Search
CELL BANK Website

Back Back
RCB0557 : B16 melanoma 4A5  update : 2018/07/31
CommentMelanin pigment producing mouse melanoma. Application consideration
Comment from the depositor
Terms and conditionsBasically, there is no restriction regarding academic use.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Sugimoto, Yoshinori
Year of deposit 1990
Original cell B16 melanoma
Animal mouse < Mammals
Strain name C57BL/6
Tissue skin
Case history melanoma
Classification cancer
Lifespan infinite
Morphology fibroblast-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 1-2 times/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP (mouse) OK
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 7
User's Publication 28


To topTop
Reference
547  Enzymatic determination of transmissible replicating factors associated with mouse tumors.  Riley,V.  Ann N Y Acad Sci  1963  100:762-790  PubMed ID: 13973915
587  Control of pigment production in mouse melanoma cells in vitro. Evocation and maintenance.  Silagi S  J Cell Biol  1969  43:263-74  PubMed ID: 4981070
3488  THE ISOLATION AND CYTOLOGY OF TWO PIGMENT CELL STRAINS FROM B-16 MOUSE MELANOMAS  HU F, LESNEY PF.  Cancer Res  1964  24:1634-43.  PubMed ID: 14234007
5063  Suppressive effect of spinach extract on the formation of melanin in B16 mouse melanoma cells.  Kobori M, Sasaki Y, Shinohara K.  Cytotechnology  1994  14(2):119-22  PubMed ID: 22358759
5064  Phloretin-induced apoptosis in B16 melanoma 4A5 cells by inhibition of glucose transmembrane transport.  Kobori M, Shinmoto H, Tsushida T, Shinohara K.  Cancer Lett  1997  119(2):207-12  PubMed ID: 9570373
5065  Phloretin-induced apoptosis in B16 melanoma 4A5 cells and HL60 human leukemia cells.  Kobori M, Iwashita K, Shinmoto H, Tsushida T.  Biosci Biotechnol Biochem  1999  63(4):719-25  PubMed ID: 10361685
5066  Flavonoids inhibit cell growth and induce apoptosis in B16 melanoma 4A5 cells.  Iwashita K, Kobori M, Yamaki K, Tsushida T.  Biosci Biotechnol Biochem  2000  64(9):1813-20  PubMed ID: 11055382

To topTop
User's Publication
2846  Therapeutic effect of dendritic cells loaded with a fusion mRNA encoding tyrosinase-related protein 2 and enhanced green fluorescence protein on B16 melanoma.  Fukui M, Nakano-Hashimoto T, Okano K, Maruta Y, Suehiro Y, Hamanaka Y, Yamashita H, Imai K, Kawano MM, Hinoda Y  Tumour Biol  2004  25:252-7  PubMed ID: 15627888
2368  Enhancement of tumoricidal activity of alveolar macrophages via CD40-CD40 ligand interaction  Imaizumi K, Kawabe T, Ichiyama S, Kikutani H, Yagita H, Shimokata K, Hasegawa Y  Am J Physiol  1999  277(1 Pt 1):L49-57  PubMed ID: 10409230
3221  Involvement of phospholipase D1 in melanogenesis of mouse B16 melanoma cells.  Ohguchi, Kenji, Banno, Yoshiko, Akao, Yukihiro, Nozawa, Yoshinori  J Biol Chem  2004  279:3408-12  PubMed ID: 14615486
3381  Induction of antigen-specific immune responses against malignant brain tumors by intramuscular injection of sindbis DNA encoding gp100 and IL-18.  Yamanaka, Ryuya, Xanthopoulos, Kleanthis G  DNA Cell Biol  2005  24:317-24  PubMed ID: 15869409
3313  Procyanidin trimers to pentamers fractionated from apple inhibit melanogenesis in B16 mouse melanoma cells.  Shoji, Toshihiko, Masumoto, Saeko, Moriichi, Nina, Kobori, Masuko, Kanda, Tomomasa, Shinmoto, Hiroshi, Tsushida, Tojiro  J Agric Food Chem  2005  53:6105-11  PubMed ID: 16029003
7101  Simultaneous quantitative analysis of 12 methoxyflavones with melanogenesis inhibitory activity from the rhizomes of Kaempferia parviflora.  Ninomiya K, Matsumoto T, Chaipech S, Miyake S, Katsuyama Y, Tsuboyama A, Pongpiriyadacha Y, Hayakawa T, Muraoka O, Morikawa T.  J Nat Med  2015  70:179-89  PubMed ID: 26711832
7182  Direct Melanoma Cell Contact Induces Stromal Cell Autocrine Prostaglandin E2-EP4 Receptor Signaling That Drives Tumor Growth, Angiogenesis, and Metastasis.  Inada M, Takita M, Yokoyama S, Watanabe K, Tominari T, Matsumoto C, Hirata M, Maru Y, Maruyama T, Sugimoto Y, Narumiya S, Uematsu S, Akira S, Murphy G, Nagase H, Miyaura C.  J. Biol. Chem.  2015  290:29781-93  PubMed ID: 26475855
7574  Phenotypic Characterization of a Microbe Producing Substances for Oxidative Stress Resistance Isolated from the Deep Seawater in Izu-Akazawa, Japan.  Yuji SHIBATA, Katsuhisa YAMADA, Chiaki IMADA, Takeshi KOBAYASHI, Takeshi TERAHARA  Deep Ocean Water Research  2015  15:117-124 
7618  Inhibitory effects of whisky polyphenols on melanogenesis in mouse B16 melanoma cells.  Yoshioka S, Terashita T, Yoshizumi H, Shirasaka N.  Biosci. Biotechnol. Biochem.  2011  75:2278-82  PubMed ID: 22146709
8108  Isolation of chemical constituents from Enicosanthum cupulare (King) Airy-Shaw.  Fujita S, Ninomiya M, Efdi M, Ohguchi K, Nozawa Y, Koketsu M.  Nat. Prod. Res.  2010  24:1630-6  PubMed ID: 20954090
8400  Inhibitory effects of flavonoid glycosides isolated from the peel of Japanese persimmon (Diospyros kaki 'Fuyu') on melanin biosynthesis.  Ohguchi K, Nakajima C, Oyama M, Iinuma M, Itoh T, Akao Y, Nozawa Y, Ito M.  Biol. Pharm. Bull.  2010  33:122-4  PubMed ID: 20045948
8513  Effect of Tunisian Capparis spinosa L. extract on melanogenesis in B16 murine melanoma cells.  Matsuyama K, Villareal MO, El Omri A, Han J, Kchouk ME, Isoda H.  J Nat Med  2009  63:468-72  PubMed ID: 19685105
8661  Effects of sesquiterpene lactones on melanogenesis in mouse B16 melanoma cells.  Ohguchi K, Ito M, Yokoyama K, Iinuma M, Itoh T, Nozawa Y, Akao Y.  Biol. Pharm. Bull.  2009  32:308-10  PubMed ID: 19182396
8715  Alpha-eleostearic acid and its dihydroxy derivative are major apoptosis-inducing components of bitter gourd.  Kobori M, Ohnishi-Kameyama M, Akimoto Y, Yukizaki C, Yoshida M.  J. Agric. Food Chem.  2008  56:10515-20  PubMed ID: 18959405
10603  3-O-Glyceryl-2-O-hexyl ascorbate suppresses melanogenesis by interfering with intracellular melanosome transport and suppressing tyrosinase protein synthesis.  Taira N, Katsuyama Y, Yoshioka M, Okano Y, Masaki H.  J Cosmet Dermatol  2018  17(6):1209-1215  PubMed ID: 29115012
10675  Labdane-Type Diterpenes, Galangalditerpenes A-C, with Melanogenesis Inhibitory Activity from the Fruit of Alpinia galanga.  Manse Y, Ninomiya K, Nishi R, Hashimoto Y, Chaipech S, Muraoka O, Morikawa T.  Molecules  2017  22:  PubMed ID: 29261124
10830  3-O-Glyceryl-2-O-hexyl Ascorbate Suppresses Melanogenesis through Activation of the Autophagy System.  Katsuyama Y, Taira N, Yoshioka M, Okano Y, Masaki H.  Biol. Pharm. Bull.  2018  41:824-827  PubMed ID: 29709921
11067  Compositions and melanogenesis-inhibitory activities of the extracts of defatted shea (Vitellaria paradoxa) kernels from seven African countries    Journal of Food Composition and Analysis  2018  70, 89-97 
11365  Photo-Modification of Melanin by a Mid-infrared Free-electron Laser.  Kawasaki T, Sato A, Tominaga Y, Suzuki Y, Oyama T, Tadokoro M, Tsukiyama K, Nokihara K, Zen H.  Photochem. Photobiol.  2019    PubMed ID: 30613988
11374  Anti-proliferative effect of auriculataoside A on B16 melanoma 4A5 cells by suppression of Cdc42-Rac1-RhoA signaling protein levels.  Wang W, Nakashima S, Nakamura S, Oda Y, Matsuda H.  J Nat Med  2019    PubMed ID: 30627935
11473  Inhibition of melanin production by anthracenone dimer glycosides isolated from Cassia auriculata seeds.  Wang W, Zhang Y, Nakashima S, Nakamura S, Wang T, Yoshikawa M, Matsuda H.  J Nat Med  2019    PubMed ID: 30847755
5330  Alkaloids from Sri Lankan curry-leaf (Murraya koenigii) display melanogenesis inhibitory activity: structures of karapinchamines A and B.  Nakamura S, Nakashima S, Oda Y, Yokota N, Fujimoto K, Matsumoto T, Ohta T, Ogawa K, Maeda S, Nishida S, Matsuda H, Yoshikawa M.  Bioorg Med Chem  2013  21(5):1043-9  PubMed ID: 23376010
5627  Melanogenesis inhibitory and fibroblast proliferation accelerating effects of noroleanane- and oleanane-type triterpene oligoglycosides from the flower buds of Camellia japonica.  Nakamura S, Moriura T, Park S, Fujimoto K, Matsumoto T, Ohta T, Matsuda H, Yoshikawa M.  J Nat Prod  2012  75(8):1425-30  PubMed ID: 22834923
5791  Selenoneine suppresses melanin synthesis by inhibiting tyrosinase in murine B16 melanoma cells and 3D-cultured human melanocytes  Takuya Seko, Shintaro Imamura, Kenji Ishihara, Yumiko Yamashita, Michiaki Yamashita  Fisheries Science   2019  1–9 
6255  Kappa-casein suppresses melanogenesis in cultured pigment cells.  Nakajima M, Shinoda I, Samejima Y, Miyauchi H, Fukuwatari Y, Hayasawa H.  Pigment Cell Res  1996  9(5):235-9  PubMed ID: 9014209
6287  Antiproliferative effect on human cancer cell lines after treatment with nimbolide extracted from an edible part of the neem tree (Azadirachta indica).  Roy MK, Kobori M, Takenaka M, Nakahara K, Shinmoto H, Isobe S, Tsushida T.  Phytother Res  2007  21(3):245-50  PubMed ID: 17163581
6320  Ectopic expression of syntaxin3 affects behaviors of B16 melanoma by controlling actin dynamics.  Shono M, Yoshioka R, Chatani Y, Hirai Y.  Cell Struct Funct  2013  38(1):97-107  PubMed ID: 23546178
3525  Mannosylerythritol lipid is a potent inducer of apoptosis and differentiation of mouse melanoma cells in culture.  Zhao X1, Wakamatsu Y, Shibahara M, Nomura N, Geltinger C, Nakahara T, Murata T, Yokoyama KK.  Cancer Res  1999  59(2):482-6  PubMed ID: 9927066



Back Back Return Top Page