Cell No. : Cell Name RCB0439 : LC-1F  update : 2024/12/05
Comment			Human cell line derived from lung cancer. Squamous cell carcinoma. Floating cells (non-adherent cells). LC-1/sq-SF (RCB0438), LC-1F (RCB0439), and LC-1/sq (RCB0455) are derived from the same patient.
Comment from the depositor
Terms and conditions			There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	Itoh, Hiroshi
		Originator	Kataoka, Hiroaki
		Year of deposit	1989
		Original cell	LC-1/sq
		Animal	_human < Mammals
		Genus	Homo
		Species	sapiens
		Race	Japanese
		Gender	Male
		Age at sampling	69 years
		Tissue	lung
		Classification	cancer
		Year of origin	1986
		History	H. Kataoka
		Memo_1	Mycoplasma was eliminated with MC-210.
		Lifespan	infinite
		Morphology	other
		Cellosaurus(Expasy)	CVCL_1372
			deposit info	lot info
		Medium	Medium List
		Culture type		Suspension cells
		Culture method		General Guidelines for Culturing Suspension Cells, 浮遊細胞の培養に関する一般的な注意(Japanese)
		Culture medium		(HamF12 : RPMI1640 = 1 : 1) + 25mM HEPES + 10% FBS
		Antibiotics		Free
		Passage method		dilution
	Culture information	Passage ratio		1 : 4 split
		SC frequency		Subculture : once/week, Medium Renewal : 2 times/week
		Temperature		37 ℃
		CO2 concentration		5 %
		Freeze medium		Medium + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma/Acholeplasma		(-)
		Virus (HIV)		(-)
		Isozyme		LD, NP
		Chromosome mode		73-181(50) : /83(5),84(1),85(11),86(5),87(3),88(2)/
		STR(human)		OK
Images			deposit info	lot info
Reference information		Reference	1
		User's Publication	9

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Reference

1779  Itoh H, Kataoka H, Koita H, Nabeshima K, Inoue T, Kangawa K, Koono M  Establishment of a new human cancer cell line secreting protease nexin-II/amyloid beta protein precursor derived from squamous-cell carcinoma of lung.  Int J Cancer  1991  49:436-43  PubMed ID: 1917142   DOI: 10.1002/ijc.2910490322

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User's Publication

13332  Hase H, Aoki M, Matsumoto K, Nakai S, Nagata T, Takeda A, Ueda K, Minami K, Kitae K, Jingushi K, Ueda Y, Yamamoto M, Furukawa T, Sato M, Tsujikawa K.  Cancer type‑SLCO1B3 promotes epithelial‑mesenchymal transition resulting in the tumour progression of non‑small cell lung cancer.  Oncol Rep  2021    PubMed ID: 33155667   DOI: 10.3892/or.2020.7839

13773  Saigusa D, Motoike IN, Saito S, Zorzi M, Aoki Y, Kitamura H, Suzuki M, Katsuoka F, Ishii H, Kinoshita K, Motohashi H, Yamamoto M.  Impacts of NRF2 activation in non-small-cell lung cancer cell lines on extracellular metabolites  Cancer Sci  2020  111(2):667-678  PubMed ID: 31828882   DOI: 10.1111/cas.14278

19029  Kanamori-Katayama M, Kaiho A, Ishizu Y, Okamura-Oho Y, Hino O, Abe M, Kishimoto T, Sekihara H, Nakamura Y, Suzuki H, Forrest AR, Hayashizaki Y.  LRRN4 and UPK3B are markers of primary mesothelial cells  PLoS One  2011  6(10):e25391  PubMed ID: 21984916   DOI: 10.1371/journal.pone.0025391

6410  Danjoh I, Sone H, Noda N, Iimura E, Nagayoshi M, Saijo K, Hiroyama T, Nakamura Y.  Is parainfluenza virus a threatening virus for human cancer cell lines?  Hum Cell  2009  22(3):81-4  PubMed ID: 19624309   DOI: 10.1111/j.1749-0774.2009.00071.x

18072  Cheng CM, Tsuneyama K, Matsui K, Takahashi H, Ishizawa S, Takano Y.  Cytoplasmic expression of c-erbB2 in non-small cell lung cancers  Virchows Arch  2005  446(6):596-603  PubMed ID: 15902485   DOI: 10.1007/s00428-005-1258-7

16489  Seike M, Kondo T, Fujii K, Okano T, Yamada T, Matsuno Y, Gemma A, Kudoh S, Hirohashi S.  Proteomic signatures for histological types of lung cancer  Proteomics  2005  5(11):2939-48  PubMed ID: 15996008   DOI: 10.1002/pmic.200401166

3338  Seike, Masahiro, Kondo, Tadashi, Fujii, Kazuyasu, Yamada, Tesshi, Gemma, Akihiko, Kudoh, Shoji, Hirohashi, Setsuo  Proteomic signature of human cancer cells.  Proteomics  2004  4:2776-88  PubMed ID: 15352251   DOI: 10.1002/pmic.200300795

16491  Nakanishi H, Suda T, Katoh M, Watanabe A, Igishi T, Kodani M, Matsumoto S, Nakamoto M, Shigeoka Y, Okabe T, Oshimura M, Shimizu E.  Loss of imprinting of PEG1/MEST in lung cancer cell lines  Oncol Rep  2004  12(6):1273-8  PubMed ID: 15547750

3134  Yoshimasu, T. et al  Integrins α2β1,α5β1 and αvβ5 are related to tumor growth and metastasis of non-small cell cancer   Japanese Journal of Lung Cancer  2001  41:111-115    DOI: 10.2482/haigan.41.111