細胞番号 : 細胞名 RCB0701 : 143B/TK^(-)neo^(R)  update : 2023/06/30
細胞特性(Comment:英)			v-Ki-ras transformant TE85 cell line. HAT sensitive, neo resistant.
細胞特性(日)			TE85をv-Ki-rasでtransformして得られた株。ヒト不死化細胞のC type に属する。
細胞特性(寄託者記述:英)
細胞特性(寄託者記述:日)
使用条件(英)			There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
使用条件(日)			基礎的研究につきましては、使用制限はありません。商業利用や特許取得を目的とする場合は、事前に必ず理研細胞バンクに連絡をすること。
備考(英)
備考(日)
提供申込書類(英)			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
提供申込書類(日)			依頼書C-0001.pdf   同意書(非営利学術目的)C-0003.pdf   同意書(営利目的)C-0003p.pdf
			提供同意書は、使用機関の種類や目的に応じて、非営利学術目的 (C-XXXX) と営利目的 (C-XXXXp) の2種類があります。該当する提供同意書をご使用ください（詳細）。特許等の取得及び商業利用等は事前に必ず cellbank.brc@riken.jp までご連絡ください。
提供手数料			手数料とお支払いについてはこちらをご覧ください。
細胞基本情報		寄託者	Sugimoto, Yoshikazu
		樹立者	Croce, C.
		寄託日	1991
		動物種	_human < Mammals
		属名	Homo
		種名	sapiens
		性別	Unknown
		採取組織	osteosarcoma
		細胞分類	transformed
		遺伝子改変	recombinant
		外来遺伝子	v-Ki-ras
		入手歴	Dr. Perira-Smith M. Olivia
		細胞寿命	infinite
		細胞形態	fibroblast-like
		Cellosaurus(Expasy)	CVCL_9W36
細胞培養・検査情報			寄託時情報	ロット情報
	培地・試薬情報		培地・試薬一覧はこちらをご覧ください。
		培養形態		Adherent cells
		培地		MEM + 10% FBS
		抗生物質		Free
		継代方法		0.25% Trypsin
		継代密度		1 : 8 split
		継代・培地交換頻度		Subculture : 2 times/week
		培養最適温度		37 ℃
		二酸化炭素濃度		5 %
		凍結培地		Medium + 10% DMSO
		凍結方法		Slow freezing
		マイコプラズマ		(-)
		アイソザイム検査		LD, NP
		染色体検査		46-125(50) : /48(11),49(13),50(10),51(4),53(2)/
		個体識別検査		OK
画像情報			寄託時情報	ロット情報
文献情報		Reference(英)	1件
		Reference(日)	0件
		利用者成果(英)	20件
		利用者成果(日)	0件

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Reference(英)

1531  Pereira-Smith, O M, Smith, J R  Genetic analysis of indefinite division in human cells: identification of four complementation groups.  Proc Natl Acad Sci U S A  1988  85:6042-6  PubMed ID: 3413074   DOI: 10.1073/pnas.85.16.6042

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Reference(日)

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利用者成果(英)

17824  Asanuma K, Nakamura T, Nakamura K, Hagi T, Okamoto T, Kita K, Matsuyama Y, Yoshida K, Asanuma Y, Sudo A.  Compound Library Screening for Synergistic Drug Combinations: mTOR Inhibitor and Proteasome Inhibitor Effective Against Osteosarcoma Cells  Anticancer Res  2022  42(9):4319-4328  PubMed ID: 36039444   DOI: 10.21873/anticanres.15932

16215  Manami Suzuki-Karasaki, Takashi Ando, Yushi Ochiai, Kenta Kawahara, Miki Suzuki-Karasaki, Hideki Nakayama, Yoshihiro Suzuki-Karasaki  Air Plasma-Activated Medium Evokes a Death-Associated Perinuclear Mitochondrial Clustering  Int J Mol Sci  2022  23(3):1124  PubMed ID: 35163042   DOI: 10.3390/ijms23031124

16415  Kuriyama S, Tanaka G, Takagane K, Itoh G, Tanaka M.  Pigment Epithelium Derived Factor Is Involved in the Late Phase of Osteosarcoma Metastasis by Increasing Extravasation and Cell-Cell Adhesion  Front Oncol  2022  12:818182  PubMed ID: 35174090   DOI: 10.3389/fonc.2022.818182

20751  Wada KI, Hosokawa K, Ito Y, Maeda M, Harada Y, Yonemitsu Y.  Generation of transmitochondrial cybrids using a microfluidic device.  Exp Cell Res  2022  418(1):113233  PubMed ID: 35659971   DOI: 10.1016/j.yexcr.2022.113233

16389  Ideta H, Yoshida K, Okamoto M, Sasaki J, Kito M, Aoki K, Yoshimura Y, Suzuki S, Tanaka A, Takazawa A, Haniu H, Uemura T, Takizawa T, Sobajima A, Kamanaka T, Takahashi J, Kato H, Saito N.  Antitumor Effect of Sclerostin against Osteosarcoma  Cancers (Basel)  2021  13(23):6015  PubMed ID: 34885123   DOI: 10.3390/cancers13236015

16865  Ando T, Suzuki-Karasaki M, Suzuki-Karasaki M, Ichikawa J, Ochiai T, Yoshida Y, Haro H, Suzuki-Karasaki Y.  Combined Anticancer Effect of Plasma-Activated Infusion and Salinomycin by Targeting Autophagy and Mitochondrial Morphology  Front Oncol  2021  11:593127  PubMed ID: 34150606   DOI: 10.3389/fonc.2021.593127

11888  Yoshida K, Okamoto M, Sasaki J, Kuroda C, Ishida H, Ueda K, Ideta H, Kamanaka T, Sobajima A, Takizawa T, Tanaka M, Aoki K, Uemura T, Kato H, Haniu H, Saito N.  Anti-PD-1 antibody decreases tumour-infiltrating regulatory T cells.  BMC Cancer  2020  20(1):25.  PubMed ID: 31914969   DOI: 10.1186/s12885-019-6499-y

11888  Yoshida K, Okamoto M, Sasaki J, Kuroda C, Ishida H, Ueda K, Ideta H, Kamanaka T, Sobajima A, Takizawa T, Tanaka M, Aoki K, Uemura T, Kato H, Haniu H, Saito N.  Anti-PD-1 antibody decreases tumour-infiltrating regulatory T cells.  BMC Cancer  2020  20(1):25.  PubMed ID: 31914969   DOI: 10.1186/s12885-019-6499-y

13141  Oka N, Komuro A, Amano H, Dash S, Honda M, Ota K, Nishimura S, Ueda T, Akagi M, Okada H.  Ascorbate sensitizes human osteosarcoma cells to the cytostatic effects of cisplatin.  Pharmacol Res Perspect  2020    PubMed ID: 32725721   DOI: 10.1002/prp2.632

13306  Ando T, Ichikawa J, Fujimaki T, Taniguchi N, Takayama Y, Haro H.  Gemcitabine and Rapamycin Exhibit Additive Effect against Osteosarcoma by Targeting Autophagy and Apoptosis.  Cancers (Basel)  2020    PubMed ID: 33114161   DOI: 10.3390/cancers12113097

11978  Asano N, Takeshima H, Yamashita S, Takamatsu H, Hattori N, Kubo T, Yoshida A, Kobayashi E, Nakayama R, Matsumoto M, Nakamura M, Ichikawa H, Kawai A, Kondo T, Ushijima T.  Epigenetic reprogramming underlies efficacy of DNA demethylation therapy in osteosarcomas.  Sci Rep  2019    PubMed ID: 31889115   DOI: 10.1038/s41598-019-56883-0

9465  Tomohiko Tokunaga, Takashi Ando, Miki Suzuki-Karasaki, Tomohisa Ito, Asuka Onoe-Takahashi, Toyoko Ochiai, Masayoshi Soma , Yoshihiro Suzuki-Karasaki  Plasma-stimulated Medium Kills TRAIL-resistant Human Malignant Cells by Promoting Caspase-Independent Cell Death via Membrane Potential and Calcium Dynamics Modulation  Int J Oncol  2018  52(3):697-708  PubMed ID: 29393427   DOI: 10.3892/ijo.2018.4251

14766  Wada KI, Hosokawa K, Ito Y, Maeda M.  Quantitative control of mitochondria transfer between live single cells using a microfluidic device  Biol Open  2017  6(12):1960-1965  PubMed ID: 29092814   DOI: 10.1242/bio.024869

10919    Antitumor activities of BIBF 1120, BI 860585, and BI 836845 in preclinical models of sarcoma  Integr Mol Med  2016  3: 755-760    DOI: 10.15761/IMM.1000239

16938  Odagiri H, Kadomatsu T, Endo M, Masuda T, Morioka MS, Fukuhara S, Miyamoto T, Kobayashi E, Miyata K, Aoi J, Horiguchi H, Nishimura N, Terada K, Yakushiji T, Manabe I, Mochizuki N, Mizuta H, Oike Y.  The secreted protein ANGPTL2 promotes metastasis of osteosarcoma cells through integrin α5β1, p38 MAPK, and matrix metalloproteinases  Sci Signal  2014  7(309):ra7  PubMed ID: 24448647   DOI: 10.1126/scisignal.2004612

18251  Rouleau C, Smale R, Fu YS, Hui G, Wang F, Hutto E, Fogle R, Jones CM, Krumbholz R, Roth S, Curiel M, Ren Y, Bagley RG, Wallar G, Miller G, Schmid S, Horten B, Teicher BA.  Endosialin is expressed in high grade and advanced sarcomas: evidence from clinical specimens and preclinical modeling  Int J Oncol  2011  39(1):73-89  PubMed ID: 21537839   DOI: 10.3892/ijo.2011.1020

8566  Morioka K, Tanikawa C, Ochi K, Daigo Y, Katagiri T, Kawano H, Kawaguchi H, Myoui A, Yoshikawa H, Naka N, Araki N, Kudawara I, Ieguchi M, Nakamura K, Nakamura Y, Matsuda K.  Orphan receptor tyrosine kinase ROR2 as a potential therapeutic target for osteosarcoma.  Cancer Sci.  2009  100:1227-33  PubMed ID: 19486338   DOI: 10.1111/j.1349-7006.2009.01165.x

9072  M Saijo, T Suzutani, K Mizuta, I Kurane, S Morikawa  Characterization and Susceptibility to Antiviral Agents of Herpes Simplex Virus Type 1 Containing a Unique Thymidine Kinase Gene With an Amber Codon Between the First and the Second Initiation Codons  Arch Virol  2008  153 (2), 303-14  PubMed ID: 18066636   DOI: 10.1007/s00705-007-1096-9

9041  Tatsuo Suzutani, Ken Ishioka, Erik De Clercq, Kei Ishibashi, Hisatoshi Kaneko, Toshihiko Kira, Koh-Ichi Hashimoto, Masahiro Ogasawara, Katsuki Ohtani, Nobutaka Wakamiya, Masayuki Saijo  Differential Mutation Patterns in Thymidine Kinase and DNA Polymerase Genes of Herpes Simplex Virus Type 1 Clones Passaged in the Presence of Acyclovir or Penciclovir  Antimicrob Agents Chemother  2003  47 (5), 1707-13  PubMed ID: 12709344   DOI: 10.1128/aac.47.5.1707-1713.2003

9034  Masayuki Saijo, Tatsuo Suzutani, Erik De Clercq, Masahiro Niikura, Akihiko Maeda, Shigeru Morikawa, Ichiro Kurane  Genotypic and Phenotypic Characterization of the Thymidine Kinase of ACV-resistant HSV-1 Derived From an Acyclovir-Sensitive Herpes Simplex Virus Type 1 Strain  Antiviral Res  2002  56 (3), 253-62  PubMed ID: 12406508   DOI: 10.1016/s0166-3542(02)00131-6

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