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Cell No. : Cell Name
RCB0774 : EC-GI-10  update : 2024/02/13
CommentProduce hypercalcemia in nude mice. PTH-related protein, IL-1alpha producing.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Sato, Kanji
Originator Sato, Kanji
Year of deposit 1992
Another name EC-GI
Animal _human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Male
Age at sampling 65 years
Tissue esophagus, lymph node meta
Disease name Esophageal carcinoma
Metastatic ability Yes
Metastatic tissue lympho node
Classification cancer
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_1187
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium HamF12 + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) (-)
Isozyme LD, NP
Chromosome mode 62-134(50) : /67(6),68(5),69(5),70(4),71(1),72(6)/
STR(human) OK
Images
deposit info
lot info
Reference information Reference 5
User's Publication 21


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Reference
1581  Sato K, Satoh T, Shizume K, Yamakawa Y, Ono Y, Demura H, Akatsu T, Takahashi N, Suda T  Prolonged decrease of serum calcium concentration by murine gamma-interferon in hypercalcemic, human tumor (EC-GI)-bearing nude mice.  Cancer Res  1992  52:444-9  PubMed ID: 1728416  
1997  Yasuda T, Banville D, Hendy, G N, Goltzman D  Characterization of the human parathyroid hormone-like peptide gene. Functional and evolutionary aspects.  J Biol Chem  1989  264:7720-5  PubMed ID: 2708388  
124  Sato K, Fujii Y, Kasono K, Ozawa M, Imamura H, Kanaji Y, Kurosawa H, Tsushima T, Shizume K.  Parathyroid hormone-related protein and interleukin-1 alpha synergistically stimulate bone resorption in vitro and increase the serum calcium concentration in mice in vivo  Endocrinology  1989  124(5):2172-8  PubMed ID: 2539970   DOI: 10.1210/endo-124-5-2172
1580  Sato K, Fujii Y, Kasono K, Tsushima T, Shizume K  Production of interleukin-1 alpha and a parathyroid hormone-like factor by a squamous cell carcinoma of the esophagus (EC-GI) derived from a patient with hypercalcemia.  J Clin Endocrinol Metab  1988  67:592-601  PubMed ID: 3261732   DOI: 10.1210/jcem-67-3-592
1579  Sato K, Kasono K, Ohba Y, Yashiro T, Fujii Y, Yoshida, M A, Tsushima T, Shizume K  Establishment of A Parathyroid Hormone-like Factor-producing Esophageal Carcinoma Cell Line (EC-GI)  Jpn J Cancer Res  1987  78:1044-1048  PubMed ID: 2824416  

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User's Publication
21175  Zhang X, Wang Y, Zhang X, Shen Y, Yang K, Ma Q, Qiao Y, Shi J, Wang Y, Xu L, Yang B, Ge G, Hu L, Kong X, Yang C, Chen Y, Ding J, Meng L.  Intact regulation of G1/S transition renders esophageal squamous cell carcinoma sensitive to PI3Kα inhibitors.  Signal Transduct Target Ther  2023  8(1):153  PubMed ID: 37041169   DOI: 10.1038/s41392-023-01359-x
11511  Behan FM, Iorio F, Picco G, Gonçalves E, Beaver CM, Migliardi G, Santos R, Rao Y, Sassi F, Pinnelli M, Ansari R, Harper S, Jackson DA, McRae R, Pooley R, Wilkinson P, van der Meer D, Dow D, Buser-Doepner C, Bertotti A, Trusolino L, Stronach EA, Saez-Rodriguez J, Yusa K, Garnett MJ.  Prioritization of cancer therapeutic targets using CRISPR-Cas9 screens.  Nature  2019    PubMed ID: 30971826   DOI: 10.1038/s41586-019-1103-9
11101  Sato N, Fujishima F, Nakamura Y, Aoyama Y, Onodera Y, Ozawa Y, Ito K, Ishida H, Kamei T, Watanabe M, Sasano H.  Myosin 5a regulates tumor migration and epithelial-mesenchymal transition in esophageal squamous cell carcinoma: utility as a prognostic factor.  Hum. Pathol.  2018  80:113-122  PubMed ID: 29898384   DOI: 10.1016/j.humpath.2018.06.002
12638  Nakayama A, Aoki S, Uchihashi K, Nishijima-Matsunobu A, Yamamoto M, Kakihara N, Iwakiri R, Fujimoto K, Toda S.  Interaction between Esophageal Squamous Cell Carcinoma and Adipose Tissue in Vitro  Am J Pathol  2016  186(5):1180-94  PubMed ID: 26952643   DOI: 10.1016/j.ajpath.2016.01.003
12875  Ueno N, Shimizu A, Kanai M, Iwaya Y, Ueda S, Nakayama J, Seo MK.  Enhanced Expression of Fibroblast Growth Factor Receptor 3 IIIc Promotes Human Esophageal Carcinoma Cell Proliferation  J Histochem Cytochem  2016  64(1):7-17  PubMed ID: 26487184   DOI: 10.1369/0022155415616161
7382  Saito S, Morishima K, Ui T, Hoshino H, Matsubara D, Ishikawa S, Aburatani H, Fukayama M, Hosoya Y, Sata N, Lefor AK, Yasuda Y, Niki T.  The role of HGF/MET and FGF/FGFR in fibroblast-derived growth stimulation and lapatinib-resistance of esophageal squamous cell carcinoma.  BMC Cancer  2015  15:82  PubMed ID: 25884729   DOI: 10.1186/s12885-015-1065-8
15741  Zuguchi M, Miki Y, Onodera Y, Fujishima F, Takeyama D, Okamoto H, Miyata G, Sato A, Satomi S, Sasano H.  Estrogen receptor α and β in esophageal squamous cell carcinoma  Cancer Sci  2012  103(7):1348-55  PubMed ID: 22463081   DOI: 10.1111/j.1349-7006.2012.02288.x
6410  Danjoh I, Sone H, Noda N, Iimura E, Nagayoshi M, Saijo K, Hiroyama T, Nakamura Y.  Is parainfluenza virus a threatening virus for human cancer cell lines?  Hum Cell  2009  22(3):81-4  PubMed ID: 19624309   DOI: 10.1111/j.1749-0774.2009.00071.x
3369  Shimada, Hideaki, Nakashima, Kazue, Ochiai, Takenori, Nabeya, Yoshihiro, Takiguchi, Masaki, Nomura, Fumio, Hiwasa, Takaki  Serological identification of tumor antigens of esophageal squamous cell carcinoma.  Int J Oncol  2005  26:77-86  PubMed ID: 15586227  
18636  Nakashima K, Shimada H, Ochiai T, Kuboshima M, Kuroiwa N, Okazumi S, Matsubara H, Nomura F, Takiguchi M, Hiwasa T.  Serological identification of TROP2 by recombinant cDNA expression cloning using sera of patients with esophageal squamous cell carcinoma  Int J Cancer  2004  112(6):1029-35  PubMed ID: 15386348   DOI: 10.1002/ijc.20517
12151  Nakajima Y, Miyake S, Tanaka K, Ogiya K, Toukairin Y, Kawada K, Nishikage T, Nagai K, Kawano T.  The expressions of p21 and pRB may be good indicators for the sensitivity of esophageal squamous cell cancers to CPT-11: Cell proliferation activity correlates with the effect of CPT-11  Cancer Sci  2004  95(5):464-8  PubMed ID: 15132777   DOI: 10.1111/j.1349-7006.2004.tb03233.x
4700  Ikeguchi M, Yamaguchi K, Kaibara N.  Survivin gene expression positively correlates with proliferative activity of cancer cells in esophageal cancer  Tumour Biol  2003  24(1):40-5  PubMed ID: 12743425   DOI: 10.1159/000070659
3796  Fukami T, Fukuhara H, Kuramochi M, Maruyama T, Isogai K, Sakamoto M, Takamoto S, Murakami Y.  Promoter methylation of the TSLC1 gene in advanced lung tumors and various cancer cell lines.  Int J Cancer  2003  107(1):53-9  PubMed ID: 12925956   DOI: 10.1002/ijc.11348
13721  Ikeguchi M, Fukuda K, Yamaguchi K, Kondo A, Tsujitani S, Kaibara N.  Quantitative analysis of heparanase gene expression in esophageal squamous cell carcinoma  Ann Surg Oncol  2003  10(3):297-304  PubMed ID: 12679316   DOI: 10.1245/aso.2003.05.022
13750  Ito M, Amizuka N, Tanaka S, Funatsu-Ozawa Y, Kenmotsu S, Oda K, Nakajima T, Ozawa H.  Ultrastructural and cytobiological studies on possible interactions between PTHrP-secreting tumor cells, stromal cells, and bone cells  J Bone Miner Metab  2003  21(6):353-62  PubMed ID: 14586791   DOI: 10.1007/s00774-003-0429-3
16678  Okamoto Y, Ozaki T, Miyazaki K, Aoyama M, Miyazaki M, Nakagawara A.  UbcH10 is the cancer-related E2 ubiquitin-conjugating enzyme  Cancer Res  2003  63(14):4167-73  PubMed ID: 12874022  
4070  Sugiura T, Nakane S, Kishimoto S, Waku K, Yoshioka Y, Tokumura A.  Lysophosphatidic acid, a growth factor-like lipid, in the saliva  J Lipid Res  2002  43(12):2049-55  PubMed ID: 12454265   DOI: 10.1194/jlr.m200242-jlr200
5948  Ikeguchi M, Kaibara N.  survivin messenger RNA expression is a good prognostic biomarker for oesophageal carcinoma.  Br J Cancer  2002  87(8):883-7  PubMed ID: 12373603   DOI: 10.1038/sj.bjc.6600546
18756  Shimada H, Shimizu T, Ochiai T, Liu TL, Sashiyama H, Nakamura A, Matsubara H, Gunji Y, Kobayashi S, Tagawa M, Sakiyama S, Hiwasa T.  Preclinical study of adenoviral p53 gene therapy for esophageal cancer  Surg Today  2001  31(7):597-604  PubMed ID: 11495154   DOI: 10.1007/s005950170093
12275  Nakajima Y, Miyake S, Nagai K, Kawano T, Iwai T.  CPT-11 may provide therapeutic efficacy for esophageal squamous cell cancer and the effects correlate with the level of DNA topoisomerase I protein  Jpn J Cancer Res  2001  92(12):1335-41  PubMed ID: 11749700   DOI: 10.1111/j.1349-7006.2001.tb02158.x
13674  Miyauchi M, Shimada H, Kadomatsu K, Muramatsu T, Matsubara S, Ikematsu S, Takenaga K, Asano T, Ochiai T, Sakiyama S, Tagawa M.  Frequent expression of midkine gene in esophageal cancer suggests a potential usage of its promoter for suicide gene therapy  Jpn J Cancer Res  1999  90(4):469-75  PubMed ID: 10363587   DOI: 10.1111/j.1349-7006.1999.tb00771.x


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