Cell No. : Cell Name RCB0211 : MRC-5  update : 2023/04/12
Comment			Distribution terminated. Normal embryonic lung fibroblast. Since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to the website of ECACC prior to order.
Comment from the depositor
Terms and conditions			There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	ECACC
		Originator	Jacobs, J. P.
		Year of deposit	1987
		Animal	_human < Mammals
		Genus	Homo
		Species	sapiens
		Gender	Male
		Age at sampling	fetus
		Tissue	lung
		Classification	normal
		History	overseas, ECACC
		Lifespan	finite
		Morphology	fibroblast-like
		Cellosaurus(Expasy)	CVCL_0440
			deposit info	lot info
		Medium	Medium List
		Culture type		Adherent cells
		Culture medium		MEM ALPHA (or HF-RITC80-7) + 10% FBS
		Antibiotics		Free
		Passage method		0.25% Trypsin
	Culture information	Passage ratio		1 : 4 split
		SC frequency		Subculture : 2 times/week
		Temperature		37 ℃
		CO2 concentration		5 %
		Freeze medium		Medium + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma		(-)
		Virus (HIV)		(-)
		Isozyme		LD, NP
		Chromosome mode		44-47(50) : 44(3),45(7),46(39),47(1)
		STR(human)		OK
Images			deposit info	lot info
Reference information		Reference	2
		User's Publication	7

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Reference

167  Jacobs JP.  The status of human diploid cell strain MRC-5 as an approved substrate for the production of viral vaccines  J Biol Stand  1976  4(2):97-9  PubMed ID: 932048   DOI: 10.1016/0092-1157(76)90018-4

168  Jacobs JP.  Updated results on the karyology of the WI-38, MRC-5 and MRC-9 cell strains  Dev Biol Stand  1976  37:155-6  PubMed ID: 1031681

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User's Publication

19992  Nishi M, Tateishi K, Sundararaj JS, Ino Y, Nakai Y, Hatayama Y, Yamaoka Y, Mihana Y, Miyakawa K, Kimura H, Kimura Y, Yamamoto T, Ryo A.  Development of a contacting transwell co-culture system for the in vitro propagation of primary central nervous system lymphoma  Front Cell Dev Biol  2023  Nov 27:11:1275519  PubMed ID: 38089883   DOI: 10.3389/fcell.2023.1275519

14787  Yamaoka Y, Miyakawa K, Jeremiah SS, Funabashi R, Okudela K, Kikuchi S, Katada J, Wada A, Takei T, Nishi M, Shimizu K, Ozawa H, Usuku S, Kawakami C, Tanaka N, Morita T, Hayashi H, Mitsui H, Suzuki K, Aizawa D, Yoshimura Y, Miyazaki T, Yamazaki E, Suzuki T, Kimura H, Shimizu H, Okabe N, Hasegawa H, Ryo A.  Highly specific monoclonal antibodies and epitope identification against SARS-CoV-2 nucleocapsid protein for antigen detection tests  Cell Rep Med  2021  2(6):100311  PubMed ID: 34027498   DOI: 10.1016/j.xcrm.2021.100311

4075  Kawasaki Y, Tsuboi C, Yagi K, Morizane M, Masaoka Y, Esumi S, Kitamura Y, Sendo T.  Photoinitiators enhanced 1,2-dichloropropane-induced cytotoxicity in human normal embryonic lung fibroblasts cells in vitro  Environ Sci Pollut Res Int  2015  22(6):4763-70  PubMed ID: 25501643   DOI: 10.1007/s11356-014-3939-8

5830  Hirai S, Endo S, Saito R, Hirose M, Ueno T, Suzuki H, Yamato K, Abei M, Hyodo I.  Antitumor effects of a sirtuin inhibitor, tenovin-6, against gastric cancer cells via death receptor 5 up-regulation.  PLoS One  2014  9(7):e102831  PubMed ID: 25033286   DOI: 10.1371/journal.pone.0102831

10293  Takunori Ueno, Shinji Endo, Rie Saito, Mitsuaki Hirose, Sachiko Hirai, Hideo Suzuki, Kenji Yamato, Ichinosuke Hyodo  The Sirtuin Inhibitor Tenovin-6 Upregulates Death Receptor 5 and Enhances Cytotoxic Effects of 5-Fluorouracil and Oxaliplatin in Colon Cancer Cells  Oncol Res  2013  21(3):155-64  PubMed ID: 24512730   DOI: 10.3727/096504013X13854886566598

15922  Fujitani N, Furukawa J, Araki K, Fujioka T, Takegawa Y, Piao J, Nishioka T, Tamura T, Nikaido T, Ito M, Nakamura Y, Shinohara Y.  Total cellular glycomics allows characterizing cells and streamlining the discovery process for cellular biomarkers  Proc Natl Acad Sci U S A   2013  110(6):2105-10  PubMed ID: 23345451   DOI: 10.1073/pnas.1214233110

10360  Masaaki K Sato, Masayuki Takahashi, Michio Yazawa  Two Regions of the Tail Are Necessary for the Isoform-Specific Functions of Nonmuscle Myosin IIB  Mol Biol Cell  2007  18(3):1009-17  PubMed ID: 17202408   DOI: 10.1091/mbc.e06-08-0706