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RCB0495 : KU812  update : 2018/07/06
CommentChronic myelogeneous leukemia, Ph1 chromosome (+), basophil-like cells.
Comment from the depositor
Restriction (Category)a
Terms and conditionsBasically, there is no restriction regarding academic use.
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.
Basic information Depositor Mizusawa, Hiroshi
Originator Kishi, Kenji
Year of deposit 1990
Animal human < Mammals
Genus Homo
Species sapiens
Gender Male
Tissue blood
Classification cancer
History JCRB0104
Lifespan infinite
Morphology lymphocyte-like
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR (human) OK
Virus (HIV) (-)
Virus (HTLV-1) (-)
Isozyme LD, NP
Chromosome mode 50-108(50) : /58(1),59(4),60(8),61(5),62(5),63(9)/
deposit info
lot info
Reference information Reference 2
User's Publication 8

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1796  Valent P, Besemer J, Sillaber C, Butterfield, J H, Eher R, Majdic O, Kishi K, Klepetko W, Eckersberger F, Lechner K, et al.  Failure to detect IL-3-binding sites on human mast cells.  J Immunol  1990  145:3432-7  PubMed ID: 2230127
1795  Nakazawa M, Mitjavila, M T, Debili N, Casadevall N, Mayeux P, Rouyer-Fessard P, Dubart A, Romeo, P H, Beuzard Y, Kishi K, et al.  KU 812: a pluripotent human cell line with spontaneous erythroid terminal maturation.  Blood  1989  73:2003-13  PubMed ID: 2540861

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User's Publication
2665  Iwasaki A, Doi T, Umetani M, Watanabe M, Suda M, Hattori Y, Nagoya T  Affinity improvement of the high-affinity immunoglobulin E receptor by phage display.  Biochem Biophys Res Commun  2002  293:542-8  PubMed ID: 12054635
2848  Feng, Xingmin, Chuhjo, Tatsuya, Sugimori, Chiharu, Kotani, Takeharu, Lu, Xuzhang, Takami, Akiyoshi, Takamatsu, Hiroyuki, Yamazaki, Hirohito, Nakao, Shinji  Diazepam-binding inhibitor-related protein 1: a candidate autoantigen in acquired aplastic anemia patients harboring a minor population of paroxysmal nocturnal hemoglobinuria-type cells.  Blood  2004  104:2425-31  PubMed ID: 15217832
3164  Takamatsu, Shinji, Inoue, Noboru, Katsumata, Toshiyuki, Nakamura, Katsumi, Fujibayashi, Yasuhisa, Takeuchi, Makoto  The relationship between the branch-forming glycosyltransferases and cell surface sugar chain structures.  Biochemistry  2005  44:6343-9  PubMed ID: 15835923
7368  Liao EC, Chang CY, Hsieh CW, Yu SJ, Yin SC, Tsai JJ.  An Exploratory Pilot Study of Genetic Marker for IgE-Mediated Allergic Diseases with Expressions of FcεR1α and Cε.  Int J Mol Sci  2015  16:9504-19  PubMed ID: 25923080
7936  Motojima H, O Villareal M, Han J, Isoda H.  Microarray analysis of immediate-type allergy in KU812 cells in response to fulvic acid.  Cytotechnology  2011  63:181-90  PubMed ID: 21331654
8071  Yamada P, Nemoto M, Shigemori H, Yokota S, Isoda H.  Isolation of 5-(hydroxymethyl)furfural from Lycium chinense and its inhibitory effect on the chemical mediator release by basophilic cells.  Planta Med.  2010  77:434-40  PubMed ID: 21031336
8314  Yamada P, Iijima R, Han J, Shigemori H, Yokota S, Isoda H.  Inhibitory effect of acteoside isolated from Cistanche tubulosa on chemical mediator release and inflammatory cytokine production by RBL-2H3 and KU812 cells.  Planta Med.  2010  76:1512-8  PubMed ID: 20354949
8529  Wu H, Qi H, Iwasaki D, Zhu B, Shimoishi Y, Murata Y, Nakamura Y.  JNK-dependent NFATc1 pathway positively regulates IL-13 gene expression induced by (-)-epigallocatechin-3-gallate in human basophilic KU812 cells.  Free Radic. Biol. Med.  2009  47:1028-38  PubMed ID: 19607912

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