Cell No. : Cell Name
RCB0282 : NFSa Y83
update : 2024/08/14
|
| Comment | A subline of NFSa which is a mouse cell line derived from fibrosarcoma. Transplantable. |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Ando, Koichi
|
| Year of deposit |
1989
|
| Animal |
_mouse
< Mammals
|
| Strain name |
C3H
|
| Tissue |
fibrosarcoma
|
| Classification |
cancer
|
| History |
transferred to in vitro by Yok
|
| Memo_1 |
Mycoplasma was eliminated with MC-210.
|
|
Lifespan |
infinite
|
| Morphology |
epithelial-like
|
| Cellosaurus(Expasy) |
CVCL_H735
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
| Culture medium |
|
MEM + 10% FBS
|
| Antibiotics |
|
Free
|
|
Passage method |
|
0.25% Trypsin
|
Culture information
|
Passage ratio |
|
1 : 8 split
|
| SC frequency |
|
Subculture : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| SSLP(mouse) |
|
OK
|
| Isozyme |
|
LD, NP, MPI
|
| Chromosome mode |
|
66-70(50) : /67(4),68(7),69(10),70(12),71(6),72(9)/
|
| Reference information |
Reference |
1
|
| User's Publication |
5
|
| User's Publication |
|
14340
Fujiwara T, Yakoub MA, Chandler A, Christ AB, Yang G, Ouerfelli O, Rajasekhar VK, Yoshida A, Kondo H, Hata T, Tazawa H, Dogan Y, Moore MAS, Fujiwara T, Ozaki T, Purdue E, Healey JH.
CSF-1/CSF-1R Signaling Inhibitor Pexidartinib (PLX3397) Reprograms Tumor-Associated Macrophages and Stimulates T-Cell Infiltration in the Sarcoma Microenvironment
Mol Cancer Ther
2021
PubMed ID: 34088832
DOI: 10.1158/1535-7163.MCT-20-0591
|
|
9095
N Meshii, G Takahashi, S Okunaga, M Hamada, S Iwai, A Takasu, Y Ogawa, Y Yura
Enhancement of Systemic Tumor Immunity for Squamous Cell Carcinoma Cells by an Oncolytic Herpes Simplex Virus
Cancer Gene Ther
2013
20 (9), 493-8
PubMed ID: 23887644
DOI: 10.1038/cgt.2013.45
|
|
5371
Fujita M, Hayashi I, Yamashina S, Itoman M, Majima M.
Blockade of angiotensin AT1a receptor signaling reduces tumor growth, angiogenesis, and metastasis.
Biochem Biophys Res Commun
2002
294(2):441-7
PubMed ID: 12051731
DOI: 10.1016/S0006-291X(02)00496-5
|
|
17923
Matsumoto K, Saga Y, Ikemura T, Sakakura T, Chiquet-Ehrismann R.
The distribution of tenascin-X is distinct and often reciprocal to that of tenascin-C
J Cell Biol
1994
125(2):483-93
PubMed ID: 7512972
DOI: 10.1083/jcb.125.2.483
|
|
17890
Saga Y, Tsukamoto T, Jing N, Kusakabe M, Sakakura T.
Murine tenascin: cDNA cloning, structure and temporal expression of isoforms
Gene
1991
104(2):177-85
PubMed ID: 1717349
DOI: 10.1016/0378-1119(91)90248-a
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