Cell No. : Cell Name
RCB0662 : HOTHC
update : 2022/07/22
|
| Comment | Human cell line derived from undifferentiated carcinoma of thyroid (pleomorphous). Expressing tumor angiogenesis factor (TAF) and G-CSF. |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Ishiwata, Isamu
|
| Originator |
Ono, I. & Ishiwata, I. & Ishikawa, H.
|
| Year of deposit |
1991
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Gender |
Female
|
| Tissue |
thyroid
|
| Disease name |
anaplastic thyroid carcinoma
|
| Classification |
cancer
|
| Year of origin |
1982
|
|
Lifespan |
infinite
|
| Morphology |
epithelial-like
|
| Cellosaurus(Expasy) |
CVCL_8708
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
| Culture medium |
|
HamF12 + 15% FBS
|
| Antibiotics |
|
Free
|
|
Passage method |
|
0.25% Trypsin
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Culture information
|
Passage ratio |
|
1 : 8 split
|
| SC frequency |
|
Subculture : once/week, Medium Renewal : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma |
|
(-)
|
| Virus (HIV) |
|
(-)
|
| Isozyme |
|
LD, NP
|
| Chromosome mode |
|
66-164(50) : /79(6),80(8),81(3),82(8),83(1),84(3)/
|
| STR(human) |
|
OK
|
| Images |
deposit info | lot info |
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| Reference information |
Reference |
3
|
| User's Publication |
2
|
| Reference |
|
860
shiwata I, Ono I, Kiguchi K, Ishiwata C, Soma M, Ishikawa H.
Establishment and characterization of a human thyroid carcinoma cell line (HOTHC) producing colony stimulating factor
Hum Cell
2005
18(3):163-9
PubMed ID: 17022149
DOI: 10.1111/j.1749-0774.2005.tb00007.x
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47
Ishiwata I, Sudo T, Kiguchi K, Ishikawa H.
Tumor angiogenesis factors produced by cancer cells
Hum Cell
1999
;12(1):37-4
PubMed ID: 10457904
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1524
Ono I, Ishiwata I, Nakaguchi T, Konoe K, Tokita N, Soma M, Ishiwata C, Ishikawa H
[Heterotransplantability of human anaplastic thyroid carcinoma line (HOTHC) in nude mice, and biological properties of the heterograft]
Hum Cell
1989
2:74-9
PubMed ID: 2519207
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