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RCB0513 : SKN  update : 2018/07/06
CommentEstradiol-17 beta responsive leiomyosarcoma
Comment from the depositor
Terms and conditionsBasically, there is no restriction regarding academic use.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Ishiwata, Isamu
Originator Ishiwata, Isamu
Year of deposit 1990
Animal human < Mammals
Genus Homo
Species sapiens
Gender Female
Age at sampling 52 years
Tissue endometrium
Metastastatic ability Yes
Metastatsd tissue kidney
Classification cancer
Year of origin 1975
Memo_1 Mycoplasma was eliminated with MC-210.
Lifespan infinite
Morphology fibroblast-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives HamF12 + 15% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR (human) OK
Virus (HIV) (-)
Isozyme LD, NP, G6PD, AST, MPI
Chromosome mode 80-207(50) : 80(7),81(8),82(6),83(12),84(5),85(2)/
Images
deposit info
lot info
Reference information Reference 2
User's Publication 3


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Reference
1201  Ishiwata I, Nozawa S, Nagai S, Kurihara S, Mikata A  Establishment of a human leiomyosarcoma cell line.  Cancer Res  1977  37:658-64  PubMed ID: 556976
1198  Ishiwata I, Ishiwata C, Ishikawa H  Biological characteristics of a human uterine leiomyosarcoma cell line,particularly its heterotransplantability and effect on the proliferation of vascular endothelial cells.  Nippon Sanka Fujinka Gakkai Zasshi  1987  39:853-8  PubMed ID: 2439619

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User's Publication
2061  Sugimoto H, Nishino H  [Effect of recombinant human basic fibroblast growth factor (bFGF) on the growth of human tumor cell lines]  Hum Cell  1996  9:129-40  PubMed ID: 9183641
7548  Miyata T, Sonoda K, Tomikawa J, Tayama C, Okamura K, Maehara K, Kobayashi H, Wake N, Kato K, Hata K, Nakabayashi K.  Genomic, Epigenomic, and Transcriptomic Profiling towards Identifying Omics Features and Specific Biomarkers That Distinguish Uterine Leiomyosarcoma and Leiomyoma at Molecular Levels.  Sarcoma  2015  2015:412068  PubMed ID: 27057136
8893  Hayashi T, Kobayashi Y, Kohsaka S, Sano K.  The mutation in the ATP-binding region of JAK1, identified in human uterine leiomyosarcomas, results in defective interferon-gamma inducibility of TAP1 and LMP2.  Oncogene  2006  25:4016-26  PubMed ID: 16474838



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