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RCB0474 : K562/MTX-2  update : 2018/07/06
CommentMethotrexate(MTX)-resistant K562 cell line
Comment from the depositor
Terms and conditionsA prior written permission of the approver(Depositor) is necessary for any kind of use including academic use and for-profit use.
Remarks
approver's address
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English
Address
Kanazawa University
Graduate School of Medical Science Angiogenesis and Vascular Development (Department of Pediatrics)
13-1 Takaramachi, Kanazawa, Ishikawa 920-8640 ,Japan
Dr.Koizumi, Shoichi
Fax. +81-762-62-1866
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Koizumi, Shoichi
Originator Koizumi, Shoichi
Year of deposit 1990
Original cell K562
Animal human < Mammals
Genus Homo
Species sapiens
Gender Female
Tissue pleural effusion
Classification cancer
Memo_1 Mycoplasma was eliminated with MC-210.
Lifespan infinite
Morphology lymphocyte-like
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 10% FBS + 2x10^(-6)M MTX
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR (human) OK
Virus (HIV) (-)
Virus (HTLV-1) (-)
Isozyme LD, NP
Chromosome mode 44-61(50) : 55(2),56(3),57(19),58(23),59(2),61(1)
Reference information Reference 5
User's Publication 0


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Reference
1116  Impairment of Methotrexate(MTX)-Polyglutamate Formation of MTX-resistant K562 Cell Lines.  Koizumi S  Jpn J Cancer Res  1988  79:1230-1237  PubMed ID: 2465288
4602  Human chronic myelogenous leukemia cell-line with positive Philadelphia chromosome.  Lozzio CB, Lozzio BB.  Blood  1975  45(3):321-34  PubMed ID: 163658
4603  Properties and usefulness of the original K-562 human myelogenous leukemia cell line.  Lozzio BB, Lozzio CB.  Leuk Res  1979  3(6):363-70  PubMed ID: 95026
969  K562 human leukaemic cells synthesise embryonic haemoglobin in response to haemin.  Rutherford TR, Clegg JB, Weatherall DJ.  Nature  1979  280(5718):164-5  PubMed ID: 95354
970  Induction of erythroid differentiation in the human leukaemia cell line K562.  Andersson LC, Jokinen M, Gahmberg CG.  Nature  1979  278(5702):364-5  PubMed ID: 570644

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