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RCB0247 : 10T1/2  update : 2018/07/06
CommentClone 8, Contact-inhibited semi-normal
Comment from the depositor
Terms and conditionsBasically, there is no restriction regarding academic use.
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.
Basic information Depositor Terasima, Toyozo
Originator Reznikoff, Catherine A.
Year of deposit 1988
Another name 10T1/2(clone8)
Animal mouse < Mammals
Strain name C3H
Age at sampling embryo
Tissue embryo
Classification normal
Lifespan infinite
Morphology fibroblast-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives BME (2mM L-Glutamine) + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage cell No 5 x 10^(4) cells/60 mm dish
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP (mouse) OK
Isozyme LD, NP
Chromosome mode 65-81(50) : /75(1),76(1),77(3),78(5),79(12),80(25)/
deposit info
lot info
Reference information Reference 5
User's Publication 65

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2000  Taylor, S M, Jones, P A  Multiple new phenotypes induced in 10T1/2 and 3T3 cells treated with 5-azacytidine.  Cell  1979  17:771-9  PubMed ID: 90553
44  Paranjpe M, del Ande Eaton S, Boone, C W  Neoplasms produced from C3H/10T 1/2 cells attached to plastic plates; saturation density, anchorage dependence and serum requirement of in vitro lines correlated with growth aggressiveness in vivo.  J Cell Physiol  1978  96:63-71  PubMed ID: 659520
2001  Weintraub H, Davis R, Tapscott S, Thayer M, Krause M, Benezra R, Blackwell, T K, Turner D, Rupp R, Hollenberg S, et al.  The myoD gene family: nodal point during specification of the muscle cell lineage.  Science  1991  251:761-6  PubMed ID: 1846704
961  Reznikoff, C A, Brankow, D W, Heidelberger C  Establishment and characterization of a cloned line of C3H mouse embryo cells sensitive to postconfluence inhibition of division.  Cancer Res  1973  33:3231-8  PubMed ID: 4357355
962  Terasima T, Yasukawa M, Kimura M  Radiation-induced transformation of 10T 1/2 mouse cells in the plateau phase: post-irradiation changes and serum dependence.  Gann  1981  72:762-8  PubMed ID: 7327374

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User's Publication
2644  Funato N, Ohyama K, Kuroda T, Nakamura M  Basic helix-loop-helix transcription factor epicardin/capsulin/Pod-1 suppresses differentiation by negative regulation of transcription  J Biol Chem  2002  278:7486-7493  PubMed ID: 12493738
2635  Hata K, Nishimura R, Ikeda F, Yamashita K, Matsubara T, Nokubi T, Yoneda T  Differential roles of Smad1 and p38 kinase in regulation of peroxisome proliferator-activating receptor gamma during bone morphogenetic protein 2-induced adipogenesis.  Mol Biol Cell  2003  14:545-55  PubMed ID: 12589053
2874  Yano F, Kugimiya F, Ohba S, Ikeda T, Chikuda H, Ogasawara T, Ogata N, Takato T, Nakamura K, Kawaguchi H, Chung UI  The canonical Wnt signaling pathway promotes chondrocyte differentiation in a Sox9-dependent manner.  Biochem Biophys Res Commun  2005  333:1300-8  PubMed ID: 15979579
2915  Nobta M, Tsukazaki T, Shibata Y, Xin C, Moriishi T, Sakano S, Shindo H, Yamaguchi A  Critical regulation of bone morphogenetic protein-induced osteoblastic differentiation by Delta1/Jagged1-activated Notch1 signaling.  J Biol Chem  2005  280:15842-8  PubMed ID: 15695512
2936  Kawai S, Kato T, Inaba H, Okahashi N, Amano A  Odd-skipped related 2 splicing variants show opposite transcriptional activity.  Biochem Biophys Res Commun  2005  328:306-11  PubMed ID: 15670784
1329  Katagiri T, Yamaguchi A, Ikeda T, Yoshiki S, Wozney, J M, Rosen V, Wang, E A, Tanaka H, Omura S, Suda T  The non-osteogenic mouse pluripotent cell line, C3H10T1/2, is induced to differentiate into osteoblastic cells by recombinant human bone morphogenetic protein-2.  Biochem Biophys Res Commun  1990  172:295-9  PubMed ID: 1699539
2378  Yamasaki K, Sasaki T, Nemoto M, Eto Y, Tajima N  Differentiation-induced insulin secretion from nonendocrine cells with engineered human proinsulin cDNA.  Biochem Biophys Res Commun  1999  265:361-5  PubMed ID: 10558872
2480  Maeda T, Abe M, Kurisu K, Jikko A, Furukawa S  Molecular cloning and characterization of a novel gene, CORS26, encoding a putative secretory protein and its possible involvement in skeletal development.  J Biol Chem  2001  276:3628-34  PubMed ID: 11071891
2893  Ichida, Fumitaka, Nishimura, Riko, Hata, Kenji, Matsubara, Takuma, Ikeda, Fumiyo, Hisada, Kunihiro, Yatani, Hirofumi, Cao, Xu, Komori, Toshihisa, Yamaguchi, Akira, Yoneda, Toshiyuki  Reciprocal roles of MSX2 in regulation of osteoblast and adipocyte differentiation.  J Biol Chem  2004  279:34015-22  PubMed ID: 15175325
2899  Fujita, Takashi, Azuma, Yasutaka, Fukuyama, Ryo, Hattori, Yuji, Yoshida, Carolina, Koida, Masao, Ogita, Kiyokazu, Komori, Toshihisa  Runx2 induces osteoblast and chondrocyte differentiation and enhances their migration by coupling with PI3K-Akt signaling.  J Cell Biol  2004  166:85-95  PubMed ID: 15226309
3343  Ishihara, Keiichi, Yamagishi, Nobuyuki, Hatayama, Takumi  Suppression of heat- and polyglutamine-induced cytotoxicity by nonsteroidal anti-inflammatory drugs.  Eur J Biochem  2004  271:4552-8  PubMed ID: 15560796
3183  Hata, Kenji, Nishimura, Riko, Ueda, Mio, Ikeda, Fumiyo, Matsubara, Takuma, Ichida, Fumitaka, Hisada, Kunihiro, Nokubi, Takashi, Yamaguchi, Akira, Yoneda, Toshiyuki  A CCAAT/enhancer binding protein beta isoform, liver-enriched inhibitory protein, regulates commitment of osteoblasts and adipocytes.  Mol Cell Biol  2005  25:1971-9  PubMed ID: 15713650
7113  Mellati A, Valizadeh Kiamahalleh M, Dai S, Bi J, Jin B, Zhang H.  Influence of polymer molecular weight on the in vitro cytotoxicity of poly (N-isopropylacrylamide).  Mater Sci Eng C Mater Biol Appl  2015  59:509-513  PubMed ID: 26652402
7378  Sakai S, Liu Y, Sengoku M, Taya M.  Cell-selective encapsulation in hydrogel sheaths via biospecific identification and biochemical cross-linking.  Biomaterials  2015  53:494-501  PubMed ID: 25890746
7632  Kawato Y, Hirao M, Ebina K, Tamai N, Shi K, Hashimoto J, Yoshikawa H, Myoui A.  Nkx3.2-induced suppression of Runx2 is a crucial mediator of hypoxia-dependent maintenance of chondrocyte phenotypes.  Biochem. Biophys. Res. Commun.  2011  416:205-10  PubMed ID: 22093831
7688  Hatakeyama Y, Hatakeyama J, Takahashi A, Oka K, Tsuruga E, Inai T, Sawa Y.  The effect of valproic Acid on mesenchymal pluripotent cell proliferation and differentiation in extracellular matrices.  Drug Target Insights  2011  5:1-9  PubMed ID: 21904447
7804  Yamagishi N, Yokota M, Yasuda K, Saito Y, Nagata K, Hatayama T.  Characterization of stress sensitivity and chaperone activity of Hsp105 in mammalian cells.  Biochem. Biophys. Res. Commun.  2011  409:90-5  PubMed ID: 21557931
7855  Shimizu T, Tanaka T, Iso T, Matsui H, Ooyama Y, Kawai-Kowase K, Arai M, Kurabayashi M.  Notch signaling pathway enhances bone morphogenetic protein 2 (BMP2) responsiveness of Msx2 gene to induce osteogenic differentiation and mineralization of vascular smooth muscle cells.  J. Biol. Chem.  2011  286:19138-48  PubMed ID: 21471203
8035  Mikami Y, Ishii Y, Watanabe N, Shirakawa T, Suzuki S, Irie S, Isokawa K, Honda MJ.  CD271/p75(NTR) inhibits the differentiation of mesenchymal stem cells into osteogenic, adipogenic, chondrogenic, and myogenic lineages.  Stem Cells Dev.  2010  20:901-13  PubMed ID: 21142793
8153  Takarada-Iemata M, Takarada T, Nakamura Y, Nakatani E, Hori O, Yoneda Y.  Glutamate preferentially suppresses osteoblastogenesis than adipogenesis through the cystine/glutamate antiporter in mesenchymal stem cells.  J. Cell. Physiol.  2010  226:652-65  PubMed ID: 20717926
8179  Kawai S, Abiko Y, Amano A.  Odd-skipped related 2 regulates genes related to proliferation and development.  Biochem. Biophys. Res. Commun.  2010  398:184-90  PubMed ID: 20599744
8272  Kawai S, Amano A.  Odd-skipped related 2 is epigenetically regulated in cellular quiescence.  Biochem. Biophys. Res. Commun.  2010  396:831-6  PubMed ID: 20450884
8407  Mikami Y, Asano M, Honda MJ, Takagi M.  Bone morphogenetic protein 2 and dexamethasone synergistically increase alkaline phosphatase levels through JAK/STAT signaling in C3H10T1/2 cells.  J. Cell. Physiol.  2009  223:123-33  PubMed ID: 20039267
8562  Yuasa K, Masuda T, Yoshikawa C, Nagahama M, Matsuda Y, Tsuji A.  Subtilisin-like proprotein convertase PACE4 is required for skeletal muscle differentiation.  J. Biochem.  2009  146:407-15  PubMed ID: 19520771
8595  Osawa K, Okubo Y, Nakao K, Koyama N, Bessho K.  Osteoinduction by microbubble-enhanced transcutaneous sonoporation of human bone morphogenetic protein-2.  J Gene Med  2009  11:633-41  PubMed ID: 19396932
8725  Takahata Y, Takarada T, Iemata M, Yamamoto T, Nakamura Y, Kodama A, Yoneda Y.  Functional expression of beta2 adrenergic receptors responsible for protection against oxidative stress through promotion of glutathione synthesis after Nrf2 upregulation in undifferentiated mesenchymal C3H10T1/2 stem cells.  J. Cell. Physiol.  2008  218:268-75  PubMed ID: 18814142
8727  Yamauchi M, Kawai S, Kato T, Ooshima T, Amano A.  Odd-skipped related 1 gene expression is regulated by Runx2 and Ikzf1 transcription factors.  Gene  2008  426:81-90  PubMed ID: 18804520
8803  Kawai S, Yamauchi M, Wakisaka S, Ooshima T, Amano A.  Zinc-finger transcription factor odd-skipped related 2 is one of the regulators in osteoblast proliferation and bone formation.  J. Bone Miner. Res.  2007  22:1362-72  PubMed ID: 17547533
9730  Mellati A, Kiamahalleh MV, Madani SH, Dai S, Bi J, Jin B, Zhang H.  Poly(N-isopropylacrylamide) hydrogel/chitosan scaffold hybrid for three-dimensional stem cell culture and cartilage tissue engineering.  J Biomed Mater Res A  2016  104:2764-74  PubMed ID: 27325374
9773  Mellati A, Fan CM, Tamayol A, Annabi N, Dai S, Bi J, Jin B, Xian C, Khademhosseini A, Zhang H.  Microengineered 3D cell-laden thermoresponsive hydrogels for mimicking cell morphology and orientation in cartilage tissue engineering.  Biotechnol. Bioeng.  2017  114:217-231  PubMed ID: 27477393
9786  Sakai S, Nomura K, Mochizuki K, Taya M.  Anchoring PEG-oleate to cell membranes stimulates reactive oxygen species production.  Colloids Surf B Biointerfaces  2016  147:336-342  PubMed ID: 27544656
10343  Khanmohammadi M, Sakai S, Taya M.  Fabrication of single and bundled filament-like tissues using biodegradable hyaluronic acid-based hollow hydrogel fibers.  Int. J. Biol. Macromol.  2017  104:204-212  PubMed ID: 28596006
11004    Migration and Osteogenic Differentiation of Mesenchymal Stem Cells on Titanium with a MicroPatterned Gelatin Matrix  International Journal of Theoretical and Applied Nanotechnology  2017  5:20-26 
11014    Versatility of hydrogelation by dual-enzymatic reactions with oxidases and peroxidase  Biochemical Engineering Journal  2018  131, 1-8 
11047    Suppressive effect of mesenchymal stromal cells on interferon-γ-producing capability of spleen cells was specifically enhanced through humoral mediator(s) from mouse oral squamous cell carcinoma Sq-1979 Cells In Vitro  Cancer Translational Medicine  2018  4, 9-16 
11132  Ito Y, Nakamura S, Sugimoto N, Shigemori T, Kato Y, Ohno M, Sakuma S, Ito K, Kumon H, Hirose H, Okamoto H, Nogawa M, Iwasaki M, Kihara S, Fujio K, Matsumoto T, Higashi N, Hashimoto K, Sawaguchi A, Harimoto KI, Nakagawa M, Yamamoto T, Handa M, Watanabe N, Nishi E, Arai F, Nishimura S, Eto K.  Turbulence Activates Platelet Biogenesis to Enable Clinical Scale Ex?Vivo Production.  Cell  2018    PubMed ID: 30017246
11182  Sakai S, Mochizuki K, Qu Y, Mail M, Nakahata M, Taya M.  Peroxidase-catalyzed microextrusion bioprinting of cell-laden hydrogel constructs in vaporized ppm-level hydrogen peroxide.  Biofabrication  2018    PubMed ID: 30137024
11138  Fukuda M, Yoshizawa T, Karim MF, Sobuz SU, Korogi W, Kobayasi D, Okanishi H, Tasaki M, Ono K, Sawa T, Sato Y, Chirifu M, Masuda T, Nakamura T, Tanoue H, Nakashima K, Kobashigawa Y, Morioka H, Bober E, Ohtsuki S, Yamagata Y, Ando Y, Oike Y, Araki N, Takeda S, Mizuta H, Yamagata K.  SIRT7 has a critical role in bone formation by regulating lysine acylation of SP7/Osterix.  Nat Commun  2018    PubMed ID: 30026585
11397  Gantumur E, Sakai S, Nakahata M, Taya M.  Horseradish peroxidase-catalyzed hydrogelation consuming enzyme-produced hydrogen peroxide in the presence of reducing sugars.  Soft Matter  2019    PubMed ID: 30672948
4059  Yoshida K, Teramachi J, Uchibe K, Ikegame M, Qiu L, Yang D, Okamura H.  Reduction of protein phosphatase 2A Cα promotes in vivo bone formation and adipocyte differentiation.  Mol. Cell. Endocrinol.  2018  470:251-258  PubMed ID: 29128580
4109  Kiyama R, Nonoyama T, Wada S, Semba S, Kitamura N, Nakajima T, Kurokawa T, Yasuda K, Tanaka S, Gong JP.  Micro patterning of hydroxyapatite by soft lithography on hydrogels for selective osteoconduction.  Acta Biomater  2018    PubMed ID: 30292679
4110  Takei H, Edahiro Y, Mano S, Masubuchi N, Mizukami Y, Imai M, Morishita S, Misawa K, Ochiai T, Tsuneda S, Endo H, Nakamura S, Eto K, Ohsaka A, Araki M, Komatsu N.  Skewed megakaryopoiesis in human induced pluripotent stem cell-derived haematopoietic progenitor cells harbouring calreticulin mutations.  Br. J. Haematol.  2018    PubMed ID: 29741776
3584  Muramatsu S, Wakabayashi M, Ohno T, Amano K, Ooishi R, Sugahara T, Shiojiri S, Tashiro K, Suzuki Y, Nishimura R, Kuhara S, Sugano S, Yoneda T, Matsuda A.  Functional gene screening system identified TRPV4 as a regulator of chondrogenic differentiation.  J Biol Chem  2007  282(44):32158-67  PubMed ID: 17804410
3926  Jimi E, Nakamura I, Amano H, Taguchi Y, Tsurukai T, Tamura M, Takahashi N, Suda T.  Osteoclast function is activated by osteoblastic cells through a mechanism involving cell-to-cell contact.  Endocrinology.  1996  137(8):2187-90  PubMed ID: 8754795
3808  Kinto N, Iwamoto M, Enomoto-Iwamoto M, Noji S, Ohuchi H, Yoshioka H, Kataoka H, Wada Y, Yuhao G, Takahashi HE, Yoshiki S, Yamaguchi A.  Fibroblasts expressing Sonic hedgehog induce osteoblast differentiation and ectopic bone formation.  FEBS Lett  1997  404(2-3):319-23  PubMed ID: 9119087
3937  Kobayashi T, Sugimoto T, Saijoh K, Fujii M, Chihara K.  Cloning and characterization of the 5'-flanking region of the mouse diastrophic dysplasia sulfate transporter gene.  Biochem Biophys Res Commun  1997  238(3):738-43  PubMed ID: 9325159
3921  Kobayashi T, Sugimoto T, Kanzawa M, Chihara K.  Identification of an enhancer sequence in 5'-flanking region of 1A exon of mouse liver/bone/kidney-type alkaline phosphatase gene.  Biochem Mol Biol Int  1998  44(4):683-91  PubMed ID: 9584982
3922  Yanaka N, Akatsuka H, Kawai E, Omori K.  1,25-Dihydroxyvitamin D3 upregulates natriuretic peptide receptor-C expression in mouse osteoblasts.  Am J Physiol  1998  275(6):E965-73  PubMed ID: 9843738
3968  Tamura M, Noda M.  Identification of DERMO-1 as a member of helix-loop-helix type transcription factors expressed in osteoblastic cells.  J Cell Biochem  1999  72(2):167-76  PubMed ID: 10022499
3969  Akiyama H1, Hiraki Y, Noda M, Shigeno C, Ito H, Nakamura T.  Molecular cloning and biological activity of a novel Ha-Ras suppressor gene predominantly expressed in skeletal muscle, heart, brain, and bone marrow by differential display using clonal mouse EC cells, ATDC5.  J Biol Chem  1999.  274(45):32192-7  PubMed ID: 10542256
3989  Yoshimasa FUJIMAKI  The Role of Recombinant Human Bone Morphogenetic Protein 2 (rhBMP-2) in the Differentiation of Osteoblasts and Chondrocytes  The Showa University Journal of Medical Sciences  2000  12:39-49 
3997  Enomoto-Iwamoto M, Nakamura T, Aikawa T, Higuchi Y, Yuasa T, Yamaguchi A, Nohno T, Noji S, Matsuya T, Kurisu K, Koyama E, Pacifici M, Iwamoto M.  Hedgehog proteins stimulate chondrogenic cell differentiation and cartilage formation.  J Bone Miner Res  2000  15(9):1659-68  PubMed ID: 10976986
3999  Hirata N et al  A chemical probe that labels human pluripotent stem cells.  Cell Rep  2014  6(6):1165-1174  PubMed ID: 24613351
5990  Kikuchi Y, Kume A, Urabe M, Mizukami H, Suzuki T, Ozaki K, Nagai T, Ozawa K.  Reciprocal upregulation of Notch signaling molecules in hematopoietic progenitor and mesenchymal stromal cells.  J Stem Cells Regen Med  2011  7(2):61-8  PubMed ID: 24693172
5991  Takayama N, Eto K.  In vitro generation of megakaryocytes and platelets from human embryonic stem cells and induced pluripotent stem cells.  Methods Mol Biol  2012  788:205-17  PubMed ID: 22130710
6001  Ogasawara T, Ohba S, Yano F, Kawaguchi H, Chung UI, Saito T, Yonehara Y, Nakatsuka T, Mori Y, Takato T, Hoshi K.  Nanog promotes osteogenic differentiation of the mouse mesenchymal cell line C3H10T1/2 by modulating bone morphogenetic protein (BMP) signaling.  J Cell Physiol  2013  228(1):163-71  PubMed ID: 22585661
6012  Zhao WH1, Hu ZQ.  Up-regulation of IL-33 expression in various types of murine cells by IL-3 and IL-4.  Cytokine  2012  58(2):267-73  PubMed ID: 22370606
6028  Kawamura F et al  Establishment of induced pluripotent stem cells from normal B cells and inducing AID expression in their differentiation into hematopoietic progenitor cells.  Sci Rep  2017  7(1):1659  PubMed ID: 28490810
6073  Nakagawa Y, Nakamura S, Nakajima M, Endo H, Dohda T, Takayama N, Nakauchi H, Arai F, Fukuda T, Eto K.  Two differential flows in a bioreactor promoted platelet generation from human pluripotent stem cell-derived megakaryocytes.  Exp Hematol  2013  41(8):742-8  PubMed ID: 23618622
6131  Ogaeri T, Eto K, Otsu M, Ema H, Nakauchi H.  The actin polymerization regulator WAVE2 is required for early bone marrow repopulation by hematopoietic stem cells.  Stem Cells  2009  27(5):1120-9  PubMed ID: 19415782
6157  Muramatsu M, Hanazono Y, Ogasawara Y, Okada T, Mizukami H, Kume A, Mizoguchi H, Ozawa K.  Reversible integration of the dominant negative retinoid receptor gene for ex vivo expansion of hematopoietic stem/progenitor cells.  Biochem Biophys Res Commun  2001  285(4):891-6  PubMed ID: 11467834
6232  Aoki H, Yamashita M, Hashita T, Nakayama M, Yagi M, Iwao T, Matsunaga T.  Isolation of induced pluripotent stem cell-derived endothelial progenitor cells from sac-like structures.  Biochem Biophys Res Commun  2019  515(4):672-678  PubMed ID: 31178142
3357  Hiroyama, Takashi, Miharada, Kenichi, Aoki, Naoko, Fujioka, Tsuyoshi, Sudo, Kazuhiro, Danjo, Inaho, Nagasawa, Toshiro, Nakamura, Yukio  Long-lasting in vitro hematopoiesis derived from primate embryonic stem cells.  Exp Hematol  2006  34:760-9  PubMed ID: 16728281
3158  Nishimura, Haruki, Nishimura, Masahiro, Oda, Ryo, Yamanaka, Katsuyuki, Matsubara, Takehiro, Ozaki, Yoshie, Sekiya, Kensuke, Hamada, Taizo, Kato, Yukio  Lectins induce resistance to proteases and/or mechanical stimulus in all examined cells--including bone marrow mesenchymal stem cells--on various scaffolds.  Exp Cell Res  2004  295:119-27  PubMed ID: 15051495
3159  Miharada, Ken-ichi, Hiroyama, Takashi, Sudo, Kazuhiro, Nagasawa, Toshiro, Nakamura, Yukio  Lipocalin 2 functions as a negative regulator of red blood cell production in an autocrine fashion.  FASEB J  2005  19:1881-3  PubMed ID: 16157692

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