Cell No. : Cell Name
RCB0112 : NRK49F
update : 2023/06/30
|
| Comment | Contact-inhibited semi-normal cell line |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Ohno, Tadao
|
| Originator |
Delarco, J. E.
|
| Year of deposit |
1986
|
| Animal |
_rat
< Mammals
|
| Genus |
Rattus
|
| Species |
norvegicus
|
| Age at sampling |
adult
|
| Tissue |
kidney
|
| Classification |
semi-normal
|
|
Lifespan |
infinite
|
| Morphology |
fibroblast-like
|
| Cellosaurus(Expasy) |
CVCL_2144
|
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| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
| Culture medium |
|
MEM + 5% CS
|
| Antibiotics |
|
Free
|
|
Passage method |
|
0.25% Trypsin
|
Culture information
|
Passage ratio |
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1 : 8 split
|
| SC frequency |
|
Subculture : once/week, Medium Renewal : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
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| Mycoplasma |
|
(-)
|
| Animal PCR |
|
OK
|
| Isozyme |
|
LD, NP
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| Chromosome mode |
|
38-42(50) : 38(1),39(2),40(1),41(45),42(1)
|
| Images |
deposit info | lot info |
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|
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| Reference information |
Reference |
4
|
| User's Publication |
13
|
| User's Publication |
|
15439
Shams R, Matsukawa A, Ochi Y, Ito Y, Miyatake H.
In Silico and In Cell Hybrid Selection of Nonrapalog Ligands to Allosterically Inhibit the Kinase Activity of mTORC1
J Med Chem
2022
Jun 30
PubMed ID: 34191518
DOI: 10.1021/acs.jmedchem.1c00536
|
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13334
Yamamoto A, Morioki H, Nakae T, Miyake Y, Harada T, Noda S, Mitsuoka S, Matsumoto K, Tomimatsu M, Kanemoto S, Tanaka S, Maeda M, Conway SJ, Imaizumi K, Fujio Y, Obana M.
Transcription factor old astrocyte specifically induced substance is a novel regulator of kidney fibrosis.
FASEB J
2021
PubMed ID: 33150680
DOI: 10.1096/fj.202001820R
|
|
20241
Miyake Y, Obana M, Nakae T, Yamamoto A, Tanaka S, Maeda M, Okada Y, Fujio Y.
PKNOX2 regulates myofibroblast functions and tubular cell survival during kidney fibrosis.
Biochem Biophys Res Commun
2021
571:88-95
PubMed ID: 34311199
DOI: 10.1016/j.bbrc.2021.07.067
|
|
6958
Yan Q, Ina K, Chiba S1, Wei H, Tatsukawa S, Fujikura Y.
The signal pathway for the repressive effect of dipyridamole on myofibroblast transdifferentiation.
J Cell Mol Med
2019
23(2):1608-1612
PubMed ID: 30451359
DOI: 10.1111/jcmm.14006
|
|
12256
Toshihiko Tsutsumi, Syougo Yamakawa, Akira Ishihara, Aimi Yamamoto, Tamotsu Tanaka, Akira Tokumura
Reduced kidney levels of lysophosphatidic acids in rats after chronic administration of aristolochic acid: Its possible protective role in renal fibrosis
Toxicol Rep
2015
2:121-129
PubMed ID: 28962344
DOI: 10.1016/j.toxrep.2015.02.012
|
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15254
Yano R, Golbar HM, Izawa T, Sawamoto O, Kuwamura M, Yamate J.
Participation of bone morphogenetic protein (BMP)-6 and osteopontin in cisplatin (CDDP)-induced rat renal fibrosis
Exp Toxicol Pathol
2015
67(2):99-107
PubMed ID: 25467747
DOI: 10.1016/j.etp.2014.10.002
|
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15703
Jeonghwa Kang, Makoto Sakuragi, Aya Shibata, Hiroshi Abe, Takashi Kitajima, Seiichi Tada, Masayoshi Mizutani, Hitoshi Ohmori, Hirohito Ayame, Tae Il Son, Toshiro Aigaki, Yoshihiro Ito
Immobilization of epidermal growth factor on titanium and stainless steel surfaces via dopamine treatment
Materials Science and Engineering: C
2012
32:2552-2561
DOI: 10.1016/j.msec.2012.07.039
|
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16145
Nishi H, Inagi R, Kawada N, Yoshizato K, Mimura I, Fujita T, Nangaku M.
Cytoglobin, a novel member of the globin family, protects kidney fibroblasts against oxidative stress under ischemic conditions
Am J Pathol9
2011
178(1):128-3
PubMed ID: 21224051
DOI: 10.1016/j.ajpath.2010.11.011
|
|
8150
Mimura I, Nangaku M, Nishi H, Inagi R, Tanaka T, Fujita T.
Cytoglobin, a novel globin, plays an antifibrotic role in the kidney.
Am. J. Physiol. Renal Physiol.
2010
299:F1120-33
PubMed ID: 20719976
DOI: 10.1152/ajprenal.00145.2010
|
|
4734
Ishikawa E1, Tsuboi K, Takano S, Uchimura E, Nose T, Ohno T.
Intratumoral injection of IL-2-activated NK cells enhances the antitumor effect of intradermally injected paraformaldehyde-fixed tumor vaccine in a rat intracranial brain tumor model.
Cancer Sci
2004
95(1):98-103
PubMed ID: 14720334
DOI: 10.1111/j.1349-7006.2004.tb03177.x
|
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1933
Han K, Hashimoto N, Ikeda Y, Shiratori Y, Kato H, Toda G, Komatsu Y, Yamada H, Tanaka A, Kurokawa K
Occurrence of antibody against rat hepatic sinusoidal endothelial cells in sera of patients with autoimmune hepatitis.
Dig Dis Sci
1995
40:1213-20
PubMed ID: 7781435
DOI: 10.1007/BF02065526
|
|
17924
Kurimoto M, Endo S, Arai K, Horie Y, Nogami K, Takaku A.
TM-1 cells from an established human malignant glioma cell line produce PDGF, TGF-alpha, and TGF-beta which cooperatively play a stimulatory role for an autocrine growth promotion
J Neurooncol
1994
22(1):33-44
PubMed ID: 7714549
DOI: 10.1007/BF01058353
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|
17216
Yoshikatsu Ogawa, Yoshimitsu Yamazaki, Hiroaki Okuno
Stereoselectivity in the hydrolysis of synthetic esters by cultured cancer cells and normal tissue extracts of rat
Bioorganic & Medicinal Chemistry Letters
1994
4:757-760
DOI: 10.1016/s0960-894x(01)80196-7
|
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