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RCB0426 : SK-N-SH  update : 2018/07/06
CommentNeuroblastoma, a target in CTL assay
Comment from the depositor
Terms and conditionsResearch purpose only. Send APPROVAL Form(C-0006) directly to RCB.
approver's address
Contact Us
RIKEN, BioResource Research Center
Cell Engineering Division
Dr.Cell Bank
Fax. +81-29-836-9130
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.
Basic information Depositor RCB (Noda, Makoto)
Originator Biedler, J. L.
Year of deposit 1989
Animal human < Mammals
Genus Homo
Species sapiens
Gender Female
Age at sampling 4 years
Tissue neuroblastoma
Case history neuroblastoma
Metastastatic ability Yes
Classification cancer
Year of origin 1970
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives MEM ALPHA + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR (human) OK
Virus (HIV) (-)
Isozyme LD, NP
Chromosome mode 46-48(50) : 46(1),47(48),48(1)
deposit info
lot info
Reference information Reference 11
User's Publication 35

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1169  Bluestein, H G  Neurocytotoxic antibodies in serum of patients with systemic lupus erythematosus.  Proc Natl Acad Sci U S A  1978  75:3965-9  PubMed ID: 279013
1122  Seeger, R C, Rayner, S A, Banerjee A, Chung H, Laug, W E, Neustein, H B, Benedict, W F  Morphology, growth, chromosomal pattern and fibrinolytic activity of two new human neuroblastoma cell lines.  Cancer Res  1977  37:1364-71  PubMed ID: 856461
1287  Gilbert F, Feder M, Balaban G, Brangman D, Lurie, D K, Podolsky R, Rinaldt V, Vinikoor N, Weisband J  Human neuroblastomas and abnormalities of chromosomes 1 and 17.  Cancer Res  1984  44:5444-9  PubMed ID: 6488196
4576  Biedler JL, Helson L, Spengler BA.  Morphology and growth, tumorigenicity, and cytogenetics of human neuroblastoma cells in continuous culture.  Cancer Res  1973  33(11):2643-52  PubMed ID: 4748425
4673  Biedler JL, Spengler BA.  A novel chromosome abnormality in human neuroblastoma and antifolate-resistant Chinese hamster cell lives in culture.  J Natl Cancer Inst  1976  57(3):683-95  PubMed ID: 62055
4674  Barnes EN, Biedler JL, Spengler BA, Lyser KM.  The fine structure of continuous human neuroblastoma lines SK-N-SH, SK-N-BE(2), and SK-N-MC.  In Vitro  1981  17(7):619-31  PubMed ID: 7327593
4675  Ross RA, Spengler BA, Biedler JL.  Coordinate morphological and biochemical interconversion of human neuroblastoma cells.  J Natl Cancer Inst  1983  71(4):741-7  PubMed ID: 6137586
4676  Spengler BA, Ross RA, Biedler JL.  Differential drug sensitivity of human neuroblastoma cells.  Cancer Treat Rep  1986  70(8):959-65  PubMed ID: 3731152
4693  Casper JT, Borella L, Sen L.  Reactivity of human brain antiserum with neuroblastoma cells and nonreactivity with thymocytes and lymphoblasts.  Cancer Res  1977  37(6):1750-6  PubMed ID: 66986
4694  West GJ, Uki J, Herschman HR, Seeger RC.  Adrenergic, cholinergic, and inactive human neuroblastoma cell lines with the action-potential Na+ ionophore.  Cancer Res  1977  37(5):1372-6  PubMed ID: 15722
4695  Danon YL, Epstein MB, Siegel MM, Rucker N, Myers CP, Norman A, Benedict WF, Seeger RC.  Variability in DNA distributions of human neuroblastomas after cyclophosphamide.  Cancer Treat Rep  1980  64(12):1275-81  PubMed ID: 7471116

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User's Publication
2895  Motegi, Akira, Fujimoto, Jiro, Kotani, Masaharu, Sakuraba, Hitoshi, Yamamoto, Tadashi  ALK receptor tyrosine kinase promotes cell growth and neurite outgrowth.  J Cell Sci  2004  117:3319-29  PubMed ID: 15226403
2214  Katayama M, Iwamatsu A, Masutani H, Furuke K, Takeda K, Wada H, Masuda T, Ishii K  Expression of neural cell adhesion molecule L1 in human lung cancer cell lines.  Cell Struct Funct  1997  22:511-6  PubMed ID: 9431455
2358  Kimura M, Matsuda Y, Yoshioka T, Okano Y  Cell cycle-dependent expression and centrosome localization of a third human aurora/Ipl1-related protein kinase, AIK3.  J Biol Chem  1999  274:7334-40  PubMed ID: 10066797
2433  Tohda C, Jacobowitz, D M  The function and expression of sproutin, a novel neurite outgrowth factor.  Neuroreport  1999  10:2089-94  PubMed ID: 10424680
3119  Wasa M, Wang, H S, Tazuke Y, Okada A  Insulin-like growth factor-I stimulates amino acid transport in a glutamine-deprived human neuroblastoma cell line.  Biochim Biophys Acta  2001  1525:118-24  PubMed ID: 11342260
3133  Wang, H S, Wasa M, Okada A  Regulation of glutamine transport by the type I insulin-like growth factor receptor in a human neuroblastoma cell line.  Cancer Lett  2001  168:81-6  PubMed ID: 11368881
3122  Wasa, Masafumi, Wang, Hong-Sheng, Okada, Akira  Characterization of L-glutamine transport by a human neuroblastoma cell line.  Am J Physiol Cell Physiol  2002  282:C1246-53  PubMed ID: 11997238
3123  Wang, Hong-Sheng, Wasa, Masafumi, Okada, Akira  Amino acid transport in a human neuroblastoma cell line is regulated by the type I insulin-like growth factor receptor.  Life Sci  2002  71:127-37  PubMed ID: 12031683
3211  Mori, Kenji, Muto, Yoshinori, Kokuzawa, Jouji, Yoshioka, Takashi, Yoshimura, Shinichi, Iwama, Toru, Okano, Yukio, Sakai, Noboru  Neuronal protein NP25 interacts with F-actin.  Neurosci Res  2004  48:439-46  PubMed ID: 15041197
7835  Niitsu Y, Hakamata M, Goto Y, Higashi T, Shoji M, Sugai T, Umezawa K.  Chemoenzymatic synthesis of (2R,3R,4R)-dehydroxymethylepoxyquinomicin (DHMEQ), a new activator of antioxidant transcription factor Nrf2.  Org. Biomol. Chem.  2011  9:4635-41  PubMed ID: 21541373
7941  Hayama T, Tabata K, Uchiyama T, Fujimoto Y, Suzuki T.  Ferrearin C induces apoptosis via heme oxygenase-1 (HO-1) induction in neuroblastoma.  J Nat Med  2011  65:431-9  PubMed ID: 21327520
8074  Hiratsuka T, Matsuzaki S, Miyata S, Kinoshita M, Kakehi K, Nishida S, Katayama T, Tohyama M.  Yokukansan inhibits neuronal death during ER stress by regulating the unfolded protein response.  PLoS ONE  2010  5:e13280  PubMed ID: 20967273
8096  Ishikawa S, Taira T, Takahashi-Niki K, Niki T, Ariga H, Iguchi-Ariga SM.  Human DJ-1-specific transcriptional activation of tyrosine hydroxylase gene.  J. Biol. Chem.  2010  285:39718-31  PubMed ID: 20938049
8129  Koyama Y, Hiratsuka T, Matsuzaki S, Yamagishi S, Kato S, Katayama T, Tohyama M.  Familial amyotrophic lateral sclerosis (FALS)-linked SOD1 mutation accelerates neuronal cell death by activating cleavage of caspase-4 under ER stress in an in vitro model of FALS.  Neurochem. Int.  2010  57:838-43  PubMed ID: 20816908
8144  Yaguchi T, Saito M, Yasuda Y, Kanno T, Nakano T, Nishizaki T.  Higher concentrations of extracellular ATP suppress proliferation of Caco-2 human colonic cancer cells via an unknown receptor involving PKC inhibition.  Cell. Physiol. Biochem.  2010  26:125-34  PubMed ID: 20798496
8367  Koshimizu TA, Fujiwara Y, Sakai N, Shibata K, Tsuchiya H.  Oxytocin stimulates expression of a noncoding RNA tumor marker in a human neuroblastoma cell line.  Life Sci.  2010  86:455-60  PubMed ID: 20149803
8382  Koshimizu TA, Tsuchiya H, Tsuda H, Fujiwara Y, Shibata K, Hirasawa A, Tsujimoto G, Fujimura A.  Inhibition of heat shock protein 90 attenuates adenylate cyclase sensitization after chronic morphine treatment.  Biochem. Biophys. Res. Commun.  2010  392:603-7  PubMed ID: 20100459
8388  Toyohira Y, Ueno S, Tsutsui M, Itoh H, Sakai N, Saito N, Takahashi K, Yanagihara N.  Stimulatory effects of the soy phytoestrogen genistein on noradrenaline transporter and serotonin transporter activity.  Mol Nutr Food Res  2010  54:516-24  PubMed ID: 20087855
8625  Futami H, Sakai R.  RET protein promotes non-adherent growth of NB-39-nu neuroblastoma cell line.  Cancer Sci.  2009  100:1034-9  PubMed ID: 19320641
9561  Anindita PD, Sasaki M, Nobori H, Sato A, Carr M, Ito N, Sugiyama M, Orba Y, Sawa H.  Generation of recombinant rabies viruses encoding NanoLuc luciferase for antiviral activity assays.  Virus Res.  2016  215:121-8  PubMed ID: 26869397
9712  Kurita M, Hanada S, Ichimaru Y, Saito H, Tabata K, Asami S, Miyairi S, Suzuki T.  Indirubin 3'-Epoxide Induces Caspase-Independent Cell Death in Human Neuroblastoma.  Biol. Pharm. Bull.  2016  39:993-9  PubMed ID: 27251501
9713  Sakamoto R, Kakinuma E, Masuda K, Takeuchi Y, Ito K, Iketaki K, Matsuzaki T, Nakabayashi S, Yoshikawa HY, Yamamoto H, Sato Y, Tanii T.  Quantitative comparison of cancer and normal cell adhesion using organosilane monolayer templates: an experimental study on the anti-adhesion effect of green-tea catechins.  In Vitro Cell. Dev. Biol. Anim.  2016  52:799-805  PubMed ID: 27251159
9924  Morikawa Y, Shibata A, Okumura N, Ikari A, Sasajima Y, Suenami K, Sato K, Takekoshi Y, El-Kabbani O, Matsunaga T.  Sibutramine provokes apoptosis of aortic endothelial cells through altered production of reactive oxygen and nitrogen species.  Toxicol. Appl. Pharmacol.  2017  314:1-11  PubMed ID: 27838152
10236  Sudo K, Niikura K, Iwaki K, Kohyama S, Fujiwara K, Doi N.  Human-derived fusogenic peptides for the intracellular delivery of proteins.  J Control Release  2017  255:1-11  PubMed ID: 28385674
10332  Matsunaga T, Morikawa Y, Kamata K, Shibata A, Miyazono H, Sasajima Y, Suenami K, Sato K, Takekoshi Y, Endo S, El-Kabbani O, Ikari A.  α-Pyrrolidinononanophenone provokes apoptosis of neuronal cells through alterations in antioxidant properties.  Toxicology  2017  386:93-102  PubMed ID: 28578026
10533  Matsunaga T, Morikawa Y, Kamase K, Horinouchi M, Sasajima Y, Suenami K, Sato K, Takekoshi Y, Endo S, El-Kabbani O, Ikari A.  Enhancement of Endothelial Barrier Permeability by Mitragynine.  Biol. Pharm. Bull.  2017  40:1779-1783  PubMed ID: 28966251
10598  Sakagami H, Suzuki R, Shirataki Y, Iwama S, Nakagawa M, Suzuki H, Tanaka K, Tamura N, Takeshima H.  Re-evaluation of Culture Condition of PC12 and SH-SY5Y Cells Based on Growth Rate and Amino Acid Consumption.  In Vivo  2017  31:1089-1095  PubMed ID: 29102930
10605  Matsunaga T, Kamase K, Takasawa H, Yamaji Y, Endo S, El-Kabbani O, Ikari A.  Facilitation of 9,10-phenanthrenequinone-elicited neuroblastoma cell apoptosis by NAD(P)H:quinone oxidoreductase 1.  Chem. Biol. Interact.  2018  279:10-20  PubMed ID: 29108775
10678  Shobatake R, Takasawa K, Ota H, Itaya-Hironaka A, Yamauchi A, Sakuramoto-Tsuchida S, Uchiyama T, Makino M, Sugie K, Takasawa S, Ueno S.  Up-regulation of POMC and CART mRNAs by intermittent hypoxia via GATA transcription factors in human neuronal cells.  Int. J. Biochem. Cell Biol.  2018  95:100-107  PubMed ID: 29275211
10728  Kurita M, Takada T, Wakabayashi N, Asami S, Ono S, Uchiyama T, Suzuki T.  Antitumor Effect of Burchellin Derivatives Against Neuroblastoma.  Anticancer Res.  2018  38:855-862  PubMed ID: 29374712
10765  Sakagami H, Tsuji M, Tomomura M, Masuda Y, Iwama S, Nakagawa M, Suzuki H, Tanaka K, Abe T, Tamura N, Tomomura A, Yokose S, Takeshima H, Natori T, Horiuchi M, Fujisawa T, Kiuchi Y, Oguchi K, Yasui T, Oizumi H, Oizumi T.  Protection of Differentiating Neuronal Cells from Amyloid β Peptide-induced Injury by Alkaline Extract of Leaves of Sasa senanensis Rehder.  In Vivo  2018  32:231-239  PubMed ID: 29475904
11049  Sasaki M, Anindita PD, Ito N, Sugiyama M, Carr M, Fukuhara H, Ose T, Maenaka K, Takada A, Hall WW, Orba Y, Sawa H  The Role of Heparan Sulfate Proteoglycans as an Attachment Factor for Rabies Virus Entry and Infection.  J Infect Dis  2018  217(11):1740-1749  PubMed ID: 29529215
5374  Jimbo T, Nakayama J, Akahane K, Fukuda M.  Effect of polysialic acid on the tumor xenografts implanted into nude mice.  Int J Cancer  2001  94(2):192-9  PubMed ID: 11668497
5410  Tohda C, Matsumoto N, Zou K, Meselhy MR, Komatsu K.  Axonal and dendritic extension by protopanaxadiol-type saponins from ginseng drugs in SK-N-SH cells.  Jpn J Pharmacol  2002  90(3):254-62  PubMed ID: 12499580
3585  Motoki T, Sugiura Y, Matsumoto Y, Tsuji T, Kubota S, Takigawa M, Gohda E.  Induction of hepatocyte growth factor expression by maleic acid in human fibroblasts through MAPK activation.  J Cell Biochem  2008  104(4):1465-76  PubMed ID: 18491380

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