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Cell No. : Cell Name
RCB0700 : HTMM  update : 2024/01/17
CommentHuman cell line derived from malignant melanoma. Established from a metastasis to a lymph node. The origin is unknown.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Ishiwata, Isamu
Originator Ono, Isao & Isahiwata, Isamu &Isahikawa, Hiroshi
Year of deposit 1991
Animal _human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Female
Age at sampling 71 years
Tissue lymph node
Disease name melanoma
Classification cancer
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_8720
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Culture medium HamF12 + 15% FBS
Culture method
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) (-)
Isozyme LD, NP
Chromosome mode 40-150(50) : /47(5),48(1),49(7),50(4),51(14),52(7)/
STR(human) OK
Reference information Reference 1
User's Publication 1


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Reference
2293  Emi IIMURA, Tadao OHNO  EXAMINATION OF 254 PRIMATE CELL LINES BY A PCR PROTOCOL USEFUL FOR ROUTINE DETECTION OF LATENT HIV-I DNA  TISSUE CULTURE RESEARCH COMMUNICATIONS  1998  17:81-86   

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User's Publication
11645  Yamada H, Hakozaki M, Uemura A, Yamashita T.  Effect of fatty acids on melanogenesis and tumor cell growth in melanoma cells.  J. Lipid Res.  2019    PubMed ID: 31345992   DOI: 10.1194/jlr.M090712



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