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RCB0441 : SCCKN  update : 2018/07/06
CommentHighly sensitive to bleomycin
Comment from the depositor
Restriction (Category)a
Terms and conditionsBasically, there is no restriction regarding academic use.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Urade, Masahiro
Originator Urade, M. & Mima, T.
Year of deposit 1989
Animal human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Male
Age at sampling 75 years
Tissue tongue
Case history squamous carcinoma
Metastastatic ability Yes
Metastatsd tissue subcutaneous tissue
Classification cancer
Year of origin 1987
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR (human) OK
Virus (HIV) (-)
Isozyme LD, NP
Chromosome mode 51-136(50) : /66(4),67(2),68(6),69(6),70(8),71(6)/
Images
deposit info
lot info
Reference information Reference 2
User's Publication 8


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Reference
Urade, M., Ogura, T., Mima, T., Matsuya, T.  Acquisition of bleomycin (BLM) resistance in human squamous cell carcinoma transplantable in nude mice  The Cancer Journal  1990  3:192-196 
Urade M, Ogura T, Mima T, Matsuya T  Establishment of human squamous carcinoma cell lines highly and minimally sensitive to bleomycin and analysis of factors involved in the sensitivity.  Cancer  1992  69:2589-97 

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User's Publication
Kuratare, I. et al  Cell death induction of thymidine kinase gene transfer followed by ganciclovir treatment in oral squamous cell carcinoma cell lines  Yonago Acta medica   2005  48:63-74 
Nishimaki, Haruaki, Kasai, Kenji, Kozaki, Ken ichi, Takeo, Tomohiro, Ikeda, Hiroshi, Saga, Shinsuke, Nitta, Masakazu, Itoh, Gen  A role of activated Sonic hedgehog signaling for the cellular proliferation of oral squamous cell carcinoma cell line.  Biochem Biophys Res Commun  2004  314:313-20  PubMed ID: 14733907
Miyazawa, Jun, Mitoro, Akira, Kawashiri, Shuichi, Chada, Kiran K, Imai, Kazushi  Expression of mesenchyme-specific gene HMGA2 in squamous cell carcinomas of the oral cavity.  Cancer Res  2004  64:2024-9  PubMed ID: 15026339
Okamura, Hirohiko, Yoshida, Kaya, Morimoto, Hiroyuki, Haneji, Tatsuji  PTEN expression elicited by EGR-1 transcription factor in calyculin A-induced apoptotic cells.  J Cell Biochem  2005  94:117-25  PubMed ID: 15517593
Shibata, Masami, Kodani, Isamu, Osaki, Mitsuhiko, Araki, Kunio, Adachi, Hironobu, Ryoke, Kazuo, Ito, Hisao  Cyclo-oxygenase-1 and -2 expression in human oral mucosa, dysplasias and squamous cell carcinomas and their pathological significance.  Oral Oncol  2005  41:304-12  PubMed ID: 15743693
Maemoto S, Yumoto M, Ibata M, Torizuka S, Ozawa N, Tatsumi S, Hashido M, Morikawa M, Maeda G, Imai K.  Mutational analysis of HRAS and KRAS genes in oral carcinoma cell lines.  Odontology  2011  100:149-55  PubMed ID: 21607592
Kuratare I. et al.  Cell death induction of thymidine kinase gene transfer followed by ganciclovir treatment in oral squamous cell carcinoma cell lines.  Yonago Acta Med.  2005  48:63-74 
Oka T, Mizuno H, Sakata M, Fujita H, Yoshino T, Yamano Y, Utsumi K, Masujima T, Utsunomiya A.  Metabolic abnormalities in adult T-cell leukemia/lymphoma and induction of specific leukemic cell death using photodynamic therapy.  Sci Rep  2018    PubMed ID: 30297858



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