Cell No. : Cell Name RCB0197 : HUC-Fm  update : 2022/03/07
Comment			Normal umbilical cord fibroblast, male
Comment from the depositor
Terms and conditions			There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	Ohno,T. & Nishida,M.
		Originator	Ohno, Tadao
		Year of deposit	1987
		Animal	_human < Mammals
		Genus	Homo
		Species	sapiens
		Race	Japanese
		Gender	Male
		Tissue	umbilical cord
		Classification	normal
		Year of origin	1987
		Lifespan	finite
		Morphology	fibroblast-like
		Cellosaurus(Expasy)	CVCL_2524
			deposit info	lot info
		Medium	Medium List
		Culture type		Adherent cells
		Culture medium		HF-RITC80-7 + 10% FBS
		Antibiotics		Free
		Passage method		0.25% Trypsin
	Culture information	Passage ratio		1 : 4 split
		SC frequency		Subculture : 2 times/week
		Temperature		37 ℃
		CO2 concentration		5 %
		Freeze medium		Medium + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma		(-)
		Isozyme		LD, NP
		Chromosome mode		43-46(50) : 43(1),44(1),45(5),46(43)
		STR(human)		OK
Images			deposit info	lot info
Reference information		Reference	1
		User's Publication	5

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Reference

893  Sudo K, Kanno M, Miharada K, Ogawa S, Hiroyama T, Saijo K, Nakamura Y.  Mesenchymal progenitors able to differentiate into osteogenic, chondrogenic, and/or adipogenic cells in vitro are present in most primary fibroblast-like cell populations  Stem Cells  2007  25(7):1610-7  PubMed ID: 17395773   DOI: 10.1634/stemcells.2006-0504

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User's Publication

6028  Kawamura F　et al  Establishment of induced pluripotent stem cells from normal B cells and inducing AID expression in their differentiation into hematopoietic progenitor cells.  Sci Rep  2017  7(1):1659  PubMed ID: 28490810   DOI: 10.1038/s41598-017-01627-1

9109  Fujioka T, Shimizu N, Yoshino K, Miyoshi H, Nakamura Y.  Establishment of induced pluripotent stem cells from human neonatal tissues.  Hum Cell  2010  23(3):113-8  PubMed ID: 20973836   DOI: 10.1111/j.1749-0774.2010.00091.x

8788  Hiroyama T, Sudo K, Aoki N, Miharada K, Danjo I, Fujioka T, Nagasawa T, Nakamura Y.  Human umbilical cord-derived cells can often serve as feeder cells to maintain primate embryonic stem cells in a state capable of producing hematopoietic cells.  Cell Biol. Int.  2008  32:1-7  PubMed ID: 17890111   DOI: 10.1016/j.cellbi.2007.08.001

9428  Chirila T, Barnard Z, Zainuddin, Harkin DG, Schwab IR, Hirst L.  Bombyx mori silk fibroin membranes as potential substrata for epithelial constructs used in the management of ocular surface disorders.  Tissue Eng Part A  2008  14(7):1203-11  PubMed ID: 18380593   DOI: 10.1089/ten.tea.2007.0224

5545  Higuchi A, Yoshida M, Ohno T, Asakura T, Hara M.  Production of interferon-beta in a culture of fibroblast cells on some polymeric films.  Cytotechnology  2000  34(1-2):165-73  PubMed ID: 19003391   DOI: 10.1023/A:1008130223190