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RCB0526 : Jyg-MC(A)  update : 2018/07/06
Commentmurine mammary tumor cell lines. High level MMTV producer line.
Comment from the depositor
Terms and conditionsBasically, there is no restriction regarding academic use.
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.
Basic information Depositor Morimoto, Junji
Originator Morimoto, Junji
Year of deposit 1990
Animal mouse < Mammals
Strain name JYG
Gender Female
Tissue mammary
Case history mammary tumor
Metastastatic ability Yes
Metastatsd tissue lung, liver,spleen
Classification cancer
Year of origin 1987
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP, G6PD
Chromosome mode 38-126(50) : /39(7),40(11),41(4),55(1),58(2),59(1)/
deposit info
lot info
Reference information Reference 1
User's Publication 4

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1432  Morimoto J, Imai S, Haga S, Iwai Y, Iwai M, Hiroishi S, Miyashita N, Moriwaki K, Hosick, H L  New murine mammary tumor cell lines.  In Vitro Cell Dev Biol  1991  27A:349-51  PubMed ID: 2071538

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User's Publication
10266  San TH, Fujisawa M, Fushimi S, Yoshimura T, Ohara T, Soe L, Min NW, Kyaw O, Yang X, Matsukawa A.  Low prevalence of human mammary tumor virus (HMTV) in breast cancer patients from Myanmar.  Infect. Agents Cancer  2017  12:20  PubMed ID: 28413435
7147  Hiraga T, Ito S, Nakamura H.  EpCAM expression in breast cancer cells is associated with enhanced bone metastasis formation.  Int. J. Cancer  2015  138:1698-708  PubMed ID: 26576938
8619  Lee TL, Li Y, Alba D, Vong QP, Wu SM, Baxendale V, Rennert OM, Lau YF, Chan WY.  Developmental staging of male murine embryonic gonad by SAGE analysis.  J Genet Genomics  2009  36:215-27  PubMed ID: 19376482
3952  Akatsu T, Ono K, Katayama Y, Tamura T, Nishikawa M, Kugai N, Yamamoto M, Nagata N.  The mouse mammary tumor cell line, MMT060562, produces prostaglandin E2 and leukemia inhibitory factor and supports osteoclast formation in vitro via a stromal cell-dependent pathway.  J Bone Miner Res  1998  13(3):400-8  PubMed ID: 9525340

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