細胞番号 : 細胞名
RCB0513 : SKN
update : 2023/09/04
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| 細胞特性(Comment:英) | Human cell line derived from uterine leiomyosarcoma. Sensitive to estradiol-17 beta. Metastasis to kidney was observed in the clinic. |
| 細胞特性(日) | ヒト子宮平滑筋肉腫由来細胞株。Estradiol-17 betaに感受性がある。臨床的には腎転移が観られた。 |
| 細胞特性(寄託者記述:英) | |
| 細胞特性(寄託者記述:日) | |
| 使用条件(英) | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| 使用条件(日) | 基礎的研究につきましては、使用制限はありません。商業利用や特許取得を目的とする場合は、事前に必ず理研細胞バンクに連絡をすること。 |
| 備考(英) | |
| 備考(日) | |
| 提供申込書類(英) |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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| 提供申込書類(日) |
依頼書C-0001.pdf
同意書(非営利学術目的)C-0003.pdf
同意書(営利目的)C-0003p.pdf
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提供同意書は、使用機関の種類や目的に応じて、非営利学術目的 (C-XXXX) と営利目的 (C-XXXXp) の2種類があります。該当する提供同意書をご使用ください（詳細）。特許等の取得及び商業利用等は事前に必ず cellbank.brc@riken.jp までご連絡ください。
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| 提供手数料 |
手数料とお支払いについてはこちらをご覧ください。 |
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細胞基本情報
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|
寄託者 |
Ishiwata, Isamu
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| 樹立者 |
Ishiwata, Isamu
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| 寄託日 |
1990
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| 動物種 |
_human
< Mammals
|
| 属名 |
Homo
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| 種名 |
sapiens
|
| 性別 |
Female
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| 齢 |
52 years
|
| 採取組織 |
endometrium
|
| 転移 |
Yes
|
| 転移巣 |
kidney
|
| 細胞分類 |
cancer
|
| 初代培養日 |
1975
|
| メモ_1 |
Mycoplasma was eliminated with MC-210.
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|
細胞寿命 |
infinite
|
| 細胞形態 |
fibroblast-like
|
| Cellosaurus(Expasy) |
CVCL_3167
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|
細胞培養・検査情報
| |
寄託時情報 |
ロット情報 |
| 培地・試薬情報 |
培地・試薬一覧はこちらをご覧ください。 |
|
培養形態 |
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Adherent cells
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| 培地 |
|
HamF12 + 15% FBS
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| 抗生物質 |
|
Free
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|
継代方法 |
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0.25% Trypsin
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|
継代密度 |
|
1 : 8 split
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| 継代・培地交換頻度 |
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Subculture : 2 times/week
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| 培養最適温度 |
|
37
℃
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| 二酸化炭素濃度 |
|
5
%
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| 凍結培地 |
|
Medium + 10% DMSO
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| 凍結方法 |
|
Slow freezing
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| マイコプラズマ |
|
(-)
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| ウィルス（HIV) |
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(-)
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| アイソザイム検査 |
|
LD, NP, G6PD, AST, MPI
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| 染色体検査 |
|
80-207(50) : 80(7),81(8),82(6),83(12),84(5),85(2)/
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| 個体識別検査 |
|
OK
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寄託時情報
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|
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ロット情報
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| D5S818 |
D13S317 |
D7S820 |
D16S539 |
vWA |
TH01 |
Amelogenin |
TPOX |
CSF1PO |
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12,13
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11
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8,12
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9
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19
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8
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X
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8
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10,11
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| 画像情報 |
寄託時情報 | ロット情報 |
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| 文献情報 |
Reference(英) |
6件
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| Reference(日) |
0件
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| 利用者成果(英) |
8件
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| 利用者成果(日) |
0件
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| Reference(英) |
|
257
Withanage GS, Murata H, Koyama T, Ishiwata I.
Agonistic and antagonistic effects of zearalenone, an etrogenic mycotoxin, on SKN, HHUA, and HepG2 human cancer cell lines
Vet Hum Toxicol
2001
43(1):6-10
PubMed ID: 11205083
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261
Ishiwata I, Ishiwata C, Nozawa S, Iizuka R, Ishikawa H.
Change in leukocyte count in peripheral blood of nude mouse bearing transplanted gynecological malignant tumors
Nihon Sanka Fujinka Gakkai Zasshi
1988
40(11):1767-71
PubMed ID: 2466923
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1035
Ishiwata I, Ishiwata C, Soma M, Ono I, Nakaguchi T, Ishikawa H.
Tumor angiogenic activity of gynecologic tumor cell lines on the chorioallantoic membrane
Gynecol Oncol
1988
29(1):87-93
PubMed ID: 2448191
DOI: 10.1016/0090-8258(88)90151-5
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1198
Ishiwata I, Ishiwata C, Ishikawa H
Biological characteristics of a human uterine leiomyosarcoma cell line,particularly its heterotransplantability and effect on the proliferation of vascular endothelial cells.
Nippon Sanka Fujinka Gakkai Zasshi
1987
39:853-8
PubMed ID: 2439619
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259
Ishiwata I, Ishiwata C, Ishikawa H.
Effects of sex-steroid hormones on growth and hormone receptor levels of human uterine leiomyosarcoma cells in vitro
Asia Oceania J Obstet Gynaecol
1982
9(2):207-15
PubMed ID: 6615335
DOI: 10.1111/j.1447-0756.1983.tb00624.x
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1201
Ishiwata I, Nozawa S, Nagai S, Kurihara S, Mikata A
Establishment of a human leiomyosarcoma cell line.
Cancer Res
1977
37:658-64
PubMed ID: 556976
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| 利用者成果(英) |
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7548
Miyata T, Sonoda K, Tomikawa J, Tayama C, Okamura K, Maehara K, Kobayashi H, Wake N, Kato K, Hata K, Nakabayashi K.
Genomic, Epigenomic, and Transcriptomic Profiling towards Identifying Omics Features and Specific Biomarkers That Distinguish Uterine Leiomyosarcoma and Leiomyoma at Molecular Levels.
Sarcoma
2015
2015:412068
PubMed ID: 27057136
DOI: 10.1155/2015/412068
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18111
Tanaka T, Toujima S, Toyoda S, Takeuchi S, Umesaki N.
Establishment and characterization of novel human uterine leiomyosarcoma cell lines
Int J Oncol
2010
37(1):125-31.
PubMed ID: 20514404
DOI: 10.3892/ijo_00000660
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14017
Tanaka T, Bai T, Yukawa K.
Death-associated protein kinase is essential for the survival of various types of uterine cancer cells
Int J Oncol
2010
37(4):1017-22
PubMed ID: 20811724
DOI: 10.3892/ijo_00000753
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10358
Iwao Ishiwata, Makoto Yasuda, Takashi Hirano & Hiroshi Ishikawa
Establishment and characterization of HEPFT, a cell line derived from hepatoid carcinoma of the fallopian tube, with special reference to α-fetoprotein, lectin affinity and histogenesis
Human Cell
2007
20:119–130
PubMed ID: 17949352
DOI: 10.1111/j.1749-0774.2007.00039.x
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8893
Hayashi T, Kobayashi Y, Kohsaka S, Sano K.
The mutation in the ATP-binding region of JAK1, identified in human uterine leiomyosarcomas, results in defective interferon-gamma inducibility of TAP1 and LMP2.
Oncogene
2006
25:4016-26
PubMed ID: 16474838
DOI: 10.1038/sj.onc.1209434
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9030
H Yamamura, M Hashio, M Noguchi, Y Sugenoya, M Osakada, N Hirano, Y Sasaki, T Yoden, N Awata, N Araki, M Tatsuta, S I Miyatake, K Takahashi
Identification of the Transcriptional Regulatory Sequences of Human Calponin Promoter and Their Use in Targeting a Conditionally Replicating Herpes Vector to Malignant Human Soft Tissue and Bone Tumors
Cancer Res
2001
61 (10), 3969-77
PubMed ID: 11358814
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2061
Sugimoto H, Nishino H
[Effect of recombinant human basic fibroblast growth factor (bFGF) on the growth of human tumor cell lines]
Hum Cell
1996
9:129-40
PubMed ID: 9183641
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17977
Kagami I, Mizunuma H, Miyamoto S, Ibuki Y, Uchida T.
Quantification of estrogen receptor messenger RNA by quantitative polymerase chain reaction using internal standard fragment
Biochem Biophys Res Commun
1996
228(2):358-64
PubMed ID: 8920919
DOI: 10.1006/bbrc.1996.1666
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