Back Back
Cell No. : Cell Name
RCB0169 : M1  update : 2018/07/06
CommentDifferentiate to macrophage and granulocyte
Comment from the depositor
Terms and conditionsBasically, there is no restriction regarding academic use.
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.
Basic information Depositor Ohno, Tadao
Originator Ichikawa, Yasuo
Year of deposit 1989
Animal mouse < Mammals
Tissue myeloid leukemia
Classification cancer
History Hozumi, Motoo.
Lifespan infinite
Morphology lymphocyte-like
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives (Earl's BSS with double conc. of AAs and Vit.) + 10% CS. Culturable in : MEM : DMEM (1 : 1) mixture + 10% CS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Others Require cloning for differntiation research.
deposit info
lot info
Reference information Reference 4
User's Publication 8

To topTop
5209  Maeda M, Ichikawa Y, Azuma I.  Differentiation and production of colony-stimulating factor induced by immunostimulants in a leukemia cell line.  J Cell Physiol  1980  105(1):33-8  PubMed ID: 6776132
5208  Maeda M, Horiuchi M, Numa S, Ichikawa Y.  Characterization of a differentiation-stimulating factor for mouse myeloid leukemia cells.  Gan  1977  68(4):435-47  PubMed ID: 308473
5207  Ichikawa Y, Maeda M, Horiuchi M.  Induction of differentiated functions which are reversibly suppressed by cytochalasin B.  Exp Cell Res  1975  90(1):20-30  PubMed ID: 123508
572  Ichikawa Y  Differentiation of a cell line of myeloid leukemia.  J Cell Physiol  1969  74:223-34  PubMed ID: 4187945

To topTop
User's Publication
8274  Kamdar RP, Matsumoto Y.  Radiation-induced XRCC4 association with chromatin DNA analyzed by biochemical fractionation.  J. Radiat. Res.  2010  51:303-13  PubMed ID: 20448413
3347  Nishio, Koji, Qiao, Shanlou, Yamashita, Hitoshi  Characterization of the differential expression of uncoupling protein 2 and ROS production in differentiated mouse macrophage-cells (Mm1) and the progenitor cells (M1).  J Mol Histol  2005  36:35-44  PubMed ID: 15703997
3180  Oikawa, Kensuke, Kimura, Shoji, Aoki, Naoko, Atsuta, Yoshiaki, Takiyama, Yumi, Nagato, Toshihiro, Yanai, Mitsuru, Kobayashi, Hiroya, Sato, Keisuke, Sasajima, Tadahiro, Tateno, Masatoshi  Neuronal calcium sensor protein visinin-like protein-3 interacts with microsomal cytochrome b5 in a Ca2+-dependent manner.  J Biol Chem  2004  279:15142-52  PubMed ID: 14739275
3114  Nochi, Hitoshi, Aoki, Naoko, Oikawa, Kensuke, Yanai, Mitsuru, Takiyama, Yumi, Atsuta, Yoshiaki, Kobayashi, Hiroya, Sato, Keisuke, Tateno, Masatoshi, Matsuno, Takeo, Katagiri, Makoto, Xing, Zhou, Kimura, Shoji  Modulation of hepatic granulomatous responses by transgene expression of DAP12 or TREM-1-Ig molecules.  Am J Pathol  2003  162:1191-201  PubMed ID: 12651611
3115  Aoki, Naoko, Kimura, Shoji, Oikawa, Kensuke, Nochi, Hitoshi, Atsuta, Yoshiaki, Kobayashi, Hiroya, Sato, Keisuke, Katagiri, Makoto  DAP12 ITAM motif regulates differentiation and apoptosis in M1 leukemia cells.  Biochem Biophys Res Commun  2002  291:296-304  PubMed ID: 11846404
3121  Aoki N, Kimura S, Takiyama Y, Atsuta Y, Abe A, Sato K, Katagiri M  The role of the DAP12 signal in mouse myeloid differentiation.  J Immunol  2000  165:3790-6  PubMed ID: 11034384
2376  Hosokawa, M., Minami, K., Kohno, H., Tanaka, Y., and Hibino, H.  Differentiation- and Apoptosis-inducing Activities of Phospolipids Containing Docosahexaenoic Acid for Mouse Myeloid Leukemia M1 Cells  Fisheries Science  1999  65:798-799 
1948  Tomita Mitsuko  Estimation and Analysis of Relative Amounts of Murine Attachmin Expressed in Cell Membranes of a Monocyte/Macrophage Lineage  Bioiamges  1995  3:107-112 

Back Back Return Top Page