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Cell No. : Cell Name
RCB0453 : SHOK  update : 2022/03/15
CommentContact-inhibited hamster embryonal cell line
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Miyoshi, Jun
Originator Azuma, T. & Suzuki, F.
Year of deposit 1989
Another name GHE-c8
Animal _hamster < Mammals
Strain name Syrian
Genus Mesocricetus
Species auratus
Age at sampling embryo
Tissue embryo/fetus, whole
Classification semi-normal
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_3164
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP, MPI, PepB
Chromosome mode 37-94(50) : /44(6),45(2),46(7),47(21),48(2),58(1)/
Images
deposit info
lot info
Reference information Reference 9
User's Publication 0


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Reference
211  Suzuki K, Miyoshi J, Watanabe M.  Function-dependent cooperation between oncogene activation and nonrandom chromosome change during tumorigenic conversion of Syrian hamster cells  Cancer Genet Cytogenet  1994  75(1):51-9  PubMed ID: 8039164   DOI: 10.1016/0165-4608(94)90215-1
210  Sasai H, Higashi T, Nakamori S, Miyoshi J, Suzuki F, Nomura T, Kakunaga T.  Syrian hamster embryo cell lines useful for detecting transforming genes in mouse tumours: detection of transforming genes in X-ray-related mouse tumours  Br J Cancer  1993  67(2):262-7  PubMed ID: 8431357   DOI: 10.1038/bjc.1993.50
205  Suzuki K, Watanabe M, Miyoshi J.  Differences in effects of oncogenes on resistance of gamma rays, ultraviolet light, and heat shock  Radiat Res  1992  129(2):157-62  PubMed ID: 1734445  
208  Ohuchi T, Kurita Y, Sasai H, Miyoshi J, Nomura T, Toyoshima K.  Oncogenic activation of murine mos protein kinase by DNA rearrangement of its N-terminal coding region  Oncogene  1992  7(2):331-8  PubMed ID: 1532243  
203  Miyoshi J, Higashi T, Mukai H, Ohuchi T, Kakunaga T.  Structure and transforming potential of the human cot oncogene encoding a putative protein kinase  Mol Cell Biol  1991  11(8):4088-96  PubMed ID: 2072910   DOI: 10.1128/mcb.11.8.4088-4096.1991
209  Aoki M, Akiyama T, Miyoshi J, Toyoshima K.  Identification and characterization of protein products of the cot oncogene with serine kinase activity  Oncogene  1991  6(9):1515-9  PubMed ID: 1833717  
212  Orita S, Higashi T, Kawasaki Y, Harada A, Igarashi H, Monden T, Morimoto H, Shimano T, Mori T, Miyoshi J.  A novel point mutation at codon 146 of the K-ras gene in a human colorectal cancer identified by the polymerase chain reaction  Virus Genes  1991  5(1):75-9  PubMed ID: 2017878   DOI: 10.1007/BF00571733
204  Higashi T, Sasai H, Suzuki F, Miyoshi J, Ohuchi T, Takai S, Mori T, Kakunaga T.  Hamster cell line suitable for transfection assay of transforming genes  Proc Natl Acad Sci U S A  1990  87(7):2409-13  PubMed ID: 2181436   DOI: 10.1073/pnas.87.7.2409
213  Nakamori S, Mukai H, Higashi T, Sasai H, Miyoshi J, Takai S, Mori T, Kakunaga T.  A simple and useful method for simultaneous screening of elevated levels of expression of a variety of oncogenes in malignant cells  Jpn J Cancer Res  1988  79(12):1311-7.  PubMed ID: 3148601   DOI: 10.1111/j.1349-7006.1988.tb01561.x

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