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RCB0548 : IA-5  update : 2018/07/06
CommentHuman large cell lung carcinoma. Taken from skin metastasis. In vivo-in vitro clonogenic assay.
Comment from the depositor
Use conditiona
Use condition descriptionBasically, there is no restriction regarding academic use.
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Takiguchi, Yuichi
Originator Miyamoto, Tadaaki, Takiguchi, Yuichi
Year of deposit 1990
Animal human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Male
Age at sampling 68 years
Tissue lung, skin meta
Metastastatic ability Yes
Metastatsd tissue skin, spleen
Classification cancer
Year of origin 1984
Memo_1 Mycoplasma was eliminated with MC-210.
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives HamF10 + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR (human) OK
Virus (HIV) (-)
Isozyme LD, NP
Chromosome mode 42-82(50) : /44(4),45(5),46(9),47(11),48(5),49(5)/
Images
deposit info
lot info
Reference information Reference 0
User's Publication 3


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Reference

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User's Publication
Yoshimasu, T. et al  Integrins α2β1,α5β1 and αvβ5 are related to tumor growth and metastasis of non-small cell cancer   Japanese Journal of Lung Cancer  2001  41:111-115 
Yoshimasu, Tatsuya, Sakurai, Teruhisa, Oura, Shoji, Hirai, Issei, Tanino, Hirokazu, Kokawa, Yozo, Naito, Yasuaki, Okamura, Yoshitaka, Ota, Ichiro, Tani, Naoyuki, Matsuura, Nariaki  Increased expression of integrin alpha3beta1 in highly brain metastatic subclone of a human non-small cell lung cancer cell line.  Cancer Sci  2004  95:142-8 
Yoshimasu T, Sakurai T, Oura S, Hirai I, Tanino H, Kokawa Y, Naito Y, Okamura Y, Ota I, Tani N, Matsuura N.  Increased expression of integrin alpha3beta1 in highly brain metastatic subclone of a human non-small cell lung cancer cell line.  Cancer Sci.  2004  95:142-8  PubMed ID: 14965364



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