Cell No. : Cell Name
RCB0208 : LTK-
update : 2023/06/30
|
| Comment | Thymidine kinase defective L cell. Since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECACC 85011432 prior to order. |
| Comment from the depositor | |
| Terms and conditions | 1) There is no restriction regarding use for basic researches. However, since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECA85011432 prior to order. 2) In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
ECACC
|
| Year of deposit |
1987
|
| Animal |
_mouse
< Mammals
|
| Tissue |
connective tissue
|
| Classification |
mutant
|
| History |
overseas
|
|
Lifespan |
infinite
|
| Morphology |
fibroblast-like
|
| Cellosaurus(Expasy) |
CVCL_3392
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
| Culture medium |
|
MEM + 10% CS
|
| Antibiotics |
|
Free
|
|
Passage method |
|
0.25% Trypsin
|
Culture information
|
Passage ratio |
|
1 : 8 split
|
| SC frequency |
|
Subculture : 1-2 times/week, Medium Renewal : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma |
|
(-)
|
| SSLP(mouse) |
|
OK
|
| Isozyme |
|
LD, NP
|
| Chromosome mode |
|
47-62(50) : 47(2),48(10),49(31),50(5),51(1),62(1)
|
| Reference information |
Reference |
3
|
| User's Publication |
6
|
| Reference |
|
169
Gupta RS, Dudani AK.
Species-specific differences in the toxicity of rhodamine 123 towards cultured mammalian cells
J Cell Physiol
1987
130(3):321-7
PubMed ID: 3558490
DOI: 10.1002/jcp.1041300303
|
|
955
Wigler M, Silverstein S, Lee, L S, Pellicer A, Cheng, Y c, Axel R
Transfer of purified herpes virus thymidine kinase gene to cultured mouse cells.
Cell
1977
11:223-32
PubMed ID: 194704
DOI: 10.1016/0092-8674(77)90333-6
|
|
166
HSU TC, MERCHANT DJ.
Mammalian chromosomes in vitro. XIV. Genotypic replacement in cell populations
J Natl Cancer Inst
1961
26:1075-83
PubMed ID: 13716385
|
| User's Publication |
|
7682
Bidet M, Joubert O, Lacombe B, Ciantar M, Nehme R, Mollat P, Bretillon L, Faure H, Bittman R, Ruat M, Mus-Veteau I.
The hedgehog receptor patched is involved in cholesterol transport.
PLoS ONE
2011
6:e23834
PubMed ID: 21931618
DOI: 10.1371/journal.pone.0023834
|
|
2375
Shimoyama Y, Takeda H, Yoshihara S, Kitajima M, Hirohashi S
Biochemical characterization and functional analysis of two type II classic cadherins, cadherin-6 and -14, and comparison with E-cadherin.
J Biol Chem
1999
274:11987-94
PubMed ID: 10207020
DOI: 10.1074/jbc.274.17.11987
|
|
3970
Kashima T, Kawaguchi J, Takeshita S, Kuroda M, Takanashi M, Horiuchi H, Imamura T, Ishikawa Y, Ishida T, Mori S, Machinami R, Kudo A.
Anomalous cadherin expression in osteosarcoma. Possible relationships to metastasis and morphogenesis.
Am J Pathol
1999
155(5):1549-55
PubMed ID: 10550312
DOI: 10.1016/S0002-9440(10)65471-5
|
|
5970
Yoshida K, Watanabe M, Kato H, Dutta A, Sugano S.
BH-protocadherin-c, a member of the cadherin superfamily, interacts with protein phosphatase 1 alpha through its intracellular domain.
FEBS Lett
1999
460(1):93-8
PubMed ID: 10571067
DOI: 10.1016/s0014-5793(99)01309-5
|
|
2374
Shimoyama Y, Shibata T, Kitajima M, Hirohashi S
Molecular cloning and characterization of a novel human classic cadherin homologous with mouse muscle cadherin.
J Biol Chem
1998
273:10011-8
PubMed ID: 9545347
DOI: 10.1074/jbc.273.16.10011
|
|
17225
Fukushige S, Ikeda JE.
Trapping of mammalian promoters by Cre-lox site-specific recombination
DNA Res
1996
3(2):73-80
PubMed ID: 8804858
DOI: 10.1093/dnares/3.2.73
|
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