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Cell No. : Cell Name
RCB0760 : MT-8  update : 2022/03/23
CommentRat cell line derived from malignant fibrous histiocytoma. Characteristics of histiocytes are lacking. The tumor formed by this cell line consists of immature cells.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Yamate, Jyoji
Originator Yamate, Jyoji
Year of deposit 1992
Original cell MT-P
Animal _rat < Mammals
Strain name F344/DuCrj
Genus Rattus
Species norvegicus
Gender Male
Age at sampling 15 months
Tissue subcutis of the head
Disease name Malignant fibrous histiocytoma
Metastatic ability No
Classification cancer
Year of origin 1986
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_5337
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 13
User's Publication 0


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Reference
17473  Nishina H, Katou-Ichikawa C, Kuramochi M, Izawa T, Kuwamura M, Yamate J.  The localization and distribution of cells labeled by a somatic stem cell-recognizing antibody (A3) in rat colon development; possible presence of a new cell type forming the intestinal stem cell niche  J Toxicol Pathol  2019  32(1):37-48  PubMed ID: 30739994   DOI: 10.1293/tox.2018-0037
3495  Takashi Kotera 1 , Chisa Katou-Ichikawa, Anusha Hemamali Tennakoon, Miyuu Tanaka, Natsuki Tanaka, Takeshi Izawa, Mitsuru Kuwamura, Jyoji Yamate   Rat malignant fibrous histiocytoma (MFH)-derived cloned cell lines (MT-8 and MT-9) show different differentiation in mesenchymal stem cell lineage  Eep Toxicol Pathol  2015  67(10):499-507  PubMed ID: 26208870   DOI: 10.1016/j.etp.2015.07.004
3494  Jyoji Yamate, Keiko Ogata, Takahiro Yuasa, Mitsuru Kuwamura, Shigeo Takenaka, Daijirou Kumagai, Kazuyuki Itoh, Jonathan LaMarre  Adipogenic, osteogenic and myofibrogenic differentiations of a rat malignant fibrous histiocytoma (MFH)-derived cell line, and a relationship of MFH cells with embryonal mesenchymal, perivascular and bone marrow stem cells  Eur J Cancer  2007  43(18):2747-56  PubMed ID: 18036812   DOI: 10.1016/j.ejca.2007.10.021
3418  J Yamate, Y Yokoyama, D Kumagi, Y Tsukamoto, M Kuwamura, T Kotani, S Sakuma  Tumour lines from a spontaneous rat endometrial stromal sarcoma, showing dendritic cell-like and myofibroblastic cell-like phenotypes  J Comp Pathol   2004  131(1):38-51  PubMed ID: 15144798   DOI: 10.1016/j.jcpa.2004.01.003
2786  Tsunenari I, Yamate J, Iwaki M, Kuwamura M, Kotani T, Sakuma S  Angioarchitecture of tumors induced by two different cloned cell lines established from a transplantable rat malignant fibrous histiocytoma.  Microsc Microanal  2003  9:532-41  PubMed ID: 14750988   DOI: 10.1017/S1431927603030289
2788  Kumagai D, Yamate J, Tajima T, Tsukamoto Y, Yasui H, Kuwamura M, Kotani T, Sakuma S  Distribution of cells labelled by a monoclonal antibody (A3) against a cloned cell line derived from a rat malignant fibrous histiocytoma  J Comp Pathol  2001  123:77-87  PubMed ID: 11032659   DOI: 10.1053/jcpa.2000.0389
2787  Kumagai D, Yamate J, Tajima T, Kuwamura M, Kotani T, Sakuma S  Malignant fibrous histiocytoma (MFH) cells and macrophages/histiocytes have a common antigen recognized by a monoclonal antibody risen against a rat MFH-derived cloned cell line.  J Vet Med Sci  2001  63:333-5  PubMed ID: 11307938   DOI: 10.1292/jvms.63.333
2789  Tsunenari I, Yamate J, Sharma KD, Kawachi M, Sakuma S  Expressions of vascular endothelial growth factor and basic fibroblast growth factor in tumors induced by two different cloned cell lines established from transplantable rat malignant fibrous histiocytoma.  J Vet Med Sci  2000  62:699-705  PubMed ID: 10945286   DOI: 10.1292/jvms.62.699
2792  Yamate J, Tajima M, Shibuya K, Kuwamura M, Kotani T, Sakuma S, Takeya M  Heterogeneity in the origin and immunophenotypes of "histiocytic" cells in transplantable rat malignant fibrous histiocytoma.  J Vet Med Sci.  1996  58:603-609  PubMed ID: 8844594   DOI: 10.1292/jvms.58.603
2790  Yamate J, Tajima M, Shibuya K, Kuwamura M, Kotani T, Sakuma S  Phenotypic changes in lipopolysaccharide-treated cloned cells derived from transplantable rat malignant fibrous histiocytoma.  J Vet Med Sci  1996  58:1017-20  PubMed ID: 8916004   DOI: 10.1292/jvms.58.10_1017
2791  Yamate J, Tajima M, Shibuya K, Kuwamura M, Kotani T, Sakuma S  Phenotypic modulation in cisplatin-resistant cloned cells derived from transplantable rat malignant fibrous histiocytoma.  Pathol Int  1996  46:557-67  PubMed ID: 8893224   DOI: 10.1111/j.1440-1827.1996.tb03654.x
2793  Yamate J, Tajima M, Shibuya K, Saitoh T  Enhancement of lipid phagocytosis by cloned cells derived from rat malignant fibrous histiocytoma under hyperlipemic conditions.  Pathol Int  1994  44:735-44  PubMed ID: 7834074   DOI: 10.1111/j.1440-1827.1994.tb02920.x
1671  Yamate J, Tajima M, Togo M, Shibuya K, Ihara M, Kudow S  Heterogeneity of Cloned Cell Lines Established from a Transplantable Rat Malignamnt Fibrous Histiocytoma  Jpn J Cancer Res  1991  82:298-307  PubMed ID: 1850724   DOI: 10.1111/j.1349-7006.1991.tb01846.x

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