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Cell No. : Cell Name
RCB0513 : SKN  update : 2025/04/25
CommentHuman cell line derived from uterine leiomyosarcoma. Sensitive to estradiol-17 beta. Metastasis to kidney was observed in the clinic.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Ishiwata, Isamu
Originator Ishiwata, Isamu
Year of deposit 1990
Animal _human < Mammals
Genus Homo
Species sapiens
Gender Female
Age at sampling 52 years
Tissue endometrium
Metastatic ability Yes
Metastatic tissue kidney
Classification cancer
Year of origin 1975
Memo_1 Mycoplasma was eliminated with MC-210.
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_3167
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium HamF12 + 15% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma/Acholeplasma (-)
Virus (HIV) (-)
Isozyme LD, NP, G6PD, AST, MPI
Chromosome mode 80-207(50) : 80(7),81(8),82(6),83(12),84(5),85(2)/
STR(human) OK
deposit info
lot info
D5S818 D13S317 D7S820 D16S539 vWA TH01 Amelogenin TPOX CSF1PO
12,13 11 8,12 9 19 8 X 8 10,11
Images
deposit info
lot info
Reference information Reference 6
User's Publication 9


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Reference
257  Withanage GS, Murata H, Koyama T, Ishiwata I.  Agonistic and antagonistic effects of zearalenone, an etrogenic mycotoxin, on SKN, HHUA, and HepG2 human cancer cell lines  Vet Hum Toxicol  2001  43(1):6-10  PubMed ID: 11205083  
261  Ishiwata I, Ishiwata C, Nozawa S, Iizuka R, Ishikawa H.  Change in leukocyte count in peripheral blood of nude mouse bearing transplanted gynecological malignant tumors  Nihon Sanka Fujinka Gakkai Zasshi  1988  40(11):1767-71  PubMed ID: 2466923  
1035  Ishiwata I, Ishiwata C, Soma M, Ono I, Nakaguchi T, Ishikawa H.  Tumor angiogenic activity of gynecologic tumor cell lines on the chorioallantoic membrane  Gynecol Oncol  1988  29(1):87-93  PubMed ID: 2448191   DOI: 10.1016/0090-8258(88)90151-5
1198  Ishiwata I, Ishiwata C, Ishikawa H  Biological characteristics of a human uterine leiomyosarcoma cell line,particularly its heterotransplantability and effect on the proliferation of vascular endothelial cells.  Nippon Sanka Fujinka Gakkai Zasshi  1987  39:853-8  PubMed ID: 2439619  
259  Ishiwata I, Ishiwata C, Ishikawa H.  Effects of sex-steroid hormones on growth and hormone receptor levels of human uterine leiomyosarcoma cells in vitro  Asia Oceania J Obstet Gynaecol  1982  9(2):207-15  PubMed ID: 6615335   DOI: 10.1111/j.1447-0756.1983.tb00624.x
1201  Ishiwata I, Nozawa S, Nagai S, Kurihara S, Mikata A  Establishment of a human leiomyosarcoma cell line.  Cancer Res  1977  37:658-64  PubMed ID: 556976  

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User's Publication
7548  Miyata T, Sonoda K, Tomikawa J, Tayama C, Okamura K, Maehara K, Kobayashi H, Wake N, Kato K, Hata K, Nakabayashi K.  Genomic, Epigenomic, and Transcriptomic Profiling towards Identifying Omics Features and Specific Biomarkers That Distinguish Uterine Leiomyosarcoma and Leiomyoma at Molecular Levels.  Sarcoma  2015  2015:412068  PubMed ID: 27057136   DOI: 10.1155/2015/412068
18111  Tanaka T, Toujima S, Toyoda S, Takeuchi S, Umesaki N.  Establishment and characterization of novel human uterine leiomyosarcoma cell lines  Int J Oncol  2010  37(1):125-31.  PubMed ID: 20514404   DOI: 10.3892/ijo_00000660
14017  Tanaka T, Bai T, Yukawa K.  Death-associated protein kinase is essential for the survival of various types of uterine cancer cells  Int J Oncol  2010  37(4):1017-22  PubMed ID: 20811724   DOI: 10.3892/ijo_00000753
10358  Iwao Ishiwata, Makoto Yasuda, Takashi Hirano & Hiroshi Ishikawa   Establishment and characterization of HEPFT, a cell line derived from hepatoid carcinoma of the fallopian tube, with special reference to α-fetoprotein, lectin affinity and histogenesis  Human Cell  2007  20:119–130  PubMed ID: 17949352   DOI: 10.1111/j.1749-0774.2007.00039.x
8893  Hayashi T, Kobayashi Y, Kohsaka S, Sano K.  The mutation in the ATP-binding region of JAK1, identified in human uterine leiomyosarcomas, results in defective interferon-gamma inducibility of TAP1 and LMP2.  Oncogene  2006  25:4016-26  PubMed ID: 16474838   DOI: 10.1038/sj.onc.1209434
9030  H Yamamura, M Hashio, M Noguchi, Y Sugenoya, M Osakada, N Hirano, Y Sasaki, T Yoden, N Awata, N Araki, M Tatsuta, S I Miyatake, K Takahashi  Identification of the Transcriptional Regulatory Sequences of Human Calponin Promoter and Their Use in Targeting a Conditionally Replicating Herpes Vector to Malignant Human Soft Tissue and Bone Tumors  Cancer Res  2001  61 (10), 3969-77  PubMed ID: 11358814  
2007  Greenland KJ, Jantke I, Jenatschke S, Bracken KE, Vinson C, Gellersen B.  The human NAD+-dependent 15-hydroxyprostaglandin dehydrogenase gene promoter is controlled by Ets and activating protein-1 transcription factors and progesterone  Endocrinology  2000  141(2):581-97  PubMed ID: 10650939   DOI: 10.1210/endo.141.2.7313
2061  Sugimoto H, Nishino H  [Effect of recombinant human basic fibroblast growth factor (bFGF) on the growth of human tumor cell lines]  Hum Cell  1996  9:129-40  PubMed ID: 9183641  
17977  Kagami I, Mizunuma H, Miyamoto S, Ibuki Y, Uchida T.  Quantification of estrogen receptor messenger RNA by quantitative polymerase chain reaction using internal standard fragment  Biochem Biophys Res Commun  1996  228(2):358-64  PubMed ID: 8920919   DOI: 10.1006/bbrc.1996.1666


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