Cell No. : Cell Name
RCB0243 : Nil2C2
update : 2023/06/30
|
| Comment | A fibroblastic cell line derived from a fetus of Syrian hamster. Transformed cells. Tumor formation and metastasis can be observed after transplantation. |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Sakiyama, Hisako
|
| Originator |
Diamond
|
| Year of deposit |
1988
|
| Animal |
_hamster
< Mammals
|
| Age at sampling |
embryo
|
| Tissue |
embryo
|
| Classification |
cancer
|
|
Lifespan |
infinite
|
| Morphology |
fibroblast-like
|
| Cellosaurus(Expasy) |
CVCL_8740
|
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| |
deposit info |
lot info |
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Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
| Culture medium |
|
MEM + 10% CS
|
| Antibiotics |
|
Free
|
|
Passage method |
|
0.25% Trypsin
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Culture information
|
Passage ratio |
|
1 : 8 split
|
| SC frequency |
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Subculture : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma |
|
(-)
|
| Animal PCR |
|
OK
|
| Isozyme |
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LD, NP
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| Reference information |
Reference |
11
|
| User's Publication |
0
|
| Reference |
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16
Nakagawa K, Sakiyama H, Fukazawa T, Matsumoto M, Takigawa M, Toyoguchi T, Moriya H.
Coordinated change between complement C1s production and chondrocyte differentiation in vitro
Cell Tissue Res
1997
289(2):299-305
PubMed ID: 9211832
DOI: 10.1007/s004410050876
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2779
Yamaguchi K, Kato N, Sakai N, Matsumoto M, Nagasawa S, Hatsuse H, Toyoguchi T, Moriya H, Sakiyama H
Immune complex independent activation of complement, C1s secreted from hamster embryo malignant fibroblasts, Nil2C2 in serum free culture medium.
Biochim Biophys Acta
1994
1205:133-8
PubMed ID: 8142477
DOI: 10.1016/0167-4838(94)90101-5
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2082
Sakiyama H, Yamaguchi K, Chiba K, Nagata K, Taniyama C, Matsumoto M, Suzuki G, Tanaka T, Tomoasawa T, Yasukawa M, et al.
Biochemical characterization and tissue distribution of hamster complement C1s.
J Immunol
1991
146:183-7
PubMed ID: 1898597
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2778
Sakiyama H, Nishino Y, Tanaka T, Tomosawa T, Kinoshita H, Nagata K, Chiba K, Sakiyama S
Purification and characterization of a novel calcium-dependent serine proteinase secreted from malignant hamster embryo fibroblast Nil2C2.
Biochim Biophys Acta
1989
990:156-61
PubMed ID: 2917176
DOI: 10.1016/s0304-4165(89)80028-5
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2081
Sakiyama H, Nishino Y, Nishimura K, Noda Y, Otsu H
Phagocytosis and solubilization of fixed cells by metastatic hamster embryo fibroblasts, Nil2C2.
Cancer Res
1984
44:2023-32
PubMed ID: 6370424
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2776
Sakiyama H, Terasima T
The synthesis of Forssman glycolipid in clones of nil 2 hamsters fibroblasts grown in monolayer or spinner culture.
Cancer Res
1975
35:1723-6
PubMed ID: 1169114
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2775
Sakiyama H, Robbins PW
Effect of transformation by hamster sarcoma virus on the glycolipid composition of secondary hamster embryo cells and the Nil cell line.
In Vitro
1974
9:331-8
PubMed ID: 4856904
DOI: 10.1007/BF02616016
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2774
Sakiyama H, Robbins PW
The effect of dibutyryl adenosine 3':5'-cyclic monophosphate on the synthesis of glycolipids by normal and transformed Nil cells.
Arch Biochem Biophys
1973
154:407-14
PubMed ID: 4347686
DOI: 10.1016/0003-9861(73)90073-8
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1075
Sakiyama H, Robbins, P W
Glycolipid synthesis and tumorigenicity of clones isolated from the Nil 2 line of hamster embryo fibroblasts.
Fed Proc
1973
32:86-90
PubMed ID: 4346327
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1074
Sakiyama H, Gross, S K, Robbins, P W
Glycolipid synthesis in normal and virus-transformed hamster cell lines.
Proc Natl Acad Sci U S A
1972
69:872-6
PubMed ID: 4337244
DOI: 10.1073/pnas.69.4.872
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2080
Diamond L
Two spontaneously transformed cell lines derived from the same hamster embryo culture.
Int J Cancer
1967
2:143-52
PubMed ID: 6039764
DOI: 10.1002/ijc.2910020209
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