Cell No. : Cell Name
RCB0162 : NHSF46
update : 2023/06/20
|
| Comment | Diploid fibroblast from normal Japanese skin. |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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|
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Basic information
|
Depositor |
Ohno, Tadao
|
| Originator |
Fujiwara, Yoshisada
|
| Year of deposit |
1986
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Race |
Japanese
|
| Gender |
Unknown
|
| Tissue |
skin
|
| Classification |
normal
|
|
Lifespan |
finite
|
| Crisis PDL |
45
PDL
|
| Morphology |
fibroblast-like
|
| Cellosaurus(Expasy) |
CVCL_G283
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
| Culture medium |
|
MEM ALPHA + 10% FBS
|
| Antibiotics |
|
Free
|
|
Passage method |
|
0.25% Trypsin
|
Culture information
|
Passage ratio |
|
1 : 4 split
|
| SC frequency |
|
Subculture : once/week, Medium Renewal : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma |
|
(-)
|
| Animal PCR |
|
OK
|
| Virus (HIV) |
|
(-)
|
| Isozyme |
|
LD, NP
|
| Chromosome mode |
|
41-91(50) : 41(1),43(1),44(1),45(3),46(41),47(2)/
|
| STR(human) |
|
OK
|
| Images |
deposit info | lot info |
|
|
|
| Reference information |
Reference |
0
|
| User's Publication |
7
|
| User's Publication |
|
5631
Uto T, Sakamoto A, Tung NH, Fujiki T, Kishihara K, Oiso S, Kariyazono H, Morinaga O, Shoyama Y.
Anti-Proliferative Activities and Apoptosis Induction by Triterpenes Derived from Eriobotrya japonica in Human Leukemia Cell Lines.
Int J Mol Sci
2013
14(2):4106-20
PubMed ID: 23429195
DOI: 10.3390/ijms14024106
|
|
7933
Yoshimitsu M, Higuchi K, Miyata M, Devine S, Mattman A, Sirrs S, Medin JA, Tei C, Takenaka T.
Identification of novel mutations in the α-galactosidase A gene in patients with Fabry disease: pitfalls of mutation analyses in patients with low α-galactosidase A activity.
J Cardiol
2011
57:345-53
PubMed ID: 21333496
DOI: 10.1016/j.jjcc.2010.12.004
|
|
7956
Uchino T, Ikarashi Y, Nishimura T.
Effects of coating materials and size of titanium dioxide particles on their cytotoxicity and penetration into the cellular membrane.
J Toxicol Sci
2011
36:95-100
PubMed ID: 21297346
DOI: 10.2131/jts.36.95
|
|
8330
Maeda H, Nakano T, Tomokiyo A, Fujii S, Wada N, Monnouchi S, Hori K, Akamine A.
Mineral trioxide aggregate induces bone morphogenetic protein-2 expression and calcification in human periodontal ligament cells.
J Endod
2010
36:647-52
PubMed ID: 20307738
DOI: 10.1016/j.joen.2009.12.024
|
|
8672
Uchino T, Takezawa T, Ikarashi Y.
Reconstruction of three-dimensional human skin model composed of dendritic cells, keratinocytes and fibroblasts utilizing a handy scaffold of collagen vitrigel membrane.
Toxicol In Vitro
2009
23:333-7
PubMed ID: 19121381
DOI: 10.1016/j.tiv.2008.12.003
|
|
3177
Tsukamoto, Hiroshi, Horiuchi, Takahiko, Kokuba, Hisashi, Nagae, Shonosuke, Nishizaka, Hiroaki, Sawabe, Takuya, Harashima, Shin-ichi, Himeji, Daisuke, Koyama, Takako, Otsuka, Junji, Mitoma, Hiroki, Kimoto, Yasutaka, Hashimura, Chinami, Kitano, Etsuko, Kitamura, Hajime, Furue, Masutaka, Harada, Mine
Molecular analysis of a novel hereditary C3 deficiency with systemic lupus erythematosus.
Biochem Biophys Res Commun
2005
330:298-304
PubMed ID: 15781264
DOI: 10.1016/j.bbrc.2005.02.159
|
|
6269
Kawashima H, Li YF, Watanabe N, Hirose J, Hirose M, Miyasaka M.
Identification and characterization of ligands for L-selectin in the kidney. I. Versican, a large chondroitin sulfate proteoglycan, is a ligand for L-selectin.
Int Immunol
1999
11(3):393-405
PubMed ID: 10221651
DOI: 10.1093/intimm/11.3.393
|
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