Cell No. : Cell Name
RCB0477 : NB9
update : 2023/09/12
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| Comment | Human cell line derived from Neuroblastoma. N-myc gene is amplified. Another name is CHP-212 (PMID: 6871857). Application consideration |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
Nishi, Yoshisuke
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| Originator |
Gilbert, F.
|
| Year of deposit |
1990
|
| Another name |
CHP-212
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Gender |
Male
|
| Age at sampling |
22 months
|
| Tissue |
nervous system
|
| Disease name |
neuroblastoma (stage IV)
|
| Classification |
cancer
|
| History |
Latt, S. A.
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|
Lifespan |
infinite
|
| Morphology |
other
|
| Cellosaurus(Expasy) |
CVCL_1125
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|
| |
deposit info |
lot info |
|
Medium |
Medium List |
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Culture type |
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Adherent/Suspension cells
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| Culture medium |
|
RPMI1640 + 15% FBS
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| Antibiotics |
|
Free
|
|
Passage method |
|
0.25% Trypsin (1min.) or pipetting
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Culture information
|
Passage ratio |
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1 : 8 split
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| SC frequency |
|
Subculture : once/week, Medium Renewal : 2 times/week
|
| Temperature |
|
37
℃
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| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
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| Freezing method |
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Slow freezing
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| Mycoplasma |
|
(-)
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| Virus (HIV) |
|
(-)
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| Isozyme |
|
LD, NP, AST, MPI
|
| Chromosome mode |
|
42-46(50) : 42(2),43(1),44(4),45(42),46(1)
|
| STR(human) |
|
OK
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
5
|
| User's Publication |
9
|
| Reference |
|
242
alent A, Le Roux G, Barrois M, Terrier-Lacombe MJ, Valteau-Couanet D, Léon B, Spengler B, Lenoir G, Bénard J, Bernheim A.
MYCN gene overrepresentation detected in primary neuroblastoma tumour cells without amplification
J Pathol
2002
198(4):495-501
PubMed ID: 12434419
DOI: 10.1002/path.1244
|
|
245
Kanda N, Tsuchida Y, Hata J, Kohl NE, Alt FW, Latt SA, Utakoji T.
Amplification of IMR-32 clones 8, G21, and N-myc in human neuroblastoma xenografts
Cancer Res
1987
47(12):3291-5
PubMed ID: 3034408
|
|
1287
Gilbert F, Feder M, Balaban G, Brangman D, Lurie, D K, Podolsky R, Rinaldt V, Vinikoor N, Weisband J
Human neuroblastomas and abnormalities of chromosomes 1 and 17.
Cancer Res
1984
44:5444-9
PubMed ID: 6488196
|
|
1286
U V Santer, M C Glick
Presence of fucosyl residues on the oligosaccharide antennae of membrane glycopeptides of human neuroblastoma cells
Cancer Res
1983
43(9):4159-66
PubMed ID: 6871857
|
|
1127
Gilbert F, Balaban G, Moorhead P, Bianchi D, Schlesinger H
Abnormalities of chromosome 1p in human neuroblastoma tumors and cell lines.
Cancer Genet Cytogenet
1982
7:33-42
PubMed ID: 7139592
DOI: 10.1016/0165-4608(82)90105-4
|
| User's Publication |
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17605
Hidaka A, Uekusa S, Hosokawa T, Kaneda H, Kazama T, Hagikura K, Uehara S, Koshinaga T, Matsumoto T.
Effects of dedifferentiated fat cells on neurogenic differentiation and cell proliferation in neuroblastoma cells
Pediatr Surg Int
2022
39(1):58
PubMed ID: 36547710
DOI: 10.1007/s00383-022-05304-x
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|
13941
Nagasaki-Maeoka E, Ikeda K, Takayama KI, Hirano T, Ishizuka Y, Koshinaga T, Tsukune N, Takayama T, Inoue S, Fujiwara K.
Polyethylene glycol derivative 9bw suppresses growth of neuroblastoma cells by inhibiting oxidative phosphorylation
Cancer Sci
2020
111(8):2943-2953
PubMed ID: 32495467
DOI: 10.1111/cas.14512
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4310
Hayashi H, Kubo Y, Izumida M, Takahashi E, Kido H, Sato K, Yamaya M, Nishimura H, Nakayama K, Matsuyama T.
Enterokinase Enhances Influenza A Virus Infection by Activating Trypsinogen in Human Cell Lines.
Front Cell Infect Microbiol
2018
PubMed ID: 29629340
DOI: 10.3389/fcimb.2018.00091
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10457
Watanabe Y, Ishizuka Y, Hirano T, Nagasaki-Maeoka E, Hoshi R, Yoshizawa S, Uekusa S, Kawashima H, Sugito K, Shinohara K, Fukuda N, Nagase H, Soma M, Koshinaga T, Fujiwara K.
ZAR1 knockdown promotes the differentiation of human neuroblastoma cells by suppression of MYCN expression.
Med. Oncol.
2017
34:158
PubMed ID: 28791558
DOI: 10.1007/s12032-017-0999-x
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14441
Hoshi R, Watanabe Y, Ishizuka Y, Hirano T, Nagasaki-Maeoka E, Yoshizawa S, Uekusa S, Kawashima H, Ohashi K, Sugito K, Fukuda N, Nagase H, Soma M, Ozaki T, Koshinaga T, Fujiwara K.
Depletion of TFAP2E attenuates adriamycin-mediated apoptosis in human neuroblastoma cells
Oncol Rep
2017
37(4):2459-2464
PubMed ID: 28260105
DOI: 10.3892/or.2017.5477
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14739
Takagi M, Yoshida M, Nemoto Y, Tamaichi H, Tsuchida R, Seki M, Uryu K, Nishii R, Miyamoto S, Saito M, Hanada R, Kaneko H, Miyano S, Kataoka K, Yoshida K, Ohira M, Hayashi Y, Nakagawara A, Ogawa S, Mizutani S, Takita J.
Loss of DNA Damage Response in Neuroblastoma and Utility of a PARP Inhibitor
J Natl Cancer Inst
2017
109(11)
PubMed ID: 29059438
DOI: 10.1093/jnci/djx062
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18785
Sugito K, Kawashima H, Yoshizawa S, Uekusa S, Hoshi R, Furuya T, Kaneda H, Hosoda T, Konuma N, Masuko T, Ohashi K, Ikeda T, Koshinaga T, Tomita R, Shinojima Y, Fujiwara K, Watanabe T, Held WA, Nagase H.
Non-promoter DNA hypermethylation of Zygote Arrest 1 (ZAR1) in neuroblastomas
J Pediatr Surg
2013
48(4):782-8
PubMed ID: 23583134
DOI: 10.1016/j.jpedsurg.2012.08.008
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18782
Kawashima H, Sugito K, Yoshizawa S, Uekusa S, Furuya T, Ikeda T, Koshinaga T, Shinojima Y, Hasegawa R, Mishra R, Igarashi J, Kimura M, Wang X, Fujiwara K, Gosh S, Nagase H.
DNA hypomethylation at the ZNF206-exon 5 CpG island associated with neuronal differentiation in mice and development of neuroblastoma in humans
Int J Oncol
2012
40(1):31-9
PubMed ID: 22011711
DOI: 10.3892/ijo.2011.1234
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18109
Maeda T, Nishimura N, Nakamura H, Sano K.
p21 (WAF1/Cip1/Sdi1/Pic1) mRNA is expressed in neuroblastoma cell lines but not in Ewing's sarcoma and primitive neuroectodermal tumor cell lines
Acta Paediatr Jpn
1997
39(5):590-4
PubMed ID: 9363658
DOI: 10.1111/j.1442-200x.1997.tb03646.x
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