Cell No. : Cell Name RCB0420 : BHK-21C13-2P  update : 2024/01/17
Comment			Culturable in suspension, cf. ECACC ( BHK-21C13-3P). Since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECACC 84111301 prior to order.
Comment from the depositor
Terms and conditions			1) There is no restriction regarding use for basic researches. However, since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECA84111301 prior to order. 2) In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	ECACC
		Originator	Radlett, P. & Draper, M.
		Year of deposit	1989
		Animal	_hamster < Mammals
		Strain name	Syrian
		Genus	Mesocricetus
		Species	auratus
		Tissue	kidney
		Classification	other
		History	overseas, ECACC
		Lifespan	infinite
		Morphology	lymphocyte-like
		Cellosaurus(Expasy)	CVCL_8837
			deposit info	lot info
		Medium	Medium List
		Culture type		Suspension cells
		Culture medium		MEM (suspension) + 10% FBS + 0.1mM NEAA
		Culture method
		Antibiotics		Free
		Passage method		dilution
	Culture information	Passage ratio		1 : 10 split
		SC frequency		Subculture : 2 times/week
		Temperature		37 ℃
		CO2 concentration		5 %
		Freeze medium		Medium + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma		(-)
		Animal PCR		OK
		Isozyme		LD, NP
Reference information		Reference	3
		User's Publication	2

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Reference

2078  Macpherson, I., A.  AGAR SUSPENSION CULTURE FOR THE SELECTIVE ASSAY OF CELLS TRANSFORMED BY POLYOMA VIRUS.  Virology  1964  23:291-294  PubMed ID: 14187925   DOI: 10.1016/0042-6822(64)90301-0

2077  Ian Macpherson  Characteristics of a Hamster Cell Clone Transformed by Polyoma Virus  J Natl Cancer Inst  1963  30:795-815    DOI: 10.1093/jnci/30.4.795

18  Macpherson,I. and Stoker,M.  Polyoma transformation of hamster cell clones----an investigation of genetic factors affecting cell competence.  Virology  1962  16:147-151  PubMed ID: 14468055   DOI: 10.1016/0042-6822(62)90290-8

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User's Publication

8312  Lee BH, Oyane A, Tsurushima H, Shimizu Y, Sasaki T, Koshizaki N.  A new approach for hydroxyapatite coating on polymeric materials using laser-induced precursor formation and subsequent aging.  ACS Appl Mater Interfaces  2009  1:1520-4  PubMed ID: 20355955   DOI: 10.1021/am900183e

18055  You J, Kamihira M, Iijima S.  Surfactant-mediated gene transfer for animal cells  Cytotechnology  1997  25(1-3):45-52  PubMed ID: 22358878   DOI: 10.1023/A:1007955631313