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Cell No. : Cell Name
RCB0569 : 707.fl  update : 2024/12/24
CommentFriend leukemia cell line. High ability to differentiate to erythroblastoid cells.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Ikawa, Yoji
Originator Friend, C.
Year of deposit 1991
Animal _mouse < Mammals
Strain name DBA/2
Tissue spleen
Metastatic ability Yes
Classification cancer
History Friend, C. ->Ikawa, Y.
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_H711
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Culture method General Guidelines for Culturing Suspension Cells, 浮遊細胞の培養に関する一般的な注意(Japanese)
Culture medium RPMI1640 + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma/Acholeplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Reference information Reference 4
User's Publication 0


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Reference
3684  Todokoro K, Kanazawa S, Amanuma H, Ikawa Y.  Characterization of erythropoietin receptor on erythropoietin-unresponsive mouse erythroleukemia cells.  Biochim Biophys Acta  1988  943(2):326-30  PubMed ID: 2840960   DOI: 10.1016/0005-2736(88)90564-0
4775  Obinata M, Ikawa Y.  Change in message sequences during erythrodifferentiation.  Nucleic Acids Res  1980  8(18):4271-82  PubMed ID: 7433109   DOI: 10.1093/nar/8.18.4271
4773  Kameji R, Obinata M, Natori Y, Ikawa Y.  Induction of globin gene expression in cultured erythroleukemia cells by butyric acid.  J Biochem  1977  81(6):1901-10  PubMed ID: 268328   DOI: 10.1093/oxfordjournals.jbchem.a131652
4769  Harrison PR, Gilmour RS, Affara NA, Conkie D, Paul J.  Globin messenger RNA synthesis and processing during haemoglobin induction in Friend cells. II. Evidence for post-transcriptional control in clone 707  Cell Differ  1974  3(1):23-30  PubMed ID: 4527816   DOI: 10.1016/0045-6039(74)90037-2

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