Search
CELL BANK Website

Back Back
RCB0436 : HUC-F2  update : 2018/07/06
CommentNormal umbilical card fibroblast, female
Comment from the depositor
Terms and conditionsBasically, there is no restriction regarding academic use.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Nishida, M.& Ohno, T.
Originator Ohno, Tadao
Year of deposit 1989
Animal human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Female
Tissue umbilical cord
Classification normal
Year of origin 1989
Lifespan finite
Morphology fibroblast-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives MEM ALPHA + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR (human) OK
Animal PCR OK
Virus (HIV) (-)
Isozyme LD, NP
Chromosome mode 44-72(50) : 44(4),45(9),46(36),72(1)
Images
deposit info
lot info
Reference information Reference 0
User's Publication 8


To topTop
Reference

To topTop
User's Publication
2723  Ohshima S  Apoptosis in stress-induced and spontaneously senescent human fibroblasts.  Biochem Biophys Res Commun  2004  324:241-6  PubMed ID: 15465009
2824  Kakeda M, Hiratsuka M, Nagata K, Kuroiwa Y, Kakitani M, Katoh M, Oshimura M, Tomizuka K  Human artificial chromosome (HAC) vector provides long-term therapeutic transgene expression in normal human primary fibroblasts.  Gene Ther  2005  12:852-6  PubMed ID: 15750614
7604  Yamashita S, Ogawa K, Ikei T, Udono M, Fujiki T, Katakura Y.  SIRT1 prevents replicative senescence of normal human umbilical cord fibroblast through potentiating the transcription of human telomerase reverse transcriptase gene.  Biochem. Biophys. Res. Commun.  2011  417:630-4  PubMed ID: 22197555
7706  Atsumi Y, Fujimori H, Fukuda H, Inase A, Shinohe K, Yoshioka Y, Shikanai M, Ichijima Y, Unno J, Mizutani S, Tsuchiya N, Hippo Y, Nakagama H, Masutani M, Teraoka H, Yoshioka K.  Onset of quiescence following p53 mediated down-regulation of H2AX in normal cells.  PLoS ONE  2011  6:e23432  PubMed ID: 21858116
8788  Hiroyama T, Sudo K, Aoki N, Miharada K, Danjo I, Fujioka T, Nagasawa T, Nakamura Y.  Human umbilical cord-derived cells can often serve as feeder cells to maintain primate embryonic stem cells in a state capable of producing hematopoietic cells.  Cell Biol. Int.  2007  32:1-7  PubMed ID: 17890111
5393  Harada M, Imai J, Okuno S, Suzuki T.  Macrophage-mediated activation of camptothecin analogue T-2513-carboxymethyl dextran conjugate (T-0128): possible cellular mechanism for antitumor activity.  J Control Release  2000  69(3):389-97  PubMed ID: 11102679
5396  Hosomi O, Misawa Y, Takeya A, Matahira Y, Sugahara K, Kubohara Y, Yamakura F, Kudo S.  Novel oligosaccharide has suppressive activity against human leukemia cell proliferation.  Glycoconj J  2009  26(2):189-98  PubMed ID: 18726690
3579  Sudo K, Kanno M, Miharada K, Ogawa S, Hiroyama T, Saijo K, Nakamura Y.  Mesenchymal progenitors able to differentiate into osteogenic, chondrogenic, and/or adipogenic cells in vitro are present in most primary fibroblast-like cell populations.  Stem Cells  2007  25(7):1610-7  PubMed ID: 17395773



Back Back Return Top Page