsuo T, Nishizuka SS, Ishida K, Iwaya T, Ikeda M, Wakabayashi G.  Analysis of the anti-tumor effect of cetuximab using protein kinetics and mouse xenograft models.  BMC Res Notes  2011  4:140  PubMed ID: 21554739
8475  Saito M, Yaguchi T, Yasuda Y, Nakano T, Nishizaki T.  Adenosine suppresses CW2 human colonic cancer growth by inducing apoptosis via A(1) adenosine receptors.  Cancer Lett.  2009  290:211-5  PubMed ID: 19822392
8943  Sun L, Fu BB, Liu DG.  Systemic delivery of full-length C/EBP beta/liposome complex suppresses growth of human colon cancer in nude mice.  Cell Res.  2005  15:770-6  PubMed ID: 16246266
9508  Kaku Y, Tsuchiya A, Shimizu T, Tanaka A, Nishizaki T.  HUHS1015 Suppresses Colonic Cancer Growth by Inducing Necrosis and Apoptosis in Association with Mitochondrial Damage.  Anticancer Res.  2016  36:39-48  PubMed ID: 26722026
9823  Novotny CJ, Pollari S, Park JH, Lemmon MA, Shen W, Shokat KM.  Overcoming resistance to HER2 inhibitors through state-specific kinase binding.  Nat. Chem. Biol.  2016  12:923-930  PubMed ID: 27595329
10398  Matsushita N, Yamamoto S, Inoue Y, Aruga A, Yamamoto M.  RT-qPCR analysis of the tumor antigens TOMM34 and RNF43 in samples extracted from paraffin-embedded specimens of colorectal cancer.  Oncol Lett  2017  14:2281-2287  PubMed ID: 28789449
10498  Inaguma S, Lasota J, Felisiak-Golabek A, Kowalik A, Wang Z, Zieba S, Kalisz J, Ikeda H, Miettinen M.  Histopathological and genotypic characterization of metastatic colorectal carcinoma with PD-L1 (CD274)-expression: Possible roles of tumour micro environmental factors for CD274 expression.  J Pathol Clin Res  2017  3:268-278  PubMed ID: 29085667
11364  Kimura T, Tsukada A, Fukutomi T, Ichida K, Ohtsuki S, Sakurai H.  Urate Transport via Paracellular Route across Epithelial Cells.  Biol. Pharm. Bull.  2019    PubMed ID: 30606989



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