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細胞番号 : 細胞名
RCB0763 : GI-1  update : 2022/08/24
細胞特性(Comment:英)Human glioma separated from gliosarcoma.
細胞特性(日)ヒトgliosarcomaから分離されたglioma細胞株。
細胞特性(寄託者記述:英)
細胞特性(寄託者記述:日)
使用条件(英)There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
使用条件(日)基礎的研究につきましては、使用制限はありません。商業利用や特許取得を目的とする場合は、事前に必ず理研細胞バンクに連絡をすること。
備考(英)
備考(日)
提供申込書類(英) Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
提供申込書類(日) 依頼書C-0001.pdf   同意書(非営利学術目的)C-0003.pdf   同意書(営利目的)C-0003p.pdf  
提供同意書は、使用機関の種類や目的に応じて、非営利学術目的 (C-XXXX) と営利目的 (C-XXXXp) の2種類があります。該当する提供同意書をご使用ください(詳細)。特許等の取得及び商業利用等は事前に必ず cellbank.brc@riken.jp までご連絡ください。
提供手数料 手数料とお支払いについてはこちらをご覧ください。
細胞基本情報 寄託者 Nakatu, Syouji
樹立者 Iwasaki, Kouji
寄託日 1992
動物種 _human < Mammals
属名 Homo
種名 sapiens
人種 Japanese
性別 Male
61 years
採取組織 brain
病名 gliosarcoma
転移 No
細胞分類 cancer
メモ_1 Mycoplasma was eliminated with MC-210 by the depositor.
細胞寿命 infinite
細胞形態 fibroblast-like
Cellosaurus(Expasy) CVCL_1231
細胞培養・検査情報
寄託時情報
ロット情報
培地・試薬情報 培地・試薬一覧はこちらをご覧ください。
培養形態 Adherent cells
培地 DMEM (low glucose) + 10% FBS
抗生物質 Free
継代方法 0.25% Trypsin
継代密度 1 : 8 split
継代・培地交換頻度 Subculture : 2 times/week
培養最適温度 37 ℃
二酸化炭素濃度 5 %
凍結培地 Medium + 10% DMSO
凍結方法 Slow freezing
マイコプラズマ (-)
ウィルス(HIV) (-)
アイソザイム検査 LD, NP
染色体検査 81-194(50) : /93(4),94(4),95(3),96(4),97(4),98(6)/
個体識別検査 OK
画像情報
寄託時情報
ロット情報
文献情報 Reference(英) 7件
Reference(日) 1件
利用者成果(英) 15件
利用者成果(日) 0件

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Reference(英)
1587  Iwasaki K, Kikuchi H, Miyatake S, Kondo S, Oda Y  Establishment of a new cell line derived from a human gliosarcoma.  Neurosurgery  1992  30:228-35  PubMed ID: 1545891   DOI: 10.1227/00006123-199202000-00013
274  Kondo S, Miyatake S, Kikuchi H, Oda Y, Iwasaki K, Ohyama K, Namba Y.  Mechanism of interferon gamma-induced protection of human gliosarcoma cells from lymphokine-activated killer lysis: division of lymphokine-activated killer cells into natural killer- and T-like cells  Neurosurgery  1992  31(3):534-40  PubMed ID: 1407434   DOI: 10.1227/00006123-199209000-00016
276  Kondo S, Miyatake S, Iwasaki K, Oda Y, Kikuchi H, Zu Y, Shamoto M, Namba Y.  Human glioma-specific antigens detected by monoclonal antibodies  Neurosurgery  1992  30(4):506-11  PubMed ID: 1584348   DOI: 10.1227/00006123-199204000-00006
4812  Miyatake S, Kondou S, Aoki T, Iwasaki K, Ohyama K, Ootsuka S, Oda Y, Kikuchi H.  Immunomodulatory effects of interferons on target human gliosarcoma cells in the tumor-specific CTL- and LAK-mediated cytolysis]  No Shinkei Geka  1991  19(11):1053-9  PubMed ID: 1762655  
4811  Miyatake S, Kikuchi H, Oda Y, Nishioka T, Takahashi J, Kondoh S, Matsumoto M, Yamasaki T, Iwasaki K, Aoki T, et al.  Decreased susceptibility of lined human gliosarcoma cells to lymphokine-activated killer cell cytolysis by gamma-interferon treatment.  Cancer Res  1990  50(3):596-600  PubMed ID: 2137025  
275  Iwasaki K, Kikuchi H, Miyatake S, Oda Y, Yamashita J, Yamasaki T.  Clonal analysis in the ultrastructure of cell-to-cell interaction between a human glioma cell line and autologous tumor-specific cytotoxic T lymphocytes  Cell Immunol  1990  126(1):164-75  PubMed ID: 2302738   DOI: 10.1016/0008-8749(90)90309-f
4810  Miyatake S, Kikuchi H, Iwasaki K, Yamashita J, Li ZY, Namba Y, Hanaoka M.  Specific cytotoxic activity of T lymphocyte clones derived from a patient with gliosarcoma.  J Neurosurg  1988  69(5):751-9  PubMed ID: 3263476   DOI: 10.3171/jns.1988.69.5.0751

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Reference(日)
1689  近藤精二, 宮武伸一, 菊池晴彦, 織田祥史, 岩崎孝一, 青木友和, 大山憲治, 難波雄二郎  IFN-α処理による脳腫瘍細胞の, LAK 細胞に対する感受性の低下    1991     

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利用者成果(英)
16701  Chinen T, Yamazaki K, Hashimoto K, Fujii K, Watanabe K, Takeda Y, Yamamoto S, Nozaki Y, Tsuchiya Y, Takao D, Kitagawa D.  Centriole and PCM cooperatively recruit CEP192 to spindle poles to promote bipolar spindle assembly  J Cell Biol  2021  220(2):e202006085  PubMed ID: 33443571   DOI: 10.1083/jcb.202006085
11920  Takumi Chinen , Shohei Yamamoto, Yutaka Takeda , Koki Watanabe, Kanako Kuroki , Kaho Hashimoto , Daisuke Takao , Daiju Kitagawa  NuMA assemblies organize microtubule asters to establish spindle bipolarity in acentrosomal human cells  EMBO J  2020    PubMed ID: 31782546   DOI: 10.15252/embj.2019102378
11511  Behan FM, Iorio F, Picco G, Gonçalves E, Beaver CM, Migliardi G, Santos R, Rao Y, Sassi F, Pinnelli M, Ansari R, Harper S, Jackson DA, McRae R, Pooley R, Wilkinson P, van der Meer D, Dow D, Buser-Doepner C, Bertotti A, Trusolino L, Stronach EA, Saez-Rodriguez J, Yusa K, Garnett MJ.  Prioritization of cancer therapeutic targets using CRISPR-Cas9 screens.  Nature  2019    PubMed ID: 30971826   DOI: 10.1038/s41586-019-1103-9
12337  Agarwal S, Mohamed MS, Mizuki T, Maekawa T, Sakthi Kumar D.  Chlorotoxin modified morusin-PLGA nanoparticles for targeted glioblastoma therapy  J Mater Chem B  2019  7(39):5896-5919  PubMed ID: 31423502   DOI: 10.1039/c9tb01131e
9629  Sakai S, Maruyama H, Kimura T, Tajiri K, Honda J, Homma S, Aonuma K, Miyauchi T.  Antagonists to endothelin receptor type B promote apoptosis in human pulmonary arterial smooth muscle cells.  Life Sci.  2016  159:116-120  PubMed ID: 27021787   DOI: 10.1016/j.lfs.2016.03.044
15425  Sreejith Raveendran, Neha Chauhan, Vivekanandan Palaninathan, Yutaka Nagaoka, Yasuhiko Yoshida, Toru Maekawa, D. Sakthi Kumar  Extremophilic Polysaccharide for Biosynthesis and Passivation of Gold Nanoparticles and Photothermal Ablation of Cancer Cells  Particle & Particle Systems Characterization  2015  32:54-64    DOI: 10.1002/ppsc.201400081
16168  Ravindran Girija Aswathy, Balasubramanian Sivakumar, Dhandayudhapani Brahatheeshwaran, Tomofumi Ukai, Yasuhiko Yoshida, Toru Maekawa, Sakthi D. Kumar  Biocompatible Fluorescent Jelly Quantum Dots for Bioimaging  Materials Express  2011  1:291-298    DOI: 10.1166/mex.2011.1045
14417  Sano M, Genkai N, Yajima N, Tsuchiya N, Homma J, Tanaka R, Miki T, Yamanaka R.  Expression level of ECT2 proto-oncogene correlates with prognosis in glioma patients  Oncol Rep  2006  16(5):1093-8  PubMed ID: 17016598  
14418  Homma J, Yamanaka R, Yajima N, Tsuchiya N, Genkai N, Sano M, Tanaka R.  Increased expression of CCAAT/enhancer binding protein beta correlates with prognosis in glioma patients  Oncol Rep  2006  15(3):595-601  PubMed ID: 16465418  
2958  Awata T, Kurihara S, Takata N, Neda T, Iizuka H, Ohkubo T, Osaki M, Watanabe M, Nakashima Y, Inukai K, Inoue I, Kawasaki I, Mori K, Yoneya S, Katayama S  Functional VEGF C-634G polymorphism is associated with development of diabetic macular edema and correlated with macular retinal thickness in type 2 diabetes.  Biochem Biophys Res Commun  2005  333:679-85  PubMed ID: 15963467   DOI: 10.1016/j.bbrc.2005.05.167
15603  Guleng B, Tateishi K, Ohta M, Asaoka Y, Jazag A, Lin LJ, Tanaka Y, Tada M, Seto M, Kanai F, Kawabe T, Omata M.  Identification of an epigenetically silenced gene, RFX1, in human glioma cells using restriction landmark genomic scanning  Oncogene  2004  23(47):7772-9  PubMed ID: 15334059   DOI: 10.1038/sj.onc.1208058
2959  Lambrechts, Diether, Storkebaum, Erik, Morimoto, Masafumi, Del-Favero, Jurgen, Desmet, Frederik, Marklund, Stefan L, Wyns, Sabine, Thijs, Vincent, Andersson, Jorgen, van Marion, Ingrid, Al-Chalabi, Ammar, Bornes, Stephanie, Musson, Rhiannon, Hansen, Valerie, Beckman, Lars, Adolfsson, Rolf, Pall, Hardev Singh, Prats, Herve, Vermeire, Severine, Rutgeerts, Paul, Katayama, Shigehiro, Awata, Takuya, Leigh, Nigel, Lang-Lazdunski, Loic, Dewerchin, Mieke, Shaw, Christopher, Moons, Lieve, Vlietinck, Robert, Morrison, Karen E, Robberecht, Wim, Van Broeckhoven, Christine, Collen, Desire, Andersen, Peter M, Carmeliet, Peter  VEGF is a modifier of amyotrophic lateral sclerosis in mice and humans and protects motoneurons against ischemic death.  Nat Genet  2003  34:383-94  PubMed ID: 12847526   DOI: 10.1038/ng1211
2550  Tamura, H O, Taniguchi K, Hayashi E, Hiyoshi Y, Nagai F  Expression profiling of sulfotransferases in human cell lines derived from extra-hepatic tissues.  Biol Pharm Bull  2001  24:1258-62  PubMed ID: 11725959   DOI: 10.1248/bpb.24.1258
16388  S. Yoshida, K. Fukino, H. Harada, H. Nagai, I. Imoto, J. Inazawa, H. Takahashi, A. Teramoto, M. Emi  The c-Jun NH2-terminal kinase3 (JNK3) gene: genomic structure, chromosomal assignment, and loss of expression in brain tumors  Journal of Human Genetics  2001  42:182-187  PubMed ID: 11322657   DOI: 10.1007/s100380170086
4988  Kuramoto T.  Detection of MAGE-1 tumor antigen in brain tumor.  Kurume Med J  1997  44(1):43-51  PubMed ID: 9154761   DOI: 10.2739/kurumemedj.44.43

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