Cell No. : Cell Name RCB0549 : Cle-H3  update : 2024/01/24
Comment			NIH3T3 transformant expressing human activated k-ras-2.
Comment from the depositor
Terms and conditions			There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	Takiguchi, Yuichi
		Originator	Miyamoto, Tadaaki
		Year of deposit	1990
		Another name	H-3, Cl-e
		Original cell	NIH3T3
		Animal	_mouse < Mammals
		Strain name	NIH Swiss
		Age at sampling	embryo
		Tissue	embryo
		Classification	transformed
		Recombinant	recombinant
		Exogene	MA cDNA
		Year of origin	1986
		Lifespan	infinite
		Morphology	fibroblast-like
		Cellosaurus(Expasy)	CVCL_F498
			deposit info	lot info
		Medium	Medium List
		Culture type		Adherent cells
		Culture medium		DMEM (low glucose) + 10% FBS
		Antibiotics		Free
		Passage method		0.25% Trypsin
	Culture information	Passage ratio		1 : 8 split
		SC frequency		Subculture : once/week, Medium Renewal : 2 times/week
		Temperature		37 ℃
		CO2 concentration		5 %
		Freeze medium		Medium + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma/Acholeplasma		(-)
		SSLP(mouse)		OK
		Isozyme		LD, NP
Images			deposit info	lot info
Reference information		Reference	3
		User's Publication	3

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Reference

2398  Takahashi Y, Takiguchi Y, Kuriyama T, Miyamoto T  Lattices of type I collagen select invasive variants of K-ras oncogene-transfected NIH3T3 with less cellular fibronectin.  Clin Exp Metastasis  1998  16:149-57  PubMed ID: 9514096   DOI: 10.1023/a:1021937102996

2397  Takiguchi Y, Kuriyama T, Miyamoto T  Evaluation of metastatic ability at specific times during primary tumor growth: a novel spontaneous metastasis assay.  Clin Exp Metastasis  1995  13:184-90  PubMed ID: 7750205   DOI: 10.1007/BF00132206

2396  Takiguchi Y, Takahashi Y, Kuriyama T, Miyamoto T  NIH3T3 transfectant containing human K-ras oncogene shows enhanced metastatic activity after in vivo tumor growth or co-culture with fibroblasts.  Clin Exp Metastasis  1992  10:351-60  PubMed ID: 1505125   DOI: 10.1007/BF00058175

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User's Publication

22324  Tsukiyama T, Zou J, Kim J, Ogamino S, Shino Y, Masuda T, Merenda A, Matsumoto M, Fujioka Y, Hirose T, Terai S, Takahashi H, Ishitani T, Nakayama KI, Ohba Y, Koo BK, Hatakeyama S.  A phospho-switch controls RNF43-mediated degradation of Wnt receptors to suppress tumorigenesis  Nat Commun  2020  11(1):4586.  PubMed ID: 32934222   DOI: 10.1038/s41467-020-18257-3

4123  Morisaku T, Ishihara M, Yui H.  Discrimination between Normal and Cancerous Cells from Dynamic Viscoelastic Properties with a Laser-induced Surface Deformation Microscope.  Anal Sci  2018    PubMed ID: 29780041   DOI: 10.2116/analsci.18P170

18003  Shrestha P, Sumitomo S, Lee CH, Nagahara K, Kamegai A, Yamanaka T, Takeuchi H, Kusakabe M, Mori M.  Tenascin: growth and adhesion modulation--extracellular matrix degrading function: an in vitro study  Eur J Cancer B Oral Oncol  1996  32B(2):106-13  PubMed ID: 8736172   DOI: 10.1016/0964-1955(95)00074-7