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RCB0209 : SP2/0-Ag14  update : 2020/03/24
CommentMyeloma. Since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to the ECA 85072401 prior to order.
Comment from the depositor
Terms and conditionsBasically, there is no restriction regarding academic use. However, since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECA85072401 prior to order.
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.
Basic information Depositor ECACC
Originator Shulman, M. et al
Year of deposit 1987
Animal mouse < Mammals
Tissue myeloma
Case history myeloma
Classification cancer
History overseas, ECACC
Lifespan infinite
Morphology lymphocyte-like
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Chromosome mode 60-74(50) : /67(4),68(8),69(8),70(5),71(8),72(3)/
deposit info
lot info
Reference information Reference 2
User's Publication 10

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4662  Ozato K, Sachs DH.  Monoclonal antibodies to mouse MHC antigens. III. Hybridoma antibodies reacting to antigens of the H-2b haplotype reveal genetic control of isotype expression.  J Immunol  1981  126(1):317-21  PubMed ID: 6935293
335  Shulman M, Wilde, C D, Kohler G  A better cell line for making hybridomas secreting specific antibodies.  Nature  1978  276:269-70  PubMed ID: 714156

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User's Publication
10621  Akagi S, Nakajima C, Tanaka Y, Kurihara Y.  Flow cytometry-based method for rapid and high-throughput screening of hybridoma cells secreting monoclonal antibody.  J. Biosci. Bioeng.  2018  125:464-469  PubMed ID: 29174537
10753  Okuda T, Fukui A.  Generation of anti-oligosaccharide antibodies that recognize mammalian glycoproteins by immunization with a novel artificial glycosphingolipid.  Biochem. Biophys. Res. Commun.  2018  497:983-989  PubMed ID: 29458022
11070  Okuda T.  Data on immunoglobulin G antibodies induced by immunization of mice with globoside carrying very long-chain fatty acids  Data Brief  2018  19:256-260  PubMed ID: 29892643
4454  Hayashi Y, Okutani M, Ogawa S, Tsukahara T, Inoue R.  Generation of anti-porcine CD69 monoclonal antibodies and their usefulness to evaluate early activation of cellular immunity by flow cytometric analysis.  Anim. Sci. J.  2018  89:825-832  PubMed ID: 29460471
10249  Okuda T.  PUGNAc treatment provokes globotetraosylceramide accumulation in human umbilical vein endothelial cells.  Biochem. Biophys. Res. Commun.  2017  487:76-82  PubMed ID: 28392398
7490  Ohsawa H, Baba T, Enami J, Hiramatsu K.  Successful selection of an infection-protective anti-Staphylococcus aureus monoclonal antibody and its protective activity in murine infection models.  Microbiol. Immunol.  2015  59:183-92  PubMed ID: 25659598
9108  Magot Diata Omokoko, Sabar Pambudi, Supranee Phanthanawiboon, Promsin Masrinoul, Chayanee Setthapramote, Tadahiro Sasaki, Motoki Kuhara, Pongrama Ramasoota, Akifumi Yamashita, Itaru Hirai, Kazuyoshi Ikuta, Takeshi Kurosu  A Highly Conserved Region Between Amino Acids 221 and 266 of Dengue Virus Non-Structural Protein 1 Is a Major Epitope Region in Infected Patients  Am J Trop Med Hyg  2014  91 (1), 146-55  PubMed ID: 24778195
8541  Kubota-Koketsu R, Mizuta H, Oshita M, Ideno S, Yunoki M, Kuhara M, Yamamoto N, Okuno Y, Ikuta K.  Broad neutralizing human monoclonal antibodies against influenza virus from vaccinated healthy donors.  Biochem. Biophys. Res. Commun.  2009  387:180-5  PubMed ID: 19580789
8543  Kiyohara T, Totsuka A, Yoneyama T, Ishii K, Ito T, Wakita T.  Characterization of anti-idiotypic antibodies mimicking antibody- and receptor-binding sites on hepatitis A virus.  Arch. Virol.  2009  154:1263-9  PubMed ID: 19578927
5959  Komatsu Y, Tomizaki KY, Tsukamoto M, Kato T, Nishino N, Sato S, Yamori T, Tsuruo T, Furumai R, Yoshida M, Horinouchi S, Hayashi H.  Cyclic hydroxamic-acid-containing peptide 31, a potent synthetic histone deacetylase inhibitor with antitumor activity.  Cancer Res  2001  61(11):4459-66  PubMed ID: 11389076

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