Cell No. : Cell Name
RCB0475 : ILT-Mat
update : 2024/01/17
|
| Comment | IL-2 dependent ATL cell line. Based on the information by depositor; (1) this cell line possesses HTLV-1 pro-virus DNA, (2) the HTLV-1 proteins (p19, p24, p53, gp46) cannot be detected with western blotting, (3) it is expressing CD2, CD4, Ia, and IL-2R, but is not expressing CD3 or CD8. |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
Cell line to be handled at BSL 2(C-0070.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
|
|
|
Basic information
|
Depositor |
Sugamura, Kazuo
|
| Originator |
Sugamura, Kazuo
|
| Year of deposit |
1990
|
| Original cell |
TKG0286
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Race |
Japanese
|
| Gender |
Male
|
| Tissue |
blood
|
| Disease name |
ATL (acute T cell leukemia)
|
| Classification |
cancer
|
| History |
Decontaminated myco. by originator
|
| Memo_1 |
Mycoplasma was eliminated with MC-210 by the depositor.
|
|
Lifespan |
infinite
|
| Morphology |
lymphocyte-like
|
| BSL |
BSL2
|
| Cellosaurus(Expasy) |
CVCL_A782
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Suspension cells
|
| Culture medium |
|
RPMI1640 + 10% FBS + 200U/ml human IL-2
|
| Culture method |
|
|
| Antibiotics |
|
Free
|
|
Passage method |
|
dilution
|
Culture information
|
Passage ratio |
|
1 : 10 split
|
| SC frequency |
|
Subculture : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma |
|
(-)
|
| Virus (HIV) |
|
(-)
|
| Virus (HTLV-1) |
|
DNA (+)
|
| STR(human) |
|
OK
|
| Reference information |
Reference |
9
|
| User's Publication |
3
|
| Reference |
|
862
Chen S, Ishii N, Ine S, Ikeda S, Fujimura T, Ndhlovu LC, Soroosh P, Tada K, Harigae H, Kameoka J, Kasai N, Sasaki T, Sugamura K.
Regulatory T cell-like activity of Foxp3+ adult T cell leukemia cells
Int Immunol
2006
18(2):269-77
PubMed ID: 16361311
DOI: 10.1093/intimm/dxh366
|
|
228
Takeshita T, Arita T, Higuchi M, Asao H, Endo K, Kuroda H, Tanaka N, Murata K, Ishii N, Sugamura K.
STAM, signal transducing adaptor molecule, is associated with Janus kinases and involved in signaling for cell growth and c-myc induction
Immunity
1997
6(4):449-57.
PubMed ID: 9133424
DOI: 10.1016/s1074-7613(00)80288-5
|
|
234
Imada K, Takaori-Kondo A, Akagi T, Shimotohno K, Sugamura K, Hattori T, Yamabe H, Okuma M, Uchiyama T.
Tumorigenicity of human T-cell leukemia virus type I-infected cell lines in severe combined immunodeficient mice and characterization of the cells proliferating in vivo
Blood
1995
86(6):2350-7
PubMed ID: 7662981
|
|
229
Tanaka N, Asao H, Ohbo K, Ishii N, Takeshita T, Nakamura M, Sasaki H, Sugamura K.
Physical association of JAK1 and JAK2 tyrosine kinases with the interleukin 2 receptor beta and gamma chains
Proc Natl Acad Sci U S A
1994
91(15):7271-5
PubMed ID: 8041779
DOI: 10.1073/pnas.91.15.7271
|
|
232
Tanaka N, Asao H, Ohtani K, Nakamura M, Sugamura K.
A novel human tyrosine kinase gene inducible in T cells by interleukin 2
FEBS Lett
1993
324(1):1-5
PubMed ID: 8504851
DOI: 10.1016/0014-5793(93)81520-a
|
|
233
Takeshita T, Ohbo K, Nakamura M, Goto Y, Sugamura K.
Establishment of a cyclic adenosine monophosphate-dependent growing human T-cell line derived from an interleukin-2-dependent cell line
J Cell Physiol
1990
145(2):238-43
PubMed ID: 2174062
DOI: 10.1002/jcp.1041450207
|
|
1115
Takeshita T, Goto Y, Tada K, Nagata K, Asao H, Sugamura K
Monoclonal antibody defining a molecule possibly identical to the p75 subunit of interleukin 2 receptor.
J Exp Med
1989
169:1323-32
PubMed ID: 2784485
DOI: 10.1084/jem.169.4.1323
|
|
231
Takeshita T, Goto Y, Nakamura M, Fujii M, Iwami M, Hinuma Y, Sugamura K.
Phorbol esters can persistently replace interleukin-2 (IL-2) for the growth of a human IL-2-dependent T-cell line
J Cell Physiol
1988
136(2):319-25
PubMed ID: 3261728
DOI: 10.1002/jcp.1041360215
|
|
230
Ishii T, Kohno M, Nakamura M, Hinuma Y, Sugamura K.
Characterization of interleukin 2-stimulated phosphorylation of 67 and 63 kDa proteins in human T-cells
Biochem J
1987
242(1):211-9
PubMed ID: 3496080
DOI: 10.1042/bj2420211
|
| User's Publication |
|
13937
Mitoma S, El-Khaiat HM, Uto T, Sato K, Sekiguchi S, Norimine J.
Characterization of bovine interleukin-2 stably expressed in HEK-293 cells
J Vet Med Sci
2021
83(1):134-141
PubMed ID: 33177289
DOI: 10.1292/jvms.20-0423
|
|
10693
Tatsuro Watanabe, Satoshi Yamashita, Hiroshi Ureshino, Kazuharu Kamachi, Yuki Kurahashi, Yuki Fukuda-Kurahashi, Nao Yoshida, Naoko Hattori, Hideaki Nakamura, Akemi Sato, Atsushi Kawaguchi, Naoko Sueoka-Aragane, Kensuke Kojima, Seiji Okada, Toshikazu Ushijima, Shinya Kimura, Eisaburo Sueoka
Targeting Aberrant DNA Hypermethylation as a Driver of ATL Leukemogenesis Using the New Oral Demethylating Agent OR-2100
Blood
2020
136(7):871-884
PubMed ID: 32391874
DOI: 10.1182/blood.2019003084
|
|
2639
Oka, Takashi, Ouchida, Mamoru, Koyama, Maho, Ogama, Yoichiro, Takada, Shinichi, Nakatani, Yoko, Tanaka, Takehiro, Yoshino, Tadashi, Hayashi, Kazuhiko, Ohara, Nobuya, Kondo, Eisaku, Takahashi, Kiyoshi, Tsuchiyama, Junjiro, Tanimoto, Mitsune, Shimizu, Kenji, Akagi, Tadaatsu
Gene silencing of the tyrosine phosphatase SHP1 gene by aberrant methylation in leukemias/lymphomas.
Cancer Res
2002
62:6390-4
PubMed ID: 12438221
|
Back
Top
CELL BANK Website