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RCB0222 : NB1RGB  update : 2019/02/05
CommentNormal human skin fibroblast, RCB original
Comment from the depositor
Restriction (Category)a
Terms and conditionsBasically, there is no restriction regarding academic use.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Ohno,T. & Kojima
Originator Ohno, T. & Kurita, K.
Year of deposit 1987
Animal human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Male
Age at sampling 3 days
Tissue skin
Classification normal
Year of origin 1987
Lifespan finite
Morphology fibroblast-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives MEM ALPHA + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR (human) OK
Virus (HIV) (-)
Isozyme LD, NP
Chromosome mode 46(50)
Images
deposit info
lot info
Reference information Reference 0
User's Publication 62


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Reference

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User's Publication
3431  Sugihara, Eiji, Kanai, Masayuki, Saito, Soichiro, Nitta, Takayuki, Toyoshima, Hideo, Nakayama, Keiko, Nakayama, Keiichi I, Fukasawa, Kenji, Schwab, Manfred, Saya, Hideyuki, Miwa, Masanao  Suppression of centrosome amplification after DNA damage depends on p27 accumulation.  Cancer Res  2006  66:4020-9  PubMed ID: 16618721
1973  Kojima, H., Hanamura, A., Sato, A., and Konishi, H.  Comparison of Cytotoxicity of Surfactants on a Range of Mammalian Cells Cultured under Various Conditions  Alternatives to Animal Testing and Experimentation(AATEX)  1995  3:111-120 
2012  Kojima, H., Hanamura, A., Miyamoto, S., Sato, A., Konishi, H., and Yoshimura, I.  Evaluation of Seven Alternative Assays on the Main Ingredients in Cosmetics as Predictors of Draize Eye Irritation Scores  Toxicol in Vitro  1995  9:333-339 
2826  Takahagi Y, Fujimura T, Miyagawa S, Nagashima H, Shigehisa T, Shirakura R, Murakami H  Production of alpha 1,3-galactosyltransferase gene knockout pigs expressing both human decay-accelerating factor and N-acetylglucosaminyltransferase III.  Mol Reprod Dev  2005  71:331-8  PubMed ID: 15806557
2901  Takami Y, Motoki T, Yamamoto I, Gohda E  Synergistic induction of hepatocyte growth factor in human skin fibroblasts by the inflammatory cytokines interleukin-1 and interferon-gamma.  Biochem Biophys Res Commun  2005  327:212-7  PubMed ID: 15629451
1781  Obata K, Iwata K, Okada Y, Kohrin Y, Ohuchi E, Yoshida S, Shinmei M, Hayakawa T  A one-step sandwich enzyme immunoassay for human matrix metalloproteinase 3 (stromelysin-1) using monoclonal antibodies.  Clin Chim Acta  1992  211:59-72 
1808  Matsunaga T, Gohda E, Takebe T, Wu, Y L, Iwao M, Kataoka H, Yamamoto I  Expression of hepatocyte growth factor is up-regulated through activation of a cAMP-mediated pathway.  Exp Cell Res  1994  210:326-35  PubMed ID: 7507855
2277  Gohda E, Matsunaga T, Kataoka H, Takebe T, Yamamoto I  Induction of hepatocyte growth factor in human skin fibroblasts by epidermal growth factor, platelet-derived growth factor and fibroblast growth factor.  Cytokine  1994  6:633-40 
1970  Kondo H, Yonezawa Y  Fetal-adult phenotype transition, in terms of the serum dependency and growth factor requirements, of human skin fibroblast migration.  Exp Cell Res  1995  220:501-4 
1883  Fujimoto N, Hosokawa N, Iwata K, Shinya T, Okada Y, Hayakawa T  A one-step sandwich enzyme immunoassay for inactive precursor and complexed forms of human matrix metalloproteinase 9 (92 kDa gelatinase/type IV collagenase, gelatinase B) using monoclonal antibodies.  Clin Chim Acta  1994  231:79-88 
1841  Kobayashi S, Hirota Y, Sayato-Suzuki J, Takehana M, Nishimura H, Nishimura N, Tohyama C  Possible role of metallothionein in the cellular defense mechanism against UVB irradiation in neonatal human skin fibroblasts.  Photochem Photobiol  1994  59:650-6  PubMed ID: 8066125
1811  Fujimoto N, Mouri N, Iwata K, Ohuchi E, Okada Y, Hayakawa T  A one-step sandwich enzyme immunoassay for human matrix metalloproteinase 2 (72-kDa gelatinase/type IV collagenase) using monoclonal antibodies.  Clin Chim Acta  1993  221:91-103 
1810  Fujimoto N, Zhang J, Iwata K, Shinya T, Okada Y, Hayakawa T  A one-step sandwich enzyme immunoassay for tissue inhibitor of metalloproteinases-2 using monoclonal antibodies.  Clin Chim Acta  1993  220:31-45 
1782  Zhang J, Fujimoto N, Iwata K, Sakai T, Okada Y, Hayakawa T  A one-step sandwich enzyme immunoassay for human matrix metalloproteinase 1 (interstitial collagenase) using monoclonal antibodies.  Clin Chim Acta  1993  219:1-14 
2011  Matsuki H, Fujimoto N, Iwata K, Knauper V, Okada Y, Hayakawa T  A one-step sandwich enzyme immunoassay for human matrix metalloproteinase 8 (neutrophil collagenase) using monoclonal antibodies.  Clin Chim Acta  1996  244:129-43 
2358  Kimura M, Matsuda Y, Yoshioka T, Okano Y  Cell cycle-dependent expression and centrosome localization of a third human aurora/Ipl1-related protein kinase, AIK3.  J Biol Chem  1999  274:7334-40  PubMed ID: 10066797
2478  Suzuki M, Kase Y, Yamaguchi H, Kanai T, Ando K  Relative biological effectiveness for cell-killing effect on various human cell lines irradiated with heavy-ion medical accelerator in Chiba (HIMAC) carbon-ion beams.  Int J Radiat Oncol Biol Phys  2000  48:241-50  PubMed ID: 10924995
2479  Suzuki M, Kase Y, Kanai T, Ando K  Change in radiosensitivity with fractionated-dose irradiation of carbon-ion beams in five different human cell lines.  Int J Radiat Oncol Biol Phys  2000  48:251-8 
2357  Suzuki M, Kase Y, Kanai T, Ando K  Correlation between cell death and induction of non-rejoining PCC breaks by carbon-ion beams.  Adv Space Res  1998  22:561-8  PubMed ID: 11542786
2709  Yagi, Yasuyuki, Sotani, Tomohiro, Nagao, Toshinori, Horio, Tomoyo, Yamamoto, Itaru, Gohda, Eiichi  Induction by staurosporine of hepatocyte growth factor production in human skin fibroblasts independent of protein kinase inhibition.  Biochem Pharmacol  2003  66:1797-808  PubMed ID: 14563490
2937  Tsuruoka, Chizuru, Suzuki, Masao, Kanai, Tatsuaki, Fujitaka, Kazunobu  LET and ion species dependence for cell killing in normal human skin fibroblasts.  Radiat Res  2005  163:494-500  PubMed ID: 15850410
7135  Saito Y, Tsuruma K, Ichihara K, Shimazawa M, Hara H.  Brazilian green propolis water extract up-regulates the early expression level of HO-1 and accelerates Nrf2 after UVA irradiation.  BMC Complement Altern Med  2015  15:421  PubMed ID: 26611539
7296  Uto T, Tung NH, Appiah-Opong R, Aning A, Morinaga O, Edoh D, Nyarko AK, Shoyama Y.  Antiproliferative and Pro-Apoptotic Activity of Diarylheptanoids Isolated from the Bark of Alnus japonica in Human Leukemia Cell Lines.  Am. J. Chin. Med.  2015  43:757-67  PubMed ID: 26119959
7303  Kobayashi H, Nobeyama Y, Nakagawa H.  Tumor-suppressive effects of natural-type interferon-β through CXCL10 in melanoma.  Biochem. Biophys. Res. Commun.  2015  464:416-21  PubMed ID: 26102027
7536  K Shikano, K Chiba, S Miyata  Response of Human Skin Fibroblasts to Mechanical Stretch in Wound Healing Process Analyzed Using a Three-Dimensional Culture Model.  Advanced Biomedical Engineering  2015  4:170?178 
7544  Masahiro YO  Studies on Stem Cell Biology Using a Fluorescent Ubiquitination-based Cell Cycle Indicato.  Dissertation Submitted to the Graduate School of Life and Environmental Sciences, University of Tsukuba  2015   
7574  Yuji SHIBATA, Katsuhisa YAMADA, Chiaki IMADA, Takeshi KOBAYASHI, Takeshi TERAHARA  Phenotypic Characterization of a Microbe Producing Substances for Oxidative Stress Resistance Isolated from the Deep Seawater in Izu-Akazawa, Japan.  Deep Ocean Water Research  2015  15:117-124 
7780  Sprung CN, Cholewa M, Usami N, Kobayashi K, Crosbie JC.  DNA damage and repair kinetics after microbeam?radiation therapy emulation in living?cells using monoenergetic synchrotron X-ray microbeams.  J Synchrotron Radiat  2011  18:630-6  PubMed ID: 21685681
8158  Michikawa Y, Suga T, Ishikawa A, Hayashi H, Oka A, Inoko H, Iwakawa M, Imai T.  Genome wide screen identifies microsatellite markers associated with acute adverse effects following radiotherapy in cancer patients.  BMC Med. Genet.  2010  11:123  PubMed ID: 20701746
8280  Tsuruoka C, Furusawa Y, Anzai K, Okayasu R, Suzuki M.  Rejoining kinetics of G1-PCC breaks induced by different heavy-ion beams with a similar LET value.  Mutat. Res.  2010  701:47-51  PubMed ID: 20420934
8354  Takebayashi J, Ishii R, Chen J, Matsumoto T, Ishimi Y, Tai A.  Reassessment of antioxidant activity of arbutin: multifaceted evaluation using five antioxidant assay systems.  Free Radic. Res.  2010  44:473-8  PubMed ID: 20166881
8394  Bando M, Hiroshima Y, Kataoka M, Herzberg MC, Ross KF, Shinohara Y, Yamamoto T, Nagata T, Kido J.  Modulation of calprotectin in human keratinocytes by keratinocyte growth factor and interleukin-1alpha.  Immunol. Cell Biol.  2010  88:328-33  PubMed ID: 20065999
8515  Suzuki M, Tsuruoka C, Uchihori Y, Kitamura H, Liu CH.  Radiation-quality dependent cellular response in mutation induction in normal human cells.  J. Radiat. Res.  2009  50:395-9  PubMed ID: 19680011
8516  Tanaka H, Murakami Y, Ishii I, Nakata S.  Involvement of a forkhead transcription factor, FOXO1A, in UV-induced changes of collagen metabolism.  J. Investig. Dermatol. Symp. Proc.  2009  14:60-2  PubMed ID: 19675556
8673  Chizuru Tsuruoka, Masao Suzuki, Yoshiya Furusawa, Kazunori Anzai and Ryuichi Okayasu  The Influence of Mutation Induction in Normal Human Fibroblasts Irradiated with X Rays and Iron Ions  Biological Sciences in Space  2009  23:55-60 
8935  Horiba N, Itoh A, Yamaguchi M, Matsumoto T, Senda A, Nakamura H.  Effects of intracanal carbon dioxide laser irradiation on cultured human fibroblasts.  Oral Surg Oral Med Oral Pathol Oral Radiol Endod  2005  100:767-71  PubMed ID: 16301161
8957  Suzuki M, Tsuruoka C, Uchihori Y, Ebisawa S, Yasuda H, Fujitaka K.  Reduction in life span of normal human fibroblasts exposed to very low-dose-rate charged particles.  Radiat. Res.  2005  164:505-8  PubMed ID: 16187757
9133  Masao Suzuki, Chizuru Tsuruoka, Nakahiro Yasuda, Kenichi Matsumoto and Kazunobu Fujitaka  Bystander cellular effects in normal human fibroblasts irradiated with low-density carbon ions  International Congress Series  2005  1276:256-257 
9771  Li JR, Suzuki T, Nishimura H, Kishima M, Maeda S, Suzuki H.  Asymmetric Regulation of Peripheral Genes by Two Transcriptional Regulatory Networks.  PLoS ONE  2016  11:e0160459  PubMed ID: 27483142
9830  Yoshino A, Polouliakh N, Meguro A, Takeuchi M, Kawagoe T, Mizuki N.  Chum salmon egg extracts induce upregulation of collagen type I and exert antioxidative effects on human dermal fibroblast cultures.  Clin Interv Aging  2016  11:1159-68  PubMed ID: 27621603
9844  Horie M, Yamaguchi Y, Saito A, Nagase T, Lizio M, Itoh M, Kawaji H, Lassmann T, Carninci P, Forrest AR, Hayashizaki Y, Suzutani T, Kappert K, Micke P, Ohshima M.  Transcriptome analysis of periodontitis-associated fibroblasts by CAGE sequencing identified DLX5 and RUNX2 long variant as novel regulators involved in periodontitis.  Sci Rep  2016  6:33666  PubMed ID: 27645561
10021  Kanemaru A, Yamamoto K, Kawaguchi M, Fukushima T, Lin CY, Johnson MD, Camerer E, Kataoka H.  Deregulated matriptase activity in oral squamous cell carcinoma promotes the infiltration of cancer-associated fibroblasts by paracrine activation of protease-activated receptor 2.  Int. J. Cancer  2017  140:130-141  PubMed ID: 27615543
10059  Takashima S, Toyoshi K, Itoh T, Kajiwara N, Honda A, Ohba A, Takemoto S, Yoshida S, Shimozawa N.  Detection of unusual very-long-chain fatty acid and ether lipid derivatives in the fibroblasts and plasma of patients with peroxisomal diseases using liquid chromatography-mass spectrometry.  Mol. Genet. Metab.  2017  120:255-268  PubMed ID: 28089346
10431  Tamura I, Shirafuta Y, Jozaki K, Kajimura T, Shinagawa M, Maekawa R, Taketani T, Asada H, Sato S, Tamura H, Sugino N.  Novel Function of a Transcription Factor WT1 in Regulating Decidualization in Human Endometrial Stromal Cells and Its Molecular Mechanism.  Endocrinology  2017  158:3696-3707  PubMed ID: 28977591
10435  Tung NH, Uto T, Hai NT, Li G, Shoyama Y.  Quassinoids from the Root of Eurycoma longifolia and Their Antiproliferative Activity on Human Cancer Cell Lines.  Pharmacogn Mag  2017  13:459-462  PubMed ID: 28839372
10516  Bashar MK, Hashimoto K, Gotoh M, Kori H.  Analyzing orientation patterns of human skin fibroblasts using image gradient information.  Conf Proc IEEE Eng Med Biol Soc  2017  2017:600-603  PubMed ID: 29059944
10582  Nobeyama Y, Watanabe Y, Nakagawa H.  Silencing of G0/G1 switch gene 2 in cutaneous squamous cell carcinoma.  PLoS ONE  2017  12:e0187047  PubMed ID: 29073263
10673  Someya T, Sano K, Hara K, Sagane Y, Watanabe T, Wijesekara RGS.  Fibroblast and keratinocyte gene expression following exposure to extracts of neem plant (Azadirachta indica).  Data Brief  2018  16:982-992  PubMed ID: 29322079
10677  Yaekashiwa N, Otsuki S, Hayashi S, Kawase K.  Investigation of the non-thermal effects of exposing cells to 70-300 GHz irradiation using a widely tunable source.  J. Radiat. Res.  2018  59:116-121  PubMed ID: 29281029
10761  Uto T, Tung NH, Ohta T, Juengsanguanpornsuk W, Hung LQ, Hai NT, Long DD, Thuong PT, Okubo S, Hirata S, Shoyama Y.  Antiproliferative activity and apoptosis induction by trijuganone C isolated from the root of Salvia miltiorrhiza Bunge (Danshen).  Phytother Res  2018  32:657-666  PubMed ID: 29464799
10819  Mizuno M, Yaekashiwa N, Watanabe S.  Analysis of dermal composite conditions using collagen absorption characteristics in the THz range.  Biomed Opt Express  2018  9:2277-2283  PubMed ID: 29760986
10855  Junk  Engineering thick cell sheets by electrochemical desorption of oligopeptides on membrane substrates  Regenerative Therapy  2016  3 : 24-31 
10995    Distinction of dermal conditions related to collagen state using THz spectroscopy  IEEE Xplore:  2017   
2048  Suzuki, M., Kase, Y., Kanai, T., and Ando, K.  Correlation with Cell Death and Induction of Non-Rejoining PCC Breaks by Carbon Ion Beams    1996   
11263  Sano K, Kawanobe H, Someya T.  Effect of cupuassu butter on human skin cells.  Data Brief  2018    PubMed ID: 30370321
11266  Tezuka T, Ota A, Karnan S, Matsuura K, Yokoo K, Hosokawa Y, Vigetti D, Passi A, Hatano S, Umezawa K, Watanabe H.  The plant alkaloid conophylline inhibits matrix formation of fibroblasts.  J. Biol. Chem.  2018    PubMed ID: 30377255
11339  Yonezawa T, Momota R, Iwano H, Zhao S, Hakozaki T, Soh C, Sawaki S, Toyama K, Oohashi T.  Unripe peach (Prunus persica) extract ameliorates damage from UV irradiation and improved collagen XVIII expression in 3D skin model.  J Cosmet Dermatol  2018    PubMed ID: 30548159
11435  Iwasawa C, Tamura R, Sugiura Y, Suzuki S, Kuzumaki N, Narita M, Suematsu M, Nakamura M, Yoshida K, Toda M, Okano H, Miyoshi H.  Increased Cytotoxicity of Herpes Simplex Virus Thymidine Kinase Expression in Human Induced Pluripotent Stem Cells.  Int J Mol Sci  2019    PubMed ID: 30769780
4430  Shigeo Takashima, Kayoko Toyoshi, Nobuyuki Shimozawa  Analyses of the fatty acid separation principle using liquid chromatography-mass spectrometry  Medical Mass Spectrometry  2018  2 
4431    Verification of the non-thermal effects of THz-wave on human cells  IEEE Xplore  2018   
4306  Someya T, Sano K, Hara K, Sagane Y, Watanabe T, Wijesekara RGS.  Fibroblast and keratinocyte gene expression following exposure to the extracts of holy basil plant (Ocimum tenuiflorum), malabar nut plant (Justicia adhatoda), and emblic myrobalan plant (Phyllanthus emblica).  Data Brief  2018    PubMed ID: 29876372
4307  Sano K, Someya T, Hara K, Sagane Y, Watanabe T, Wijesekara RGS.  Effect of traditional plants in Sri Lanka on skin fibroblast cell number.  Data Brief  2018    PubMed ID: 29900360



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