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RCB0774 : EC-GI-10  update : 2018/07/06
CommentProduce hypercalcemia in nude mice. PTH-related protein, IL-1alpha producing.
Comment from the depositor
Terms and conditionsBasically, there is no restriction regarding academic use.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Sato, Kanji
Originator Sato, Kanji
Year of deposit 1992
Another name EC-GI
Animal human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Male
Age at sampling 65 years
Tissue esophagus, lymph node meta
Case history Esophageal carcinoma
Metastastatic ability Yes
Metastatsd tissue lympho node
Classification cancer
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives HamF12 + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR(human) OK
Virus (HIV) (-)
Isozyme LD, NP
Chromosome mode 62-134(50) : /67(6),68(5),69(5),70(4),71(1),72(6)/
Images
deposit info
lot info
Reference information Reference 4
User's Publication 6


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Reference
1581  Sato K, Satoh T, Shizume K, Yamakawa Y, Ono Y, Demura H, Akatsu T, Takahashi N, Suda T  Prolonged decrease of serum calcium concentration by murine gamma-interferon in hypercalcemic, human tumor (EC-GI)-bearing nude mice.  Cancer Res  1992  52:444-9  PubMed ID: 1728416
1997  Yasuda T, Banville D, Hendy, G N, Goltzman D  Characterization of the human parathyroid hormone-like peptide gene. Functional and evolutionary aspects.  J Biol Chem  1989  264:7720-5  PubMed ID: 2708388
1580  Sato K, Fujii Y, Kasono K, Tsushima T, Shizume K  Production of interleukin-1 alpha and a parathyroid hormone-like factor by a squamous cell carcinoma of the esophagus (EC-GI) derived from a patient with hypercalcemia.  J Clin Endocrinol Metab  1988  67:592-601  PubMed ID: 3261732
1579  Sato K, Kasono K, Ohba Y, Yashiro T, Fujii Y, Yoshida, M A, Tsushima T, Shizume K  Establishment of A Parathyroid Hormone-like Factor-producing Esophageal Carcinoma Cell Line (EC-GI)  Jpn J Cancer Res  1987  78:1044-1048  PubMed ID: 2824416

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User's Publication
11101  Sato N, Fujishima F, Nakamura Y, Aoyama Y, Onodera Y, Ozawa Y, Ito K, Ishida H, Kamei T, Watanabe M, Sasano H.  Myosin 5a regulates tumor migration and epithelial-mesenchymal transition in esophageal squamous cell carcinoma: utility as a prognostic factor.  Hum. Pathol.  2018  80:113-122  PubMed ID: 29898384
7382  Saito S, Morishima K, Ui T, Hoshino H, Matsubara D, Ishikawa S, Aburatani H, Fukayama M, Hosoya Y, Sata N, Lefor AK, Yasuda Y, Niki T.  The role of HGF/MET and FGF/FGFR in fibroblast-derived growth stimulation and lapatinib-resistance of esophageal squamous cell carcinoma.  BMC Cancer  2015  15:82  PubMed ID: 25884729
6410  Danjoh I, Sone H, Noda N, Iimura E, Nagayoshi M, Saijo K, Hiroyama T, Nakamura Y.  Is parainfluenza virus a threatening virus for human cancer cell lines?  Hum Cell  2009  22(3):81-4  PubMed ID: 19624309
3369  Shimada, Hideaki, Nakashima, Kazue, Ochiai, Takenori, Nabeya, Yoshihiro, Takiguchi, Masaki, Nomura, Fumio, Hiwasa, Takaki  Serological identification of tumor antigens of esophageal squamous cell carcinoma.  Int J Oncol  2005  26:77-86  PubMed ID: 15586227
3796  Fukami T, Fukuhara H, Kuramochi M, Maruyama T, Isogai K, Sakamoto M, Takamoto S, Murakami Y.  Promoter methylation of the TSLC1 gene in advanced lung tumors and various cancer cell lines.  Int J Cancer  2003  107(1):53-9  PubMed ID: 12925956
5948  Ikeguchi M, Kaibara N.  survivin messenger RNA expression is a good prognostic biomarker for oesophageal carcinoma.  Br J Cancer  2002  87(8):883-7  PubMed ID: 12373603



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