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Inducible transgene expression in CHO cells using an artificial transcriptional activator with estrogen-binding domain
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Michinaga S, Nagata A, Ogami R, Ogawa Y, Hishinuma S.
Histamine H1 Receptor-Mediated JNK Phosphorylation Is Regulated by Gq Protein-Dependent but Arrestin-Independent Pathways.
Int J Mol Sci
2024
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Shiomi A, Kaneko T, Nishikawa K, Tsuchida A, Isoshima T, Sato M, Toyooka K, Doi K, Nishikii H, Shintaku H.
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Nat Commun
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Design and validation of functionalized redox-responsive hydrogel beads for high-throughput screening of antibody-secreting mammalian cells.
J Biosci Bioeng
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2024
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Bilateral regulation of EGFR activity and local PI(4,5)P2 dynamics in mammalian cells observed with superresolution microscopy.
Elife
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Satoru Egawa, Kai Sakurai, Yoko Takeo, Kyota Yoshinaga, Masashi Takei, Shigeki Owada, Gota Yamaguchi, Shunya Yokomae, Hidekazu Mimura, Yutaka Yamagata, Makina Yabashi, Mari Shimura, Takashi Kimura
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Isolation and Genomic Characterization of a Heat-Labile Enterotoxin 1-Producing Escherichia fergusonii Strain from a Human
Microbiol Spectr
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Ying B, Kawabe Y, Zheng F, Amamoto Y, Kamihira M.
High-Level Production of scFv-Fc Antibody Using an Artificial Promoter System with Transcriptional Positive Feedback Loop of Transactivator in CHO Cells
Cells
2023
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Ali H, Kobayashi M, Morito K, Hasi RY, Aihara M, Hayashi J, Kawakami R, Tsuchiya K, Sango K, Tanaka T.
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Biochim Biophys Acta Mol Cell Biol Lipids
2023
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FEBS J
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Cibacron blue 3G-A is a novel inhibitor of Otopetrin 1 (OTOP1), a proton channel.
Biochem Biophys Res Commun
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Michinaga S, Nagata A, Ogami R, Ogawa Y, Hishinuma S.
Differential regulation of histamine H1 receptor-mediated ERK phosphorylation by Gq proteins and arrestins.
Biochem Pharmacol
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Mol Biol Cell
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Biochim Biophys Acta Mol Cell Biol Lipids
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Feline XRCC4 undergoes rapid Ku-dependent recruitment to DNA damage sites
FEBS Open Bio
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J Bone Miner Metab
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AQP9 transports lactate in tumor-associated macrophages to stimulate an M2-like polarization that promotes colon cancer progression.
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Genes Cells
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Pediatr Res
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Araki R, Yasubuchi A, Ikegaya M, Hojo C, Tachioka H, Kawai K, Omote M, Kita A, Yabe T.
Ferulic acid alleviates abnormal behaviors in isolation-reared mice via 5-HT1A receptor partial agonist activity.
Psychopharmacology (Berl)
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A point-mutation in the C-domain of CMP-sialic acid synthetase leads to lethality of medaka due to protein insolubility.
Sci Rep
2021
11(1):23211
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Baba K, Kuwada S, Nakao A, Li X, Okuda N, Nishida A, Mitsuda S, Fukuoka N, Kakeya H, Kataoka T.
Different localization of lysosomal-associated membrane protein 1 (LAMP1) in mammalian cultured cell lines.
Histochem Cell Biol
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Terumitsu-Tsujita M, Kitaura H, Miura I, Kiyama Y, Goto F, Muraki Y, Ominato S, Hara N, Simankova A, Bizen N, Kashiwagi K, Ito T, Toyoshima Y, Kakita A, Manabe T, Wakana S, Takebayashi H, Igarashi H.
Glial pathology in a novel spontaneous mutant mouse of the Eif2b5 gene: a vanishing white matter disease model
J Neurochem
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Kobayashi C, Tanaka A, Yasuda T, Hishinuma S.
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Biochem Pharmacol
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Sialic acid sulfation is induced by the antibiotic treatment in mammalian cells.
Biosci Biotechnol Biochem
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Nakazawa K, Shichino Y, Iwasaki S, Shiina N.
Implications of RNG140 (caprin2)-mediated translational regulation in eye lens differentiation.
J Biol Chem
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Hara-Chikuma M, Tanaka M, Verkman AS, Yasui M.
Inhibition of aquaporin-3 in macrophages by a monoclonal antibody as potential therapy for liver injury.
Nat Commun
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Anxa2- and Ctsd-knockout CHO cell lines to diminish the risk of contamination with host cell proteins.
Biotechnol. Prog.
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ABCA1 transporter reduces amphotericin B cytotoxicity in mammalian cells.
Cell. Mol. Life Sci.
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Hanako Tauchi, Chikahiro Imashiro, Taiki Kuribara, Genichiro Fujii, Yuta Kurashina, Kiichiro Totani, Kenjiro Takemura
Effective and Intact Cell Detachment from a Clinically Ubiquitous Culture Flask by Combining Ultrasonic Wave Exposure and Diluted Trypsin
Biotechnology and Bioprocess Engineering
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Yamamoto S, Iwamaru Y, Shimizu Y, Ueda Y, Sato M, Yamaguchi K, Nakanishi J.
Epidermal growth factor-nanoparticle conjugates change the activity from anti-apoptotic to pro-apoptotic at membrane rafts.
Acta Biomater
2019
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Yachi Y, Yoshii Y, Matsuya Y, Mori R, Oikawa J, Date H.
Track Structure Study for Energy Dependency of Electrons and X-rays on DNA Double-Strand Break Induction.
Sci Rep
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Nakade S, Mochida K, Kunii A, Nakamae K, Aida T, Tanaka K, Sakamoto N, Sakuma T, Yamamoto T.
Biased genome editing using the local accumulation of DSB repair molecules system.
Nat Commun
2018
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Matsuya Y, McMahon SJ, Tsutsumi K, Sasaki K, Okuyama G, Yoshii Y, Mori R, Oikawa J, Prise KM, Date H.
Investigation of dose-rate effects and cell-cycle distribution under protracted exposure to ionizing radiation for various dose-rates.
Sci Rep
2018
8(1):8287
PubMed ID: 29844494
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Yasui M, Hiroshima M, Kozuka J, Sako Y, Ueda M.
Automated single-molecule imaging in living cells.
Nat Commun
2018
9(1):3061
PubMed ID: 30076305
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Hishinuma S, Tamura Y, Kobayashi C, Akatsu C, Shoji M.
Differential Regulation of Thermodynamic Binding Forces of Levocetirizine and (S)-Cetirizine by Lys191 in Human Histamine H₁ Receptors.
Int J Mol Sci
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Tomoki Ohkubo, Haruyuki Kinoshita, View ORCID ProfileToshiro Maekawa, Katsuyuki Kunida, Hiroshi Kimura, Shinya Kuroda, Teruo Fujii
Development of a microfluidic cell culture and monitoring system for intracellular signaling studies
bioRxiv
2018
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Hishinuma S, Komazaki H, Tsukamoto H, Hatahara H, Fukui H, Shoji M.
Ca2+ -dependent down-regulation of human histamine H1 receptors in Chinese hamster ovary cells.
J. Neurochem.
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PubMed ID: 29063596
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Transient Acceleration of Epidermal Growth Factor Receptor Dynamics Produces Higher-Order Signaling Clusters.
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Aizawa Y, Sunada S, Hirakawa H, Fujimori A, Kato TA, Uesaka M.
Design and evaluation of a novel flavonoid-based radioprotective agent utilizing monoglucosyl rutin
J Radiat Res
2018
59(3):272-281
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Yoshida S, Fujiwara K, Nishihara H, Kato T, Yashiro T, Kato Y.
Retinoic acid signalling is a candidate regulator of the expression of pituitary-specific transcription factor Prop1 in the developing rodent pituitary
J Neuroendocrinol
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Saito F, Hirayasu K, Satoh T, Wang CW, Lusingu J, Arimori T, Shida K, Palacpac NMQ, Itagaki S, Iwanaga S, Takashima E, Tsuboi T, Kohyama M, Suenaga T, Colonna M, Takagi J, Lavstsen T, Horii T, Arase H.
Immune evasion of Plasmodium falciparum by RIFIN via inhibitory receptors.
Nature
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Wakisaka N, Miyasaka N, Koide T, Masuda M, Hiraki-Kajiyama T, Yoshihara Y.
An Adenosine Receptor for Olfaction in Fish.
Curr. Biol.
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27:1437-1447.e4
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Identification of regulatory motifs in the CHO genome for stable monoclonal antibody production.
Cytotechnology
2017
69:451-460
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Modeling cell survival and change in amount of DNA during protracted irradiation.
J. Radiat. Res.
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Kinashi Y, Yokomizo N, Takahashi S.
DNA Double-strand Breaks Induced byFractionated Neutron Beam Irradiation for Boron Neutron Capture Therapy.
Anticancer Res.
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Rapid one-pot fabrication of magnetic calcium phosphate nanoparticles immobilizing DNA and iron oxide nanocrystals using injection solutions for magnetofection and magnetic targeting
Materials Today Chemistry
2017
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Shibuya Y, Haga N, Asano R, Nakazawa H, Hattori T, Takeda D, Sugiyama A, Kurotani R, Kumagai I, Umetsu M, Makabe K.
Generation of camelid VHH bispecific constructs via in-cell intein-mediated protein trans-splicing
Protein Eng Des Sel
2017
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Shim I, Choi K, Hirano S.
Oxidative stress and cytotoxic effects of silver ion in mouse lung macrophages J774.1 cells
J Appl Toxicol
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Ohta A, Tsunoda Y, Tamura Y, Iino K, Nishimura N, Nishihara H, Takanashi H, Yoshida S, Kato T, Kato Y.
Construction and expression of vectors encoding biologically active rodent gonadotropins
J Reprod Dev
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63(6):605-609
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Hishinuma S, Kosaka K, Akatsu C, Uesawa Y, Fukui H, Shoji M.
Asp73-dependent and -independent regulation of the affinity of ligands for human histamine H 1 receptors by Na
Biochem Pharmacol
2017
128:46-54
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Shubhra QTH, Oyane A, Araki H, Nakamura M, Tsurushima H.
Calcium phosphate nanoparticles prepared from infusion fluids for stem cell transfection: process optimization and cytotoxicity analysis
Biomater Sci
2017
5(5):972-981
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Nakayama K, Ohashi R, Shinoda Y, Yamazaki M, Abe M, Fujikawa A, Shigenobu S, Futatsugi A, Noda M, Mikoshiba K, Furuichi T, Sakimura K, Shiina N.
RNG105/caprin1, an RNA granule protein for dendritic mRNA localization, is essential for long-term memory formation
Elife
2017
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Watanabe S, Moniaga CS, Nielsen S, Hara-Chikuma M.
Aquaporin-9 facilitates membrane transport of hydrogen peroxide in mammalian cells.
Biochem. Biophys. Res. Commun.
2016
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Teye K, Numata S, Ishii N, Krol RP, Tsuchisaka A, Hamada T, Koga H, Karashima T, Ohata C, Tsuruta D, Saya H, Haftek M, Hashimoto T.
Isolation of All CD44 Transcripts in Human Epidermis and Regulation of Their Expression by Various Agents
PLoS One
2016
11(8):e0160952
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Glucosylated cholesterol in mammalian cells and tissues: formation and degradation by multiple cellular β-glucosidases
J Lipid Res
2016
57(3):451-63
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Kawabe Y, Shimomura T, Huang S, Imanishi S, Ito A, Kamihira M.
Targeted transgene insertion into the CHO cell genome using Cre recombinase-incorporating integrase-defective retroviral vectors
Biotechnol Bioeng
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113(7):1600-10
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Shimura M, Shindou H, Szyrwiel L, Tokuoka SM, Hamano F, Matsuyama S, Okamoto M, Matsunaga A, Kita Y, Ishizaka Y, Yamauchi K, Kohmura Y, Lobinski R, Shimizu I, Shimizu T.
Imaging of intracellular fatty acids by scanning X-ray fluorescence microscopy
FASEB J
2016
30(12):4149-4158
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Controlled superficial assembly of DNA-amorphous calcium phosphate nanocomposite spheres for surface-mediated gene delivery
Colloids Surf B Biointerfaces
2016
141:519-527
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Kawaguchi K, Okamoto T, Morita M, Imanaka T.
Translocation of the ABC transporter ABCD4 from the endoplasmic reticulum to lysosomes requires the escort protein LMBD1
Sci Rep
2016
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Hishinuma S, Nozawa H, Akatsu C, Shoji M.
C-terminal of human histamine H 1 receptors regulates their agonist-induced clathrin-mediated internalization and G-protein signaling
J Neurochem
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139(4):552-565
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Kitakaze K, Mizutani Y, Sugiyama E, Tasaki C, Tsuji D, Maita N, Hirokawa T, Asanuma D, Kamiya M, Sato K, Setou M, Urano Y, Togawa T, Otaka A, Sakuraba H, Itoh K.
Protease-resistant modified human β-hexosaminidase B ameliorates symptoms in GM2 gangliosidosis model
J Clin Invest
2016
126(5):1691-703
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Ikeda M, Kato K, Yamaguchi M, Hamada H, Nakamura K, Sugimoto Y.
Cell Surface Antibody Retention Influences In Vivo Antitumor Activity Mediated by Antibody-dependent Cellular Cytotoxicity
Anticancer Res
2016
36(11):5937-5944
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Mitsuhiro Matsuda, Makito Koga, Knut Woltjen, Eisuke Nishida, Miki Ebisuya
Synthetic lateral inhibition governs cell-type bifurcation with robust ratios
Nat Commun
2015
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Sakuma T, Takenaga M, Kawabe Y, Nakamura T, Kamihira M, Yamamoto T.
Homologous Recombination-Independent Large Gene Cassette Knock-in in CHO Cells Using TALEN and MMEJ-Directed Donor Plasmids
Int J Mol Sci
2015
16(10):23849-66
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Matsuya Y, Tsutsumi K, Sasaki K, Date H.
Evaluation of the cell survival curve under radiation exposure based on the kinetics of lesions in relation to dose-delivery time
J Radiat Res
2015
56(1):90-9
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Ayako Oyane, Nao Matsuoka, Kenji Koga, Yoshiki Shimizu, Maki Nakamura, Kenji Kawaguchi, Naoto Koshizaki, Yu Sogo, Atsuo Ito, Hidero Unuma
Laser-assisted biomimetic process for surface functionalization of titanium metal
Ayako Oyane, Nao Matsuoka, Kenji Koga, Yoshiki Shimizu, Maki Nakamura, Kenji Kawaguchi, Naoto Koshizaki, Yu Sogo, Atsuo Ito, Hidero Unuma
2015
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Shigeto H, Ikeda T, Kuroda A, Funabashi H.
A BRET-based homogeneous insulin assay using interacting domains in the primary binding site of the insulin receptor
Anal Chem
2015
87(5):2764-70
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Konoki K, Suga Y, Fuwa H, Yotsu-Yamashita M, Sasaki M.
Evaluation of gambierol and its analogs for their inhibition of human Kv1.2 and cytotoxicity
Bioorg Med Chem Lett
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