Cell No. : Cell Name
RCB0513 : SKN
update : 2024/08/13
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| Comment | Human cell line derived from uterine leiomyosarcoma. Sensitive to estradiol-17 beta. Metastasis to kidney was observed in the clinic. |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Ishiwata, Isamu
|
| Originator |
Ishiwata, Isamu
|
| Year of deposit |
1990
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Gender |
Female
|
| Age at sampling |
52 years
|
| Tissue |
endometrium
|
| Metastatic ability |
Yes
|
| Metastatic tissue |
kidney
|
| Classification |
cancer
|
| Year of origin |
1975
|
| Memo_1 |
Mycoplasma was eliminated with MC-210.
|
|
Lifespan |
infinite
|
| Morphology |
fibroblast-like
|
| Cellosaurus(Expasy) |
CVCL_3167
|
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| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
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| Culture medium |
|
HamF12 + 15% FBS
|
| Antibiotics |
|
Free
|
|
Passage method |
|
0.25% Trypsin
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Culture information
|
Passage ratio |
|
1 : 8 split
|
| SC frequency |
|
Subculture : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| Virus (HIV) |
|
(-)
|
| Isozyme |
|
LD, NP, G6PD, AST, MPI
|
| Chromosome mode |
|
80-207(50) : 80(7),81(8),82(6),83(12),84(5),85(2)/
|
| STR(human) |
|
OK
|
|
deposit info
|
|
|
lot info
|
| D5S818 |
D13S317 |
D7S820 |
D16S539 |
vWA |
TH01 |
Amelogenin |
TPOX |
CSF1PO |
|
12,13
|
11
|
8,12
|
9
|
19
|
8
|
X
|
8
|
10,11
|
|
| Images |
deposit info | lot info |
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| Reference information |
Reference |
6
|
| User's Publication |
8
|
| Reference |
|
257
Withanage GS, Murata H, Koyama T, Ishiwata I.
Agonistic and antagonistic effects of zearalenone, an etrogenic mycotoxin, on SKN, HHUA, and HepG2 human cancer cell lines
Vet Hum Toxicol
2001
43(1):6-10
PubMed ID: 11205083
|
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261
Ishiwata I, Ishiwata C, Nozawa S, Iizuka R, Ishikawa H.
Change in leukocyte count in peripheral blood of nude mouse bearing transplanted gynecological malignant tumors
Nihon Sanka Fujinka Gakkai Zasshi
1988
40(11):1767-71
PubMed ID: 2466923
|
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1035
Ishiwata I, Ishiwata C, Soma M, Ono I, Nakaguchi T, Ishikawa H.
Tumor angiogenic activity of gynecologic tumor cell lines on the chorioallantoic membrane
Gynecol Oncol
1988
29(1):87-93
PubMed ID: 2448191
DOI: 10.1016/0090-8258(88)90151-5
|
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1198
Ishiwata I, Ishiwata C, Ishikawa H
Biological characteristics of a human uterine leiomyosarcoma cell line,particularly its heterotransplantability and effect on the proliferation of vascular endothelial cells.
Nippon Sanka Fujinka Gakkai Zasshi
1987
39:853-8
PubMed ID: 2439619
|
|
259
Ishiwata I, Ishiwata C, Ishikawa H.
Effects of sex-steroid hormones on growth and hormone receptor levels of human uterine leiomyosarcoma cells in vitro
Asia Oceania J Obstet Gynaecol
1982
9(2):207-15
PubMed ID: 6615335
DOI: 10.1111/j.1447-0756.1983.tb00624.x
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1201
Ishiwata I, Nozawa S, Nagai S, Kurihara S, Mikata A
Establishment of a human leiomyosarcoma cell line.
Cancer Res
1977
37:658-64
PubMed ID: 556976
|
| User's Publication |
|
7548
Miyata T, Sonoda K, Tomikawa J, Tayama C, Okamura K, Maehara K, Kobayashi H, Wake N, Kato K, Hata K, Nakabayashi K.
Genomic, Epigenomic, and Transcriptomic Profiling towards Identifying Omics Features and Specific Biomarkers That Distinguish Uterine Leiomyosarcoma and Leiomyoma at Molecular Levels.
Sarcoma
2015
2015:412068
PubMed ID: 27057136
DOI: 10.1155/2015/412068
|
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18111
Tanaka T, Toujima S, Toyoda S, Takeuchi S, Umesaki N.
Establishment and characterization of novel human uterine leiomyosarcoma cell lines
Int J Oncol
2010
37(1):125-31.
PubMed ID: 20514404
DOI: 10.3892/ijo_00000660
|
|
14017
Tanaka T, Bai T, Yukawa K.
Death-associated protein kinase is essential for the survival of various types of uterine cancer cells
Int J Oncol
2010
37(4):1017-22
PubMed ID: 20811724
DOI: 10.3892/ijo_00000753
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10358
Iwao Ishiwata, Makoto Yasuda, Takashi Hirano & Hiroshi Ishikawa
Establishment and characterization of HEPFT, a cell line derived from hepatoid carcinoma of the fallopian tube, with special reference to α-fetoprotein, lectin affinity and histogenesis
Human Cell
2007
20:119–130
PubMed ID: 17949352
DOI: 10.1111/j.1749-0774.2007.00039.x
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8893
Hayashi T, Kobayashi Y, Kohsaka S, Sano K.
The mutation in the ATP-binding region of JAK1, identified in human uterine leiomyosarcomas, results in defective interferon-gamma inducibility of TAP1 and LMP2.
Oncogene
2006
25:4016-26
PubMed ID: 16474838
DOI: 10.1038/sj.onc.1209434
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9030
H Yamamura, M Hashio, M Noguchi, Y Sugenoya, M Osakada, N Hirano, Y Sasaki, T Yoden, N Awata, N Araki, M Tatsuta, S I Miyatake, K Takahashi
Identification of the Transcriptional Regulatory Sequences of Human Calponin Promoter and Their Use in Targeting a Conditionally Replicating Herpes Vector to Malignant Human Soft Tissue and Bone Tumors
Cancer Res
2001
61 (10), 3969-77
PubMed ID: 11358814
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2061
Sugimoto H, Nishino H
[Effect of recombinant human basic fibroblast growth factor (bFGF) on the growth of human tumor cell lines]
Hum Cell
1996
9:129-40
PubMed ID: 9183641
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17977
Kagami I, Mizunuma H, Miyamoto S, Ibuki Y, Uchida T.
Quantification of estrogen receptor messenger RNA by quantitative polymerase chain reaction using internal standard fragment
Biochem Biophys Res Commun
1996
228(2):358-64
PubMed ID: 8920919
DOI: 10.1006/bbrc.1996.1666
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