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RCB0218 : MRC-5, known PDL  update : 2018/07/06
CommentNormal embryonic lung fibroblast with accurate PDL
Comment from the depositor
Terms and conditionsBasically, there is no restriction regarding academic use.
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.
Basic information Depositor Ohno, Tadao
Originator Jacobs, J. P.
Year of deposit 1987
Animal human < Mammals
Genus Homo
Species sapiens
Gender Male
Age at sampling 14 weeks embryo
Tissue embryo/fetus, lung
Classification normal
History Given directly from Dr. Jacobs
Lifespan finite
Crisis PDL 48< PDL
Morphology fibroblast-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives HF-RITC80-7 (or MEM ALPHA) + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR (human) OK
Virus (HIV) (-)
Isozyme LD, NP
Chromosome mode 42-47(50) : 42(3),43(3),44(6),45(12),46(24),47(2)
deposit info
lot info
Reference information Reference 0
User's Publication 7

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User's Publication
7848  Yasui T, Ohuchida K, Zhao M, Cui L, Onimaru M, Egami T, Fujita H, Ohtsuka T, Mizumoto K, Matsumoto K, Tanaka M.  Adenoviral therapy is more effective in gemcitabine-resistant pancreatic cancer than in gemcitabine-sensitive cells.  Anticancer Res.  2011  31:1279-87  PubMed ID: 21508376
8922  Nikawa H, Egusa H, Makihira S, Okamoto T, Kurihara H, Shiba H, Amano H, Murayama T, Yatani H, Hamada T.  An in vitro evaluation of the adhesion of Candida species to oral and lung tissue cells.  Mycoses  2005  49:14-7  PubMed ID: 16367812
10407  Hayakawa S, Shiratori S, Yamato H, Kameyama T, Kitatsuji C, Kashigi F, Goto S, Kameoka S, Fujikura D, Yamada T, Mizutani T, Kazumata M, Sato M, Tanaka J, Asaka M, Ohba Y, Miyazaki T, Imamura M, Takaoka A.  ZAPS is a potent stimulator of signaling mediated by the RNA helicase RIG-I during antiviral responses.  Nat. Immunol.  2011  12:37-44  PubMed ID: 21102435
10822  Takashina T, Koyama T, Nohara S, Hasegawa M, Ishiguro A, Iijima K, Lu J, Shimura M, Okamura T, Sakuma T, Yamamoto T, Ishizaka Y.  Identification of a cell-penetrating peptide applicable to a protein-based transcription activator-like effector expression system for cell engineering.  Biomaterials  2018  173:11-21  PubMed ID: 29734017
11194  Park AM, Tsunoda I, Yoshie O.  Heat shock protein 27 promotes cell cycle progression by down-regulating E2F transcription factor 4 and retinoblastoma family protein p130.  J. Biol. Chem.  2018    PubMed ID: 30166342
4305  Nakano T, Yamamura ET, Fujita H, Sone T, Asano K.  Novel methods for nucleotide length control in double-stranded polyinosinic-polycytidylic acid production using uneven length components.  Biosci. Biotechnol. Biochem.  2018    PubMed ID: 30079840
3786  Ogawa-Goto K, Irie S, Omori A, Miura Y, Katano H, Hasegawa H, Kurata T, Sata T, Arao Y.  An endoplasmic reticulum protein, p180, is highly expressed in human cytomegalovirus-permissive cells and interacts with the tegument protein encoded by UL48.  J Virol  2002  76(5):2350-62  PubMed ID: 11836413

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