Cell No. : Cell Name
RCB0710 : HyLDH·YK-2
update : 2024/01/17
|
| Comment | Anti-rabbit muscle lactate dehydrogenase (LDH) mAb (IgG2b). Possible to culture in serum-free medium |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Shirahata, Sanetaka
|
| Originator |
Kichise, Yoshikatsu
|
| Year of deposit |
1991
|
| Animal |
_mouse x mouse
< Hybrid
|
| Tissue |
hybridoma
|
| Classification |
hybrid cells
|
|
Lifespan |
infinite
|
| Morphology |
lymphocyte-like
|
| Cellosaurus(Expasy) |
CVCL_L553
|
|
hybridoma
|
Fusion_cell_1 strain |
mouse, BALB/c
|
| Fusion_cell_2 name |
P3-X63Ag8.653
|
| Fusion_cell_2 strain |
mouse
|
|
Fusion method |
PEG, HAT selection
|
|
Antibody name |
LDH・YK-2
|
| Subclass |
IgG2b
|
| Immunogen |
Rabbit muscle LDH
|
|
Antigen_name |
Rabbit muscle LDH
|
| Antigen_m.w. |
140kDa
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Suspension cells
|
| Culture medium |
|
E-RDF + 5% FBS
|
| Culture method |
|
|
| Antibiotics |
|
Free
|
|
Passage method |
|
dilution
|
Culture information
|
Passage ratio |
|
1 : 10 split
|
| SC frequency |
|
Subculture : 3 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma |
|
(-)
|
| Antibody subclass |
|
IgG2b
|
| Chromosome mode |
|
66-107(50) : /84(6),85(4),86(6),87(7),88(6),89(3)/
|
| Reference information |
Reference |
0
|
| User's Publication |
0
|
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