Cell No. : Cell Name
RCB0207 : MRC-5 SV1 TG1
update : 2023/05/25
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| Comment | SV40-transformed, 6TG-resistant and HAT-sensitive. Since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECACC 85042501 prior to order. |
| Comment from the depositor | |
| Terms and conditions | 1) There is no restriction regarding use for basic researches. However, since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECA85042501 prior to order. 2) In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
ECACC
|
| Originator |
Jacobs, P.
|
| Year of deposit |
1987
|
| Original cell |
MRC-5
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Gender |
Male
|
| Age at sampling |
fetus
|
| Tissue |
lung
|
| Classification |
transformed
|
| History |
overseas, ECACC
|
|
Lifespan |
infinite
|
| Morphology |
fibroblast-like
|
| Cellosaurus(Expasy) |
CVCL_2625
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
| Culture medium |
|
HF-RITC80-7 (or MEM ALPHA) + 10% FBS
|
| Antibiotics |
|
Free
|
|
Passage method |
|
0.25% Trypsin
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Culture information
|
Passage ratio |
|
1 : 8 split
|
| SC frequency |
|
Subculture : once/week, Medium Renewal : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma |
|
(-)
|
| Virus (HIV) |
|
(-)
|
| Isozyme |
|
LD, NP
|
| Chromosome mode |
|
71-145(50) : /74(3),75(3),76(8),77(12),78(10),79(4)/
|
| STR(human) |
|
OK
|
| Reference information |
Reference |
2
|
| User's Publication |
18
|
| User's Publication |
|
17637
Kawashima D, Yuki T, Li S, Takei M.
Non-invasive imaging of ion concentration distribution around cell spheroids by electrical impedance tomographic sensor printed on circuit board under temporal compensation by ion transport impedance model
Biosens Bioelectron
2022
212:114432
PubMed ID: 35671699
DOI: 10.1016/j.bios.2022.114432
|
|
16512
Li S, Kawashima D, Sugawara M, Obara H, Okeyo KO, Takei M.
Study of transmembrane ion transport under tonicity imbalance using a combination of low frequency-electrical impedance spectroscopy (LF-EIS) and improved ion transport model
Biomed Phys Eng Express
2022
8(3)
PubMed ID: 35316798
DOI: 10.1088/2057-1976/ac5fc5
|
|
13256
Sakamoto Y, Kokuta T, Teshigahara A, Iijima K, Kitao H, Takata M, Tauchi H.
Mitotic cells can repair DNA double-strand breaks via a homology-directed pathway.
J Radiat Res
2021
PubMed ID: 33009557
DOI: 10.1093/jrr/rraa095
|
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14540
Sato N, Yao J, Sugawara M, Takei M.
Numerical Study of Particle-Fluid Flow Under AC Electrokinetics in Electrode-Multilayered Microfluidic Device
IEEE Trans Biomed Eng
2019
66(2):453-463
PubMed ID: 29993454
DOI: 10.1109/TBME.2018.2849004
|
|
10767
Kuragano M, Uyeda TQP, Kamijo K, Murakami Y, Takahashi M.
Different contributions of nonmuscle myosin IIA and IIB to the organization of stress fiber subtypes in fibroblasts.
Mol. Biol. Cell
2018
29:911-922
PubMed ID: 29467250
DOI: 10.1091/mbc.E17-04-0215
|
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10310
Sato Y, Murakami Y, Takahashi M.
Semi-retentive cytoskeletal fractionation (SERCYF): A novel method for the biochemical analysis of the organization of microtubule and actin cytoskeleton networks.
Biochem. Biophys. Res. Commun.
2017
488:614-620
PubMed ID: 28526408
DOI: 10.1016/j.bbrc.2017.05.083
|
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14443
Yao J, Sugawara M, Obara H, Mizutani T, Takei M.
Distinct Motion of GFP-Tagged Histone Expressing Cells Under AC Electrokinetics in Electrode-Multilayered Microfluidic Device
IEEE Trans Biomed Circuits Syst
2017
11(6):1450-1458
PubMed ID: 28809711
DOI: 10.1109/TBCAS.2017.2729584
|
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9543
Mizutani T, Haga H, Kawabata K.
Data set for comparison of cellular dynamics between human AAVS1 locus-modified and wild-type cells.
Data Brief
2016
6:793-8
PubMed ID: 26937449
DOI: 10.1016/j.dib.2015.12.053
|
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12924
Mizutani T, Furusawa K, Haga H, Kawabata K.
Heterogeneous filament network formation by myosin light chain isoforms effects on contractile energy output of single cardiomyocytes derived from human induced pluripotent stem cells
Regen Ther
2016
3:90-96
PubMed ID: 31245478
DOI: 10.1016/j.reth.2016.02.009
|
|
7253
Mizutani T, Li R, Haga H, Kawabata K.
Transgene integration into the human AAVS1 locus enhances myosin II-dependent contractile force by reducing expression of myosin binding subunit 85.
Biochem. Biophys. Res. Commun.
2015
465:270-4
PubMed ID: 26260320
DOI: 10.1016/j.bbrc.2015.08.018
|
|
14958
Ryosuke Yuchi, Jiafeng Yao, Takeomi Mizutani, Hiromichi Obara, Michiko Sugawara, Masahiro Takei
Analysis of dynamic cell movement under AC electric field in 3D multi-layered microchannel
2015 International Symposium on Micro-NanoMechatronics and Human Science (MHS)
2015
2015-11
DOI: 10.1109/mhs.2015.7438285
|
|
15954
Kiboku T, Katoh T, Nakamura A, Kitamura A, Kinjo M, Murakami Y, Takahashi M.
Nonmuscle myosin II folds into a 10S form via two portions of tail for dynamic subcellular localization
Genes Cells
2013
18(2):90-109
PubMed ID: 23237600
DOI: 10.1111/gtc.12021
|
|
10360
Masaaki K Sato, Masayuki Takahashi, Michio Yazawa
Two Regions of the Tail Are Necessary for the Isoform-Specific Functions of Nonmuscle Myosin IIB
Mol Biol Cell
2007
18(3):1009-17
PubMed ID: 17202408
DOI: 10.1091/mbc.e06-08-0706
|
|
3378
Kimura, Makoto T, Mori, Takahiro, Conroy, Jeffrey, Nowak, Norma J, Satomi, Susumu, Tamai, Katsuyuki, Nagase, Hiroki
Two functional coding single nucleotide polymorphisms in STK15 (Aurora-A) coordinately increase esophageal cancer risk.
Cancer Res
2005
65:3548-54
PubMed ID: 15867347
DOI: 10.1158/0008-5472.CAN-04-2149
|
|
2531
Masters JR, Thomson JA, Daly-Burns B, Reid YA, Dirks WG, Packer P, Toji LH, Ohno T, Tanabe H, Arlett CF, Kelland LR, Harrison M, Virmani A, Ward TH, Ayres KL, Debenham PG.
Short tandem repeat profiling provides an international reference standard for human cell lines.
Proc Natl Acad Sci U S A.
2001
98(14):8012-7
PubMed ID: 11416159
DOI: 10.1073/pnas.121616198
|
|
5548
Kim JK, Ryll R, Ishizuka Y, Kato S.
Identification of cDNAs encoding two novel nuclear proteins, IMUP-1 and IMUP-2, upregulated in SV40-immortalized human fibroblasts.
Gene
2000
257(2):327-34
PubMed ID: 11080599
DOI: 10.1016/s0378-1119(00)00414-5
|
|
9009
H Imamura, S Takao, T Aikou
A Modified invasion-3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium Bromide Assay for Quantitating Tumor Cell Invasion
Cancer Res
1994
54 (13), 3620-4
PubMed ID: 8012990
|
|
6243
Kodama S, Komatsu K, Okumura Y, Oshimura M.
Suppression of X-ray-induced chromosome aberrations in ataxia telangiectasia cells by introduction of a normal human chromosome 11.
Mutat Res
1992
293(1):31-7
PubMed ID: 1383808
DOI: 10.1016/0921-8777(92)90005-n
|
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