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Cell No. : Cell Name
RCB0371 : NG-3Y1-T18D  update : 2022/03/14
CommentA subline of 3Y1 cell line. Transformed by nitrosoguanidine.
Comment from the depositor
Terms and conditionsNo restriction, but required to cite ref. (Fukuoka Igaku Zasshi 1987;78:569-577) and to send a reprint to originator.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Kimura, Genki
Originator Kimura, Genki
Year of deposit 1989
Animal _rat < Mammals
Strain name Fischer
Gender Female
Age at sampling embryo
Tissue embryo/fetus, whole
Classification transformed
Memo_1 Mycoplasma was eliminated with MC-210.
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_H237
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP, MPI, PepB
Reference information Reference 1
User's Publication 0


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Reference
1052  Ohno K, Zaitsu H, Kimura G.  Maintenance of postconfluence stationary cell density by transient increase and decrease in cell number upon medium renewals in rat 3Y1 fibroblasts. Diminution of the decrease in cell number after cell transformation by N-methyl-N'-nitro-N-nitrosoguanidine.  Fukuoka Igaku Zasshi.  1987  78(12):569-77  PubMed ID: 3447927  

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