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RCB0211 : MRC-5  update : 2018/07/06
CommentNormal embryonic lung fibroblast.
特性(日) ヒト胎児肺由来正常線維芽細胞。継代数は少ないが 正確な分裂齢は不明。ワクチン生産に多用されている。ECACCより入手した。
寄託者特性(英)
寄託者特性(日)
使用条件a
使用条件(英)Basically, there is no restriction regarding academic use.
使用条件(日)学術研究における利用につきましては原則として使用制限はありません。
提供申込書類 依頼書C-0001.pdf   同意書(非営利)C-0003.pdf   同意書(営利)C-0003p.pdf  
提供同意書は、使用機関及び使用内容に応じて二種類あります。該当する提供同意書をご使用ください(詳細)。特許等の取得及び商業利用等は別途ご相談(cellbank.brc@riken.jp)を承ります。
細胞基本情報 寄託者 ECACC
樹立者 Jacobs, J. P.
寄託日 1987
動物種 human < Mammals
属名 Homo
種名 sapiens
性別 Male
fetus
採取組織 lung
細胞分類 normal
入手歴 overseas, ECACC
細胞寿命 finite
細胞形態 fibroblast-like
細胞培養情報
寄託時情報
ロット情報
培地・試薬情報 培地・試薬一覧はこちらをご覧ください。
培養形態 Adherent cells
培地 MEM ALPHA (or HF-RITC80-7) + 10% FBS
抗生物質 Free
継代方法 0.25% trypsin
継代密度 1 : 4 split
継代または培地交換頻度 Subculture : 2 times/week
培養最適温度 37 ℃
凍結培地 Medium + 10% DMSO
凍結方法 Slow freezing
マイコプラズマ (-)
個体識別検査 OK
ウィルス(HIV) (-)
アイソザイム検査 LD, NP
染色体検査 44-47(50) : 44(3),45(7),46(39),47(1)
画像情報
寄託時情報
ロット情報
文献情報 Reference(英) 1件
Reference(日) 1件
利用者成果(英) 32件
利用者成果(日) 0件

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Reference(英)
Jacobs, J P, Jones, C M, Baille, J P  Characteristics of a human diploid cell designated MRC-5.  Nature  1970  227:168-70 

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Reference(日)
大野忠夫  バイオテクノロジ-素材としての培養細胞 ヒト2倍体細胞と老化  蛋白質 核酸 酵素  1989  34:1923-1924 

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利用者成果(英)
Okahira, Satoyo, Nishikawa, Fumiko, Nishikawa, Satoshi, Akazawa, Takashi, Seya, Tsukasa, Matsumoto, Misako  Interferon-beta induction through toll-like receptor 3 depends on double-stranded RNA structure.  DNA Cell Biol  2005  24:614-23 
Nikawa H, Egusa H, Makihira S, Okamoto T, Kurihara H, Shiba H, Amano H, Murayama T, Yatani H, Hamada T  An in vitro evaluation of the adhesion of Candida species to oral and lung tissue cells.  Mycoses  2006  49:14-7 
Yamashita, Satoko, Katayama, Hideki, Saitoh, Hiroyuki, Akao, Tetsuyuki, Park, Yu Shin, Mizuki, Eiichi, Ohba, Michio, Ito, Akio  Typical Three-Domain Cry Proteins of Bacillus thuringiensis Strain A1462 Exhibit Cytocidal Activity on Limited Human Cancer Cells.  J Biochem  2005  138:663-72 
Katayama H, Yokota H, Akao T, Nakamura O, Ohba M, Mekada E, Mizuki E  Parasporin-1, a novel cytotoxic protein to human cells from non-insecticidal parasporal inclusions of Bacillus thuringiensis  J Biochem (Tokyo)  2005  137:17-25 
Gohda E, Matsunaga T, Kataoka H, Yamamoto I  TGF-beta is a potent inhibitor of hepatocyte growth factor secretion by human fibroblasts.  Cell Biol Int Rep  1992  16:917-26 
Matsunaga T, Gohda E, Takebe T, Wu, Y L, Iwao M, Kataoka H, Yamamoto I  Expression of hepatocyte growth factor is up-regulated through activation of a cAMP-mediated pathway.  Exp Cell Res  1994  210:326-35 
Shindoh M, Higashino F, Kaya M, Yasuda M, Funaoka K, Hanzawa M, Hida K, Kohgo T, Amemiya A, Yoshida K, Fujinaga K  Correlated expression of matrix metalloproteinases and ets family transcription factor E1A-F in invasive oral squamous-cell-carcinoma-derived cell lines.  Am J Pathol  1996  148:693-700 
Saitoh T, Takemura S, Ueda K, Hosoya H, Nagayama M, Haga H, Kawabata K, Yamagishi A, Takahashi M  Differential localization of non-muscle myosin II isoforms and phosphorylated regulatory light chains in human MRC-5 fibroblasts.  FEBS Lett  2001  509:365-9 
Manabe, Tatsuya, Mizumoto, Kazuhiro, Nagai, Eishi, Matsumoto, Kunio, Nakamura, Toshikazu, Nukiwa, Toshihiro, Tanaka, Masao, Matsuda, Takehisa  Cell-based protein delivery system for the inhibition of the growth of pancreatic cancer: NK4 gene-transduced oral mucosal epithelial cell sheet.  Clin Cancer Res  2003  9:3158-66 
Yagi, Yasuyuki, Sotani, Tomohiro, Nagao, Toshinori, Horio, Tomoyo, Yamamoto, Itaru, Gohda, Eiichi  Induction by staurosporine of hepatocyte growth factor production in human skin fibroblasts independent of protein kinase inhibition.  Biochem Pharmacol  2003  66:1797-808 
Ito, Akio, Sasaguri, Yasuyuki, Kitada, Sakae, Kusaka, Yoshitomo, Kuwano, Kyoko, Masutomi, Kenjiro, Mizuki, Eiichi, Akao, Tetsuyuki, Ohba, Michio  A Bacillus thuringiensis crystal protein with selective cytocidal action to human cells.  J Biol Chem  2004  279:21282-6 
Ohuchida, Kenoki, Mizumoto, Kazuhiro, Ogura, Yasuhiro, Ishikawa, Nami, Nagai, Eishi, Yamaguchi, Koji, Tanaka, Masao  Quantitative assessment of telomerase activity and human telomerase reverse transcriptase messenger RNA levels in pancreatic juice samples for the diagnosis of pancreatic cancer.  Clin Cancer Res  2005  11:2285-92 
Yamamoto Y, Gotoh S, Korogi Y, Seki M, Konishi S, Ikeo S, Sone N, Nagasaki T, Matsumoto H, Muro S, Ito I, Hirai T, Kohno T, Suzuki Y, Mishima M.  Long-term expansion of alveolar stem cells derived from human iPS cells in organoids.  Nat. Methods  2017  14:1097-1106  PubMed ID: 28967890
Endo S, Nakata K, Ohuchida K, Takesue S, Nakayama H, Abe T, Koikawa K, Okumura T, Sada M, Horioka K, Zheng B, Mizuuchi Y, Iwamoto C, Murata M, Moriyama T, Miyasaka Y, Ohtsuka T, Mizumoto K, Oda Y, Hashizume M, Nakamura M.  Autophagy Is Required for Activation of Pancreatic Stellate Cells, Associated With Pancreatic Cancer Progression and Promotes Growth of Pancreatic Tumors in Mice.  Gastroenterology  2017  152:1492-1506.e24  PubMed ID: 28126348
Yasui T, Ohuchida K, Zhao M, Cui L, Onimaru M, Egami T, Fujita H, Ohtsuka T, Mizumoto K, Matsumoto K, Tanaka M.  Adenoviral therapy is more effective in gemcitabine-resistant pancreatic cancer than in gemcitabine-sensitive cells.  Anticancer Res.  2011  31:1279-87  PubMed ID: 21508376
Hirata N, Takahashi M, Yazawa M.  Diphosphorylation of regulatory light chain of myosin IIA is responsible for proper cell spreading.  Biochem. Biophys. Res. Commun.  2009  381:682-7  PubMed ID: 19254691
Yoshida K, Munakata H.  Connective tissue growth factor binds to fibronectin through the type I repeat modules and enhances the affinity of fibronectin to fibrin.  Biochim. Biophys. Acta  2007  1770:672-80  PubMed ID: 17239539
Yamashita S, Katayama H, Saitoh H, Akao T, Park YS, Mizuki E, Ohba M, Ito A.  Typical three-domain cry proteins of Bacillus thuringiensis strain A1462 exhibit cytocidal activity on limited human cancer cells.  J. Biochem.  2006  138:663-72  PubMed ID: 16428294
Nikawa H, Egusa H, Makihira S, Okamoto T, Kurihara H, Shiba H, Amano H, Murayama T, Yatani H, Hamada T.  An in vitro evaluation of the adhesion of Candida species to oral and lung tissue cells.  Mycoses  2005  49:14-7  PubMed ID: 16367812
Okahira S, Nishikawa F, Nishikawa S, Akazawa T, Seya T, Matsumoto M.  Interferon-beta induction through toll-like receptor 3 depends on double-stranded RNA structure.  DNA Cell Biol.  2005  24:614-23  PubMed ID: 16225392
Ohuchida K, Mizumoto K, Ogura Y, Ishikawa N, Nagai E, Yamaguchi K, Tanaka M.  Quantitative assessment of telomerase activity and human telomerase reverse transcriptase messenger RNA levels in pancreatic juice samples for the diagnosis of pancreatic cancer.  Clin. Cancer Res.  2005  11:2285-92  PubMed ID: 15788678
Katayama H, Yokota H, Akao T, Nakamura O, Ohba M, Mekada E, Mizuki E.  Parasporin-1, a novel cytotoxic protein to human cells from non-insecticidal parasporal inclusions of Bacillus thuringiensis.  J. Biochem.  2005  137:17-25  PubMed ID: 15713879
Ito A, Sasaguri Y, Kitada S, Kusaka Y, Kuwano K, Masutomi K, Mizuki E, Akao T, Ohba M.  A Bacillus thuringiensis crystal protein with selective cytocidal action to human cells.  J. Biol. Chem.  2004  279:21282-6  PubMed ID: 15026424
Yagi Y, Sotani T, Nagao T, Horio T, Yamamoto I, Gohda E.  Induction by staurosporine of hepatocyte growth factor production in human skin fibroblasts independent of protein kinase inhibition.  Biochem. Pharmacol.  2003  66:1797-808  PubMed ID: 14563490
Yagi Y, Sotani T, Nagao T, Horio T, Yamamoto I, Gohda E.  Induction by staurosporine of hepatocyte growth factor production in human skin fibroblasts independent of protein kinase inhibition.  Biochem. Pharmacol.  2003  66:1797-808  PubMed ID: 14563490
Manabe T, Mizumoto K, Nagai E, Matsumoto K, Nakamura T, Nukiwa T, Tanaka M, Matsuda T.  Cell-based protein delivery system for the inhibition of the growth of pancreatic cancer: NK4 gene-transduced oral mucosal epithelial cell sheet.  Clin. Cancer Res.  2003  9:3158-66  PubMed ID: 12912968
Yoshida K, Suzuki Y, Honda E, Amemiya K, Nakatani T, Ebina M, Narumi K, Satoh K, Munakata H.  Leucine-rich repeat region of decorin binds to filamin-A.  Biochimie  2002  84:303-8  PubMed ID: 12106908
Zhao FQ, Glimm DR, Kennelly JJ.  Distribution of mammalian facilitative glucose transporter messenger RNA in bovine tissues.  Int. J. Biochem.  1993  25:1897-903  PubMed ID: 8138027
Komiya K, Ohta S, Arima K, Ogawa M, Suzuki S, Mitamura Y, Nunomura S, Nanri Y, Yoshihara T, Kawaguchi A, Kadota JI, Rubin BK, Izuhara K.  Clarithromycin attenuates IL-13-induced periostin production in human lung fibroblasts.  Respir. Res.  2017  18:37  PubMed ID: 28219384
Hayakawa S, Shiratori S, Yamato H, Kameyama T, Kitatsuji C, Kashigi F, Goto S, Kameoka S, Fujikura D, Yamada T, Mizutani T, Kazumata M, Sato M, Tanaka J, Asaka M, Ohba Y, Miyazaki T, Imamura M, Takaoka A.  ZAPS is a potent stimulator of signaling mediated by the RNA helicase RIG-I during antiviral responses.  Nat. Immunol.  2011  12:37-44  PubMed ID: 21102435
Adachi Y, Watanabe K, Kita K, Kitai H, Kotani H, Sato Y, Inase N, Yano S, Ebi H.  Resistance mediated by alternative receptor tyrosine kinases in FGFR1-amplified lung cancer.  Carcinogenesis  2017  38:1063-1072  PubMed ID: 28968756
Takashina T, Koyama T, Nohara S, Hasegawa M, Ishiguro A, Iijima K, Lu J, Shimura M, Okamura T, Sakuma T, Yamamoto T, Ishizaka Y.  Identification of a cell-penetrating peptide applicable to a protein-based transcription activator-like effector expression system for cell engineering.  Biomaterials  2018  173:11-21  PubMed ID: 29734017

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