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RCB0484 : LA-N-2  update : 2019/01/18
CommentNeuroblastoma. N-myc gene amplification.
Comment from the depositor
Restriction (Category)f
Terms and conditionsNon-profit institutions only.
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.
Basic information Depositor Nishi, Yoshisuke
Originator Seeger, Robert C.
Year of deposit 1990
Animal human < Mammals
Genus Homo
Species sapiens
Gender Female
Age at sampling 3 years
Tissue nervous system
Case history neuroblastoma (stage IV)
Metastastatic ability Yes
Metastatsd tissue abdominal mass
Classification cancer
History Latt, S. A.
Lifespan infinite
Morphology other
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method pipetting or 0.25% trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : once/10 days, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR (human) OK
Virus (HIV) (-)
Isozyme LD, NP, G6PD, AST, MPI
Chromosome mode 70-152(50) : /74(2),75(11),76(13),77(4),78(6),79(4)/
deposit info
lot info
Reference information Reference 1
User's Publication 4

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1122  Seeger, R C, Rayner, S A, Banerjee A, Chung H, Laug, W E, Neustein, H B, Benedict, W F  Morphology, growth, chromosomal pattern and fibrinolytic activity of two new human neuroblastoma cell lines.  Cancer Res  1977  37:1364-71  PubMed ID: 856461

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User's Publication
1969  Maruyama K, Tsukada T, Bandoh S, Sasaki K, Ohkura N, Yamaguchi K  Expression of NOR-1 and its closely related members of the steroid/thyroid hormone receptor superfamily in human neuroblastoma cell lines.  Cancer Lett  1995  96:117-22  PubMed ID: 7553599
2214  Katayama M, Iwamatsu A, Masutani H, Furuke K, Takeda K, Wada H, Masuda T, Ishii K  Expression of neural cell adhesion molecule L1 in human lung cancer cell lines.  Cell Struct Funct  1997  22:511-6  PubMed ID: 9431455
8022  Yamada T, Inazu M, Tajima H, Matsumiya T.  Functional expression of choline transporter-like protein 1 (CTL1) in human neuroblastoma cells and its link to acetylcholine synthesis.  Neurochem. Int.  2010  58:354-65  PubMed ID: 21185344
8802  Inoue K, Ito K, Osato M, Lee B, Bae SC, Ito Y.  The transcription factor Runx3 represses the neurotrophin receptor TrkB during lineage commitment of dorsal root ganglion neurons.  J. Biol. Chem.  2007  282:24175-84  PubMed ID: 17584746

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