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RCB0285 : CHO-K1  update : 2018/07/06
特性(英)Widely used cell line, especially for biotechnol
特性(日) 遺伝子組換えによる物質生産に多用される細胞株。プロリン要求性である。
寄託者特性(英)
寄託者特性(日)
使用条件a
使用条件(英)Basically, there is no restriction regarding academic use.
使用条件(日)学術研究における利用につきましては原則として使用制限はありません。
提供申込書類 依頼書C-0001.pdf   同意書(非営利)C-0003.pdf   同意書(営利)C-0003p.pdf  
提供同意書は、使用機関及び使用内容に応じて二種類あります。該当する提供同意書をご使用ください(詳細)。特許等の取得及び商業利用等は別途ご相談(cellbank.brc@riken.jp)を承ります。
細胞基本情報 寄託者 Seto, Toshio
樹立者 Puck, T. T.
寄託日 1989
動物種 hamster < Mammals
動物系統名 Chinese
属名 Cricetulus
種名 griseus
性別 Female
adult
採取組織 ovary
細胞分類 other
細胞寿命 infinite
細胞形態 epithelial-like
細胞培養情報
寄託時情報
ロット情報
培地・試薬情報 培地・試薬一覧はこちらをご覧ください。
培養形態 Adherent cells
培地 HamF12 + 10% FBS
抗生物質 Free
継代方法 0.25% trypsin
継代密度 1 : 8 split
継代または培地交換頻度 Subculture : 2 times/week
培養最適温度 37 ℃
二酸化炭素濃度 5 %
凍結培地 Medium + 10% DMSO
凍結方法 Slow freezing
マイコプラズマ (-)
動物種PCR OK
染色体検査 17-58(50) : 17(3),18(3),19(10),20(21),21(6),26(1)/
画像情報
寄託時情報
ロット情報
文献情報 Reference(英) 2件
Reference(日) 1件
利用者成果(英) 35件
利用者成果(日) 0件

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Reference(英)
Puck, T. T. et al    J Exp Med  1958  108:945 
Kao, F T, Puck, T T  Genetics of somatic mammalian cells, VII. Induction and isolation of nutritional mutants in Chinese hamster cells.  Proc Natl Acad Sci U S A  1968  60:1275-81 

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Reference(日)
荒谷康昭    生体の科学  1992  43:444 

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利用者成果(英)
Mehnati, Parinaz, Morimoto, Shigeko, Yatagai, Fumio, Furusawa, Yoshiya, Kobayashi, Yasuhiko, Wada, Seiichi, Kanai, Tatsuaki, Hanaoka, Fumio, Sasaki, Hiroshi  Exploration of &quot;over kill effect&quot; of high-LET Ar- and Fe-ions by evaluating the fraction of non-hit cell and interphase death.  J Radiat Res  2005  46:343-50 
Hirukawa, K. Miyazawa, K. Maeda, H. Kameyama, Y. Goto, S. and Togari, A.  Osteogenesis accelerated by CHO-K1 cells transfected with the IGF-I gene  Dentistry in Japan  2004  40:31-34 
Tamura M, Natori K, Kobayashi M, Miyamura T, Takeda N  Inhibition of attachment of virions of Norwalk virus to mammalian cells by soluble histone molecules.  Arch Virol  2003  148:1659-70 
Sasaki H, Yatagai F, Kanai T, Furusawa Y, Hanaoka F, Zhu, W G, Mehnati P  Dependence of induction of interphase death of Chinese hamster ovary cells exposed to accelerated heavy ions on linear energy transfer.  Radiat Res  1997  148:449-54 
Niidome T, Takaji K, Urakawa M, Ohmori N, Wada A, Hirayama T, Aoyagi H  Chain length of cationic alpha-helical peptide sufficient for gene delivery into cells.  Bioconjug Chem  1999  10:773-80 
Uchino T, Tokunaga H, Ando M, Utsumi H  Quantitative determination of OH radical generation and its cytotoxicity induced by TiO(2)-UVA treatment.  Toxicol In Vitro  2002  16:629-35 
Yamauchi, Yoshio, Abe-Dohmae, Sumiko, Yokoyama, Shinji  Differential regulation of apolipoprotein A-I/ATP binding cassette transporter A1-mediated cholesterol and phospholipid release.  Biochim Biophys Acta  2002  1585:43110 
Wada, Seiichi, Kurahayashi, Hidemitsu, Kobayashi, Yasuhiko, Funayama, Tomoo, Yamamoto, Kazuo, Natsuhori, Masahiro, Ito, Nobuhiko  The relationship between cellular radiosensitivity and radiation-induced DNA damage measured by the comet assay.  J Vet Med Sci  2003  65:471-7 
Shimizu, Takeshi, Hamabata, Takashi, Yoshiki, Akemi, Hori, Takeya, Ito, Satoru, Takeda, Yoshifumi, Hayashi, Hideo  An association of 27- and 40-kDa molecules with glycolipids that bind A-B bacterial enterotoxins to cultured cells.  Biochim Biophys Acta  2003  1612:186-94 
Wada, Seiichi, Khoa, Tran Van, Kobayashi, Yasuhiko, Funayama, Tomoo, Yamamoto, Kazuo, Natsuhori, Masahiro, Ito, Nobuhiko  Detection of radiation-induced apoptosis using the comet assay.  J Vet Med Sci  2003  65:1161-6 
Wada, Wataru, Maeshima, Akito, Zhang, You-Qing, Hasegawa, Yoshihisa, Kuwano, Hiroyuki, Kojima, Itaru  Assessment of the function of the betaC-subunit of activin in cultured hepatocytes.  Am J Physiol Endocrinol Metab  2004  287:E247-54 
Ichida, Fumitaka, Nishimura, Riko, Hata, Kenji, Matsubara, Takuma, Ikeda, Fumiyo, Hisada, Kunihiro, Yatani, Hirofumi, Cao, Xu, Komori, Toshihisa, Yamaguchi, Akira, Yoneda, Toshiyuki  Reciprocal roles of MSX2 in regulation of osteoblast and adipocyte differentiation.  J Biol Chem  2004  279:34015-22 
Saito F, Hirayasu K, Satoh T, Wang CW, Lusingu J, Arimori T, Shida K, Palacpac NMQ, Itagaki S, Iwanaga S, Takashima E, Tsuboi T, Kohyama M, Suenaga T, Colonna M, Takagi J, Lavstsen T, Horii T, Arase H.  Immune evasion of Plasmodium falciparum by RIFIN via inhibitory receptors.  Nature  2017  552:101-105  PubMed ID: 29186116
Wakisaka N, Miyasaka N, Koide T, Masuda M, Hiraki-Kajiyama T, Yoshihara Y.  An Adenosine Receptor for Olfaction in Fish.  Curr. Biol.  2017  27:1437-1447.e4  PubMed ID: 28502661
Obayashi H, Kawabe Y, Makitsubo H, Watanabe R, Kameyama Y, Huang S, Takenouchi Y, Ito A, Kamihira M.  Accumulative gene integration into a pre-determined site using Cre/loxP.  J. Biosci. Bioeng.  2011  113:381-8  PubMed ID: 22136795
Cao Y, Kimura S, Itoi T, Honda K, Ohtake H, Omasa T.  Fluorescence in situ hybridization using bacterial artificial chromosome (BAC) clones for the analysis of chromosome rearrangement in Chinese hamster ovary cells.  Methods  2011  56:418-23  PubMed ID: 22100493
Takegawa Y, Araki K, Fujitani N, Furukawa J, Sugiyama H, Sakai H, Shinohara Y.  Simultaneous analysis of heparan sulfate, chondroitin/dermatan sulfates, and hyaluronan disaccharides by glycoblotting-assisted sample preparation followed by single-step zwitter-ionic-hydrophilic interaction chromatography.  Anal. Chem.  2011  83:9443-9  PubMed ID: 22044073
Akasaka Y, Sakai A, Takasu K, Tsukahara M, Hatta A, Suzuki H, Inoue H.  Suppressive effects of glycyrrhetinic acid derivatives on tachykinin receptor activation and hyperalgesia.  J. Pharmacol. Sci.  2011  117:180-8  PubMed ID: 22032861
Cao Y, Kimura S, Itoi T, Honda K, Ohtake H, Omasa T.  Construction of BAC-based physical map and analysis of chromosome rearrangement in Chinese hamster ovary cell lines.  Biotechnol. Bioeng.  2011  109:1357-67  PubMed ID: 22012683
Hattori K, Sugiura S, Kanamori T.  Scaffold fabrication in a perfusion culture microchamber array chip by O(2) plasma bonding of poly(dimethylsiloxane) protected by a physical mask.  Biomicrofluidics  2011  5:22204  PubMed ID: 21799711
Koike M, Yutoku Y, Koike A.  Accumulation of p21 proteins at DNA damage sites independent of p53 and core NHEJ factors following irradiation.  Biochem. Biophys. Res. Commun.  2011  412:39-43  PubMed ID: 21787748
Yasunaga M, Manabe S, Matsumura Y.  New concept of cytotoxic immunoconjugate therapy targeting cancer-induced fibrin clots.  Cancer Sci.  2011  102:1396-402  PubMed ID: 21481097
Yazaki Y, Oyane A, Sogo Y, Ito A, Yamazaki A, Tsurushima H.  Control of gene transfer on a DNA-fibronectin-apatite composite layer by the incorporation of carbonate and fluoride ions.  Biomaterials  2011  32:4896-902  PubMed ID: 21458066
Tjeldhorn L, Iversen N, Sandvig K, Bergan J, Sandset PM, Skretting G.  Functional characterization of the protein C A267T mutation: evidence for impaired secretion due to defective intracellular transport.  BMC Cell Biol.  2010  11:67  PubMed ID: 20815936
Matsuzaki K, Harada A, Takeiri A, Tanaka K, Mishima M.  Whole cell-ELISA to measure the gammaH2AX response of six aneugens and eight DNA-damaging chemicals.  Mutat. Res.  2010  700:71-9  PubMed ID: 20580854
Lee BH, Oyane A, Tsurushima H, Shimizu Y, Sasaki T, Koshizaki N.  A new approach for hydroxyapatite coating on polymeric materials using laser-induced precursor formation and subsequent aging.  ACS Appl Mater Interfaces  2010  1:1520-4  PubMed ID: 20355955
Shikano N, Ogura M, Sagara J, Nakajima S, Kobayashi M, Baba T, Yamaguchi N, Iwamura Y, Kubota N, Kawai K.  Stimulation of 125I-3-iodo-alpha-methyl-L-tyrosine uptake in Chinese hamster ovary (CHO-K1) cells by tyrosine esters.  Nucl. Med. Biol.  2010  37:189-96  PubMed ID: 20152718
Nakahara Y, Imanishi S, Mitsumasu K, Kanamori Y, Iwata K, Watanabe M, Kikawada T, Okuda T.  Cells from an anhydrobiotic chironomid survive almost complete desiccation.  Cryobiology  2009  60:138-46  PubMed ID: 19850023
Shikano N, Nakajima S, Kotani T, Ogura M, Sagara J, Iwamura Y, Yoshimoto M, Kubota N, Ishikawa N, Kawai K.  Transport of D-[1-14C]-amino acids into Chinese hamster ovary (CHO-K1) cells: implications for use of labeled d-amino acids as molecular imaging agents.  Nucl. Med. Biol.  2007  34:659-65  PubMed ID: 17707806
Kikawada T, Saito A, Kanamori Y, Nakahara Y, Iwata K, Tanaka D, Watanabe M, Okuda T.  Trehalose transporter 1, a facilitated and high-capacity trehalose transporter, allows exogenous trehalose uptake into cells.  Proc. Natl. Acad. Sci. U.S.A.  2007  104:11585-90  PubMed ID: 17606922
Omasa T, Kim K, Hiramatsu S, Katakura Y, Kishimoto M, Enosawa S, Ohtake H.  Construction and evaluation of drug-metabolizing cell line for bioartificial liver support system.  Biotechnol. Prog.  2005  21:161-7  PubMed ID: 15903254
Ismail T, Shafi S, Srinivas J, Sarkar D, Qurishi Y, Khazir J, Alam MS, Kumar HM.  Synthesis and tyrosinase inhibition activity of trans-stilbene derivatives.  Bioorg. Chem.  2016  64:97-102  PubMed ID: 26773755
Takagi Y, Yamazaki T, Masuda K, Nishii S, Kawakami B, Omasa T.  Identification of regulatory motifs in the CHO genome for stable monoclonal antibody production.  Cytotechnology  2017  69:451-460  PubMed ID: 27544513
Matsuya Y, Tsutsumi K, Sasaki K, Yoshii Y, Kimura T, Date H.  Modeling cell survival and change in amount of DNA during protracted irradiation.  J. Radiat. Res.  2017  58:302-312  PubMed ID: 27974510
Kinashi Y, Yokomizo N, Takahashi S.  DNA Double-strand Breaks Induced byFractionated Neutron Beam Irradiation for Boron Neutron Capture Therapy.  Anticancer Res.  2017  37:1681-1685  PubMed ID: 28373429

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