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RCB0247 : 10T1/2  update : 2018/07/06
CommentClone 8, Contact-inhibited semi-normal
特性(日) In vitroでの化学発ガン試験及び放射線発ガン試験に利用される。これは寺島(放射線医学総合研)が保存していたclone 8 で、継代数はまだ若い。
寄託者特性(英)
寄託者特性(日)
使用条件a
使用条件(英)Basically, there is no restriction regarding academic use.
使用条件(日)学術研究における利用につきましては原則として使用制限はありません。
提供申込書類 依頼書C-0001.pdf   同意書(非営利)C-0003.pdf   同意書(営利)C-0003p.pdf  
提供同意書は、使用機関及び使用内容に応じて二種類あります。該当する提供同意書をご使用ください(詳細)。特許等の取得及び商業利用等は別途ご相談(cellbank.brc@riken.jp)を承ります。
細胞基本情報 寄託者 Terasima, Toyozo
樹立者 Reznikoff, Catherine A.
寄託日 1988
別名 10T1/2(clone8)
動物種 mouse < Mammals
動物系統名 C3H
embryo
採取組織 embryo
細胞分類 normal
細胞寿命 infinite
細胞形態 fibroblast-like
細胞培養情報
寄託時情報
ロット情報
培地・試薬情報 培地・試薬一覧はこちらをご覧ください。
培養形態 Adherent cells
培地 BME (2mM L-Glutamine) + 10% FBS
抗生物質 Free
継代方法 0.25% trypsin
播種細胞数 5 x 10^(4) cells/60 mm dish
継代または培地交換頻度 Subculture : once/week, Medium Renewal : 2 times/week
培養最適温度 37 ℃
二酸化炭素濃度 5 %
凍結培地 Medium + 10% DMSO
凍結方法 Slow freezing
マイコプラズマ (-)
アイソザイム検査 LD, NP
染色体検査 65-81(50) : /75(1),76(1),77(3),78(5),79(12),80(25)/
画像情報
寄託時情報
ロット情報
文献情報 Reference(英) 5件
Reference(日) 3件
利用者成果(英) 59件
利用者成果(日) 4件

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Reference(英)
Taylor, S M, Jones, P A  Multiple new phenotypes induced in 10T1/2 and 3T3 cells treated with 5-azacytidine.  Cell  1979  17:771-9 
Paranjpe M, del Ande Eaton S, Boone, C W  Neoplasms produced from C3H/10T 1/2 cells attached to plastic plates; saturation density, anchorage dependence and serum requirement of in vitro lines correlated with growth aggressiveness in vivo.  J Cell Physiol  1978  96:63-71 
Weintraub H, Davis R, Tapscott S, Thayer M, Krause M, Benezra R, Blackwell, T K, Turner D, Rupp R, Hollenberg S, et al.  The myoD gene family: nodal point during specification of the muscle cell lineage.  Science  1991  251:761-6 
Reznikoff, C A, Brankow, D W, Heidelberger C  Establishment and characterization of a cloned line of C3H mouse embryo cells sensitive to postconfluence inhibition of division.  Cancer Res  1973  33:3231-8 
Terasima T, Yasukawa M, Kimura M  Radiation-induced transformation of 10T 1/2 mouse cells in the plateau phase: post-irradiation changes and serum dependence.  Gann  1981  72:762-8 

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Reference(日)
遠藤 剛  マウス:C3H10T1/2  生体の科学  1992  43:441 
達家雅明,角永武夫  バイオテクノロジ-素材としての培養細胞 増殖因子  蛋白質 核酸 酵素  1988  33:1230-1231 
黒木登志夫  バイオテクノロジ-素材としての培養細胞 化学物質によるトランスホ-メ-ションとプロモーション  蛋白質 核酸 酵素  1989  34:257-268 

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利用者成果(英)
Ouyang, Xinshou, Fujimoto, Minoru, Nakagawa, Reiko, Serada, Satoshi, Tanaka, Toshio, Nomura, Shintaro, Kawase, Ichiro, Kishimoto, Tadamitsu, Naka, Tetsuji  SOCS-2 interferes with myotube formation and potentiates osteoblast differentiation through upregulation of JunB in C2C12 cells.  J Cell Physiol  2006  207:428-36 
Funato N, Ohyama K, Kuroda T, Nakamura M  Basic helix-loop-helix transcription factor epicardin/capsulin/Pod-1 suppresses differentiation by negative regulation of transcription  J Biol Chem  2002  278:7486-7493 
Hata K, Nishimura R, Ikeda F, Yamashita K, Matsubara T, Nokubi T, Yoneda T  Differential roles of Smad1 and p38 kinase in regulation of peroxisome proliferator-activating receptor gamma during bone morphogenetic protein 2-induced adipogenesis.  Mol Biol Cell  2003  14:545-55 
Yano F, Kugimiya F, Ohba S, Ikeda T, Chikuda H, Ogasawara T, Ogata N, Takato T, Nakamura K, Kawaguchi H, Chung UI  The canonical Wnt signaling pathway promotes chondrocyte differentiation in a Sox9-dependent manner.  Biochem Biophys Res Commun  2005  333:1300-8 
Nakajima M, Negishi Y, Tanaka H, Kawashima K  p21(Cip-1/SDI-1/WAF-1) expression via the mitogen-activated protein kinase signaling pathway in insulin-induced chondrogenic differentiation of ATDC5 cells.  Biochem Biophys Res Commun  2004  320:1069-75 
Nobta M, Tsukazaki T, Shibata Y, Xin C, Moriishi T, Sakano S, Shindo H, Yamaguchi A  Critical regulation of bone morphogenetic protein-induced osteoblastic differentiation by Delta1/Jagged1-activated Notch1 signaling.  J Biol Chem  2005  280:15842-8 
Kawai S, Kato T, Inaba H, Okahashi N, Amano A  Odd-skipped related 2 splicing variants show opposite transcriptional activity.  Biochem Biophys Res Commun  2005  328:306-11 
Nakanishi K, Sudo T, Morishima N  Endoplasmic reticulum stress signaling transmitted by ATF6 mediates apoptosis during muscle development.  J Cell Biol  2005  169:555-60 
Katagiri T, Yamaguchi A, Ikeda T, Yoshiki S, Wozney, J M, Rosen V, Wang, E A, Tanaka H, Omura S, Suda T  The non-osteogenic mouse pluripotent cell line, C3H10T1/2, is induced to differentiate into osteoblastic cells by recombinant human bone morphogenetic protein-2.  Biochem Biophys Res Commun  1990  172:295-9 
Yamasaki K, Sasaki T, Nemoto M, Eto Y, Tajima N  Differentiation-induced insulin secretion from nonendocrine cells with engineered human proinsulin cDNA.  Biochem Biophys Res Commun  1999  265:361-5 
Maeda T, Abe M, Kurisu K, Jikko A, Furukawa S  Molecular cloning and characterization of a novel gene, CORS26, encoding a putative secretory protein and its possible involvement in skeletal development.  J Biol Chem  2001  276:3628-34 
Morishima, Nobuhiro, Nakanishi, Keiko, Takenouchi, Hiromi, Shibata, Takehiko, Yasuhiko, Yukuto  An endoplasmic reticulum stress-specific caspase cascade in apoptosis. Cytochrome c-independent activation of caspase-9 by caspase-12.  J Biol Chem  2002  277:34287-94 
Keller, Sascha, Nickel, Joachim, Zhang, Jin-Li, Sebald, Walter, Mueller, Thomas D  Molecular recognition of BMP-2 and BMP receptor IA.  Nat Struct Mol Biol  2004  11:481-8 
Ichida, Fumitaka, Nishimura, Riko, Hata, Kenji, Matsubara, Takuma, Ikeda, Fumiyo, Hisada, Kunihiro, Yatani, Hirofumi, Cao, Xu, Komori, Toshihisa, Yamaguchi, Akira, Yoneda, Toshiyuki  Reciprocal roles of MSX2 in regulation of osteoblast and adipocyte differentiation.  J Biol Chem  2004  279:34015-22 
Fujita, Takashi, Azuma, Yasutaka, Fukuyama, Ryo, Hattori, Yuji, Yoshida, Carolina, Koida, Masao, Ogita, Kiyokazu, Komori, Toshihisa  Runx2 induces osteoblast and chondrocyte differentiation and enhances their migration by coupling with PI3K-Akt signaling.  J Cell Biol  2004  166:85-95 
French, Dorothy M, Kaul, Raji J, D'Souza, Aloma L, Crowley, Craig W, Bao, Min, Frantz, Gretchen D, Filvaroff, Ellen H, Desnoyers, Luc  WISP-1 is an osteoblastic regulator expressed during skeletal development and fracture repair.  Am J Pathol  2004  165:855-67 
Morishima, Nobuhiro, Nakanishi, Keiko, Tsuchiya, Kyoko, Shibata, Takehiko, Seiwa, Emiko  Translocation of Bim to the endoplasmic reticulum (ER) mediates ER stress signaling for activation of caspase-12 during ER stress-induced apoptosis.  J Biol Chem  2004  279:50375-81 
Ishihara, Keiichi, Yamagishi, Nobuyuki, Hatayama, Takumi  Suppression of heat- and polyglutamine-induced cytotoxicity by nonsteroidal anti-inflammatory drugs.  Eur J Biochem  2004  271:4552-8 
Hata, Kenji, Nishimura, Riko, Ueda, Mio, Ikeda, Fumiyo, Matsubara, Takuma, Ichida, Fumitaka, Hisada, Kunihiro, Nokubi, Takashi, Yamaguchi, Akira, Yoneda, Toshiyuki  A CCAAT/enhancer binding protein beta isoform, liver-enriched inhibitory protein, regulates commitment of osteoblasts and adipocytes.  Mol Cell Biol  2005  25:1971-9 
Hinoi, Eiichi, Wang, Liyang, Takemori, Akihiro, Yoneda, Yukio  Functional expression of particular isoforms of excitatory amino acid transporters by rodent cartilage.  Biochem Pharmacol  2005  70:70-81 
Tamayama, Takumi, Maemura, Kentaro, Kanbara, Kiyoto, Hayasaki, Hana, Yabumoto, Yasuaki, Yuasa, Masayoshi, Watanabe, Masahito  Expression of GABA(A) and GABA(B) receptors in rat growth plate chondrocytes: activation of the GABA receptors promotes proliferation of mouse chondrogenic ATDC5 cells.  Mol Cell Biochem  2005  273:117-26 
Mellati A, Valizadeh Kiamahalleh M, Dai S, Bi J, Jin B, Zhang H.  Influence of polymer molecular weight on the in vitro cytotoxicity of poly (N-isopropylacrylamide).  Mater Sci Eng C Mater Biol Appl  2015  59:509-513  PubMed ID: 26652402
Sakai S, Liu Y, Sengoku M, Taya M.  Cell-selective encapsulation in hydrogel sheaths via biospecific identification and biochemical cross-linking.  Biomaterials  2015  53:494-501  PubMed ID: 25890746
Kawato Y, Hirao M, Ebina K, Tamai N, Shi K, Hashimoto J, Yoshikawa H, Myoui A.  Nkx3.2-induced suppression of Runx2 is a crucial mediator of hypoxia-dependent maintenance of chondrocyte phenotypes.  Biochem. Biophys. Res. Commun.  2011  416:205-10  PubMed ID: 22093831
Hatakeyama Y, Hatakeyama J, Takahashi A, Oka K, Tsuruga E, Inai T, Sawa Y.  The effect of valproic Acid on mesenchymal pluripotent cell proliferation and differentiation in extracellular matrices.  Drug Target Insights  2011  5:1-9  PubMed ID: 21904447
Yamagishi N, Yokota M, Yasuda K, Saito Y, Nagata K, Hatayama T.  Characterization of stress sensitivity and chaperone activity of Hsp105 in mammalian cells.  Biochem. Biophys. Res. Commun.  2011  409:90-5  PubMed ID: 21557931
Shimizu T, Tanaka T, Iso T, Matsui H, Ooyama Y, Kawai-Kowase K, Arai M, Kurabayashi M.  Notch signaling pathway enhances bone morphogenetic protein 2 (BMP2) responsiveness of Msx2 gene to induce osteogenic differentiation and mineralization of vascular smooth muscle cells.  J. Biol. Chem.  2011  286:19138-48  PubMed ID: 21471203
Mikami Y, Ishii Y, Watanabe N, Shirakawa T, Suzuki S, Irie S, Isokawa K, Honda MJ.  CD271/p75(NTR) inhibits the differentiation of mesenchymal stem cells into osteogenic, adipogenic, chondrogenic, and myogenic lineages.  Stem Cells Dev.  2010  20:901-13  PubMed ID: 21142793
Takarada-Iemata M, Takarada T, Nakamura Y, Nakatani E, Hori O, Yoneda Y.  Glutamate preferentially suppresses osteoblastogenesis than adipogenesis through the cystine/glutamate antiporter in mesenchymal stem cells.  J. Cell. Physiol.  2010  226:652-65  PubMed ID: 20717926
Kawai S, Abiko Y, Amano A.  Odd-skipped related 2 regulates genes related to proliferation and development.  Biochem. Biophys. Res. Commun.  2010  398:184-90  PubMed ID: 20599744
Kawai S, Amano A.  Odd-skipped related 2 is epigenetically regulated in cellular quiescence.  Biochem. Biophys. Res. Commun.  2010  396:831-6  PubMed ID: 20450884
Mikami Y, Asano M, Honda MJ, Takagi M.  Bone morphogenetic protein 2 and dexamethasone synergistically increase alkaline phosphatase levels through JAK/STAT signaling in C3H10T1/2 cells.  J. Cell. Physiol.  2009  223:123-33  PubMed ID: 20039267
Yuasa K, Masuda T, Yoshikawa C, Nagahama M, Matsuda Y, Tsuji A.  Subtilisin-like proprotein convertase PACE4 is required for skeletal muscle differentiation.  J. Biochem.  2009  146:407-15  PubMed ID: 19520771
Osawa K, Okubo Y, Nakao K, Koyama N, Bessho K.  Osteoinduction by microbubble-enhanced transcutaneous sonoporation of human bone morphogenetic protein-2.  J Gene Med  2009  11:633-41  PubMed ID: 19396932
Takahata Y, Takarada T, Iemata M, Yamamoto T, Nakamura Y, Kodama A, Yoneda Y.  Functional expression of beta2 adrenergic receptors responsible for protection against oxidative stress through promotion of glutathione synthesis after Nrf2 upregulation in undifferentiated mesenchymal C3H10T1/2 stem cells.  J. Cell. Physiol.  2008  218:268-75  PubMed ID: 18814142
Yamauchi M, Kawai S, Kato T, Ooshima T, Amano A.  Odd-skipped related 1 gene expression is regulated by Runx2 and Ikzf1 transcription factors.  Gene  2008  426:81-90  PubMed ID: 18804520
Kawai S, Yamauchi M, Wakisaka S, Ooshima T, Amano A.  Zinc-finger transcription factor odd-skipped related 2 is one of the regulators in osteoblast proliferation and bone formation.  J. Bone Miner. Res.  2007  22:1362-72  PubMed ID: 17547533
Hiroyama T, Miharada K, Aoki N, Fujioka T, Sudo K, Danjo I, Nagasawa T, Nakamura Y.  Long-lasting in vitro hematopoiesis derived from primate embryonic stem cells.  Exp. Hematol.  2006  34:760-9  PubMed ID: 16728281
Miharada K, Hiroyama T, Sudo K, Nagasawa T, Nakamura Y.  Lipocalin 2 functions as a negative regulator of red blood cell production in an autocrine fashion.  FASEB J.  2005  19:1881-3  PubMed ID: 16157692
Yano F, Kugimiya F, Ohba S, Ikeda T, Chikuda H, Ogasawara T, Ogata N, Takato T, Nakamura K, Kawaguchi H, Chung UI.  The canonical Wnt signaling pathway promotes chondrocyte differentiation in a Sox9-dependent manner.  Biochem. Biophys. Res. Commun.  2005  333:1300-8  PubMed ID: 15979579
Hata K, Nishimura R, Ueda M, Ikeda F, Matsubara T, Ichida F, Hisada K, Nokubi T, Yamaguchi A, Yoneda T.  A CCAAT/enhancer binding protein beta isoform, liver-enriched inhibitory protein, regulates commitment of osteoblasts and adipocytes.  Mol. Cell. Biol.  2005  25:1971-9  PubMed ID: 15713650
Nobta M, Tsukazaki T, Shibata Y, Xin C, Moriishi T, Sakano S, Shindo H, Yamaguchi A.  Critical regulation of bone morphogenetic protein-induced osteoblastic differentiation by Delta1/Jagged1-activated Notch1 signaling.  J. Biol. Chem.  2005  280:15842-8  PubMed ID: 15695512
Kawai S, Kato T, Inaba H, Okahashi N, Amano A.  Odd-skipped related 2 splicing variants show opposite transcriptional activity.  Biochem. Biophys. Res. Commun.  2005  328:306-11  PubMed ID: 15670784
Ishihara K, Yamagishi N, Hatayama T.  Suppression of heat- and polyglutamine-induced cytotoxicity by nonsteroidal anti-inflammatory drugs.  Eur. J. Biochem.  2004  271:4552-8  PubMed ID: 15560796
Fujita T, Azuma Y, Fukuyama R, Hattori Y, Yoshida C, Koida M, Ogita K, Komori T.  Runx2 induces osteoblast and chondrocyte differentiation and enhances their migration by coupling with PI3K-Akt signaling.  J. Cell Biol.  2004  166:85-95  PubMed ID: 15226309
Ichida F, Nishimura R, Hata K, Matsubara T, Ikeda F, Hisada K, Yatani H, Cao X, Komori T, Yamaguchi A, Yoneda T.  Reciprocal roles of MSX2 in regulation of osteoblast and adipocyte differentiation.  J. Biol. Chem.  2004  279:34015-22  PubMed ID: 15175325
Nishimura H, Nishimura M, Oda R, Yamanaka K, Matsubara T, Ozaki Y, Sekiya K, Hamada T, Kato Y.  Lectins induce resistance to proteases and/or mechanical stimulus in all examined cells--including bone marrow mesenchymal stem cells--on various scaffolds.  Exp. Cell Res.  2004  295:119-27  PubMed ID: 15051495
Hata K, Nishimura R, Ikeda F, Yamashita K, Matsubara T, Nokubi T, Yoneda T.  Differential roles of Smad1 and p38 kinase in regulation of peroxisome proliferator-activating receptor gamma during bone morphogenetic protein 2-induced adipogenesis.  Mol. Biol. Cell  2003  14:545-55  PubMed ID: 12589053
Funato N, Ohyama K, Kuroda T, Nakamura M.  Basic helix-loop-helix transcription factor epicardin/capsulin/Pod-1 suppresses differentiation by negative regulation of transcription.  J. Biol. Chem.  2002  278:7486-93  PubMed ID: 12493738
Mellati A, Kiamahalleh MV, Madani SH, Dai S, Bi J, Jin B, Zhang H.  Poly(N-isopropylacrylamide) hydrogel/chitosan scaffold hybrid for three-dimensional stem cell culture and cartilage tissue engineering.  J Biomed Mater Res A  2016  104:2764-74  PubMed ID: 27325374
Mellati A, Fan CM, Tamayol A, Annabi N, Dai S, Bi J, Jin B, Xian C, Khademhosseini A, Zhang H.  Microengineered 3D cell-laden thermoresponsive hydrogels for mimicking cell morphology and orientation in cartilage tissue engineering.  Biotechnol. Bioeng.  2017  114:217-231  PubMed ID: 27477393
Sakai S, Nomura K, Mochizuki K, Taya M.  Anchoring PEG-oleate to cell membranes stimulates reactive oxygen species production.  Colloids Surf B Biointerfaces  2016  147:336-342  PubMed ID: 27544656
Khanmohammadi M, Sakai S, Taya M.  Fabrication of single and bundled filament-like tissues using biodegradable hyaluronic acid-based hollow hydrogel fibers.  Int. J. Biol. Macromol.  2017  104:204-212  PubMed ID: 28596006
  Migration and Osteogenic Differentiation of Mesenchymal Stem Cells on Titanium with a MicroPatterned Gelatin Matrix        PubMed ID:
  Versatility of hydrogelation by dual-enzymatic reactions with oxidases and peroxidase        PubMed ID:
  Suppressive effect of mesenchymal stromal cells on interferon-γ-producing capability of spleen cells was specifically enhanced through humoral mediator(s) from mouse oral squamous cell carcinoma Sq-1979 Cells In Vitro        PubMed ID:
Hiroyama, Takashi, Miharada, Kenichi, Aoki, Naoko, Fujioka, Tsuyoshi, Sudo, Kazuhiro, Danjo, Inaho, Nagasawa, Toshiro, Nakamura, Yukio  Long-lasting in vitro hematopoiesis derived from primate embryonic stem cells.  Exp Hematol  2006  34:760-9 
Nishimura, Haruki, Nishimura, Masahiro, Oda, Ryo, Yamanaka, Katsuyuki, Matsubara, Takehiro, Ozaki, Yoshie, Sekiya, Kensuke, Hamada, Taizo, Kato, Yukio  Lectins induce resistance to proteases and/or mechanical stimulus in all examined cells--including bone marrow mesenchymal stem cells--on various scaffolds.  Exp Cell Res  2004  295:119-27 
Miharada, Ken-ichi, Hiroyama, Takashi, Sudo, Kazuhiro, Nagasawa, Toshiro, Nakamura, Yukio  Lipocalin 2 functions as a negative regulator of red blood cell production in an autocrine fashion.  FASEB J  2005  19:1881-3 

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利用者成果(日)
寛山隆、三原田賢一、青木尚子、須藤和寛、中村幸夫  10T1/2を用いた胚性幹細胞由来血液細胞の長期培養系の確立  第5回日本再生医療学会総会  2006   
石垣太郎、寛山隆、三原田賢一、藤岡剛、須藤和寛、檀上稲穂、長澤俊郎、中村幸夫  臍帯血培養系における Feeder細胞(OP9,10T1/2)の比較  第28回日本分子生物学会  2005   
寛山隆、三原田賢一、青木尚子、須藤和寛、中村幸夫  10T1/2を用いた胚性幹細胞由来血液細胞の長期培養系の確立  第5回日本再生医療学会総会  2006   
石垣太郎、寛山隆、三原田賢一、藤岡剛、須藤和寛、檀上稲穂、長澤俊郎、中村幸夫  臍帯血培養系における Feeder細胞(OP9,10T1/2)の比較  第28回日本分子生物学会  2005   



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