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RCB0256 : BALL-1  update : 2018/07/06
CommentTypical human B cell leukemia.
Comment from the depositor
Restriction (Category)a
Terms and conditionsBasically, there is no restriction regarding academic use.
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.
Basic information Depositor Maekawa, T. (Ito,F.)
Year of deposit 1988
Animal human < Mammals
Genus Homo
Species sapiens
Gender Male
Tissue blood
Case history acute lymphoblastoid leukemia (B cell leukemia)
Classification cancer
Memo_1 Mycoplasma was eliminated with MC-210.
Lifespan infinite
Morphology lymphocyte-like
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR (human) OK
Virus (HIV) (-)
Virus (HTLV-1) (-)
Isozyme LD, NP
Chromosome mode 46-48(50) : 46(4),47(22),48(24)
deposit info
lot info
Reference information Reference 0
User's Publication 6

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User's Publication
Hoshi O, Ushiki T.  Atomic force microscopy imaging of human metaphase chromosomes in liquid.  Methods Mol. Biol.  2011  736:109-15  PubMed ID: 21660724
Ikeda K, Mizutani T, Hoshi O, Ushiki T, Haga H, Kawabata K.  Intermediate structure between chromatin fibers and chromosome revealed by mechanical stretching and SPM measurement.  Biochem. Biophys. Res. Commun.  2010  400:181-6  PubMed ID: 20719238
Ushiki T, Shigeno M, Hoshi O.  Techniques for imaging human metaphase chromosomes in liquid conditions by atomic force microscopy.  Nanotechnology  2008  19:384022  PubMed ID: 21832581
Ushiki T, Hoshi O.  Atomic force microscopy for imaging human metaphase chromosomes.  Chromosome Res.  2008  16:383-96  PubMed ID: 18461479
Engelhardt P, Merilainen J, Zhao F, Uchiyama S, Fukui K, Lehto VP.  Whole-mount immunoelectron tomography of chromosomes and cells.  Methods Mol. Biol.  2007  369:387-405  PubMed ID: 17656761
Higashi T, Miyakawa S, Uchiyama S, Matsunaga S, Takata H, Fujimoto S, Noda M, Terauchi A, Shimizu T, Oda M, Azuma T, Fukui K.  Generation of monoclonal antibodies against chromosomal antigens that have a high sequence similarity between human and mouse.  J. Biotechnol.  2005  120:262-72  PubMed ID: 16171883

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