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RCB0098 : A549  update : 2018/07/06
CommentLung carcinoma, refer to ATCC CCL185
特性(日) ヒト肺癌細胞の一つで、1972年に樹立された。制ガン剤のスクリーニングに利用される。
寄託者特性(英)
寄託者特性(日)
使用条件a
使用条件(英)Basically, there is no restriction regarding academic use.
使用条件(日)学術研究における利用につきましては原則として使用制限はありません。
提供申込書類 依頼書C-0001.pdf   同意書(非営利)C-0003.pdf   同意書(営利)C-0003p.pdf  
提供同意書は、使用機関及び使用内容に応じて二種類あります。該当する提供同意書をご使用ください(詳細)。特許等の取得及び商業利用等は別途ご相談(cellbank.brc@riken.jp)を承ります。
細胞基本情報 寄託者 Abe, Shin-ichiro
樹立者 Giard, D. J.
寄託日 1986
動物種 human < Mammals
属名 Homo
種名 sapiens
性別 Male
採取組織 lung
病歴 adenocarcinoma
細胞分類 cancer
細胞寿命 infinite
細胞形態 epithelial-like
細胞培養情報
寄託時情報
ロット情報
培地・試薬情報 培地・試薬一覧はこちらをご覧ください。
培養形態 Adherent cells
培地 DMEM + 10% FBS
抗生物質 Free
継代方法 0.25% trypsin
継代密度 1 : 8 split
継代または培地交換頻度 Subculture : 2 times/week
培養最適温度 37 ℃
凍結培地 Medium + 10% DMSO
凍結方法 Slow freezing
マイコプラズマ (-)
個体識別検査 OK
アイソザイム検査 LD, NP, G6PD
染色体検査 59-64(50) : 59(3),60(2),61(8),62(21),63(14),64(2)
画像情報
寄託時情報
ロット情報
文献情報 Reference(英) 0件
Reference(日) 0件
利用者成果(英) 31件
利用者成果(日) 0件

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Yamashita, Satoko, Katayama, Hideki, Saitoh, Hiroyuki, Akao, Tetsuyuki, Park, Yu Shin, Mizuki, Eiichi, Ohba, Michio, Ito, Akio  Typical Three-Domain Cry Proteins of Bacillus thuringiensis Strain A1462 Exhibit Cytocidal Activity on Limited Human Cancer Cells.  J Biochem  2005  138:663-72 
Okajima, Shuhei, Yamaguchi, Takeo, Sakai, Yasuyuki, Nakao, Shin-ichi  Regulation of cell adhesion using a signal-responsive membrane substrate.  Biotechnol Bioeng  2005  91:237-43 
Sato, Masamitsu, Takahashi, Kenji, Nagayama, Kazuhiro, Arai, Yasuhito, Ito, Nobue, Okada, Mitsumasa, Minna, John D, Yokota, Jun, Kohno, Takashi  Identification of chromosome arm 9p as the most frequent target of homozygous deletions in lung cancer.  Genes Chromosomes Cancer  2005  44:405-14 
Kajiura K, Masuda K, Naruto T, Kohmoto T, Watabnabe M, Tsuboi M, Takizawa H, Kondo K, Tangoku A, Imoto I.  Frequent silencing of the candidate tumor suppressor TRIM58 by promoter methylation in early-stage lung adenocarcinoma.  Oncotarget  2016  8:2890-2905  PubMed ID: 27926516
Morita S, Noguchi H, Horii T, Nakabayashi K, Kimura M, Okamura K, Sakai A, Nakashima H, Hata K, Nakashima K, Hatada I.  Targeted DNA demethylation in vivo using dCas9-peptide repeat and scFv-TET1 catalytic domain fusions.  Nat. Biotechnol.  2016  34:1060-1065  PubMed ID: 27571369
Miura T, Kakehashi H, Shinkai Y, Egara Y, Hirose R, Cho AK, Kumagai Y.  GSH-mediated S-transarylation of a quinone glyceraldehyde-3-phosphate dehydrogenase conjugate.  Chem. Res. Toxicol.  2011  24:1836-44  PubMed ID: 21827172
Hoshino A, Ishii G, Ito T, Aoyagi K, Ohtaki Y, Nagai K, Sasaki H, Ochiai A.  Podoplanin-positive fibroblasts enhance lung adenocarcinoma tumor formation: podoplanin in fibroblast functions for tumor progression.  Cancer Res.  2011  71:4769-79  PubMed ID: 21610106
Tahara K, Sakai T, Yamamoto H, Takeuchi H, Hirashima N, Kawashima Y.  Improvements in transfection efficiency with chitosan modified poly(DL-lactide-co-glycolide) nanospheres prepared by the emulsion solvent diffusion method, for gene delivery.  Chem. Pharm. Bull.  2011  59:298-301  PubMed ID: 21372409
Okamoto H.  Hepatitis E virus cell culture models.  Virus Res.  2011  161:65-77  PubMed ID: 21316402
Tada E, Toyomura K, Nakamura H, Sasaki H, Saito T, Kaneko M, Okuma Y, Murayama T.  Activation of ceramidase and ceramide kinase by vanadate via a tyrosine kinase-mediated pathway.  J. Pharmacol. Sci.  2010  114:420-32  PubMed ID: 21127389
Ishigaki Y, Nakamura Y, Takehara T, Nemoto N, Kurihara T, Koga H, Nakagawa H, Takegami T, Tomosugi N, Miyazawa S, Kuwabata S.  Ionic liquid enables simple and rapid sample preparation of human culturing cells for scanning electron microscope analysis.  Microsc. Res. Tech.  2010  74:415-20  PubMed ID: 20830703
Haruki T, Shomori K, Hamamoto Y, Taniguchi Y, Nakamura H, Ito H.  Geminin expression in small lung adenocarcinomas: implication of prognostic significance.  Lung Cancer  2010  71:356-62  PubMed ID: 20650542
Tsuji S, Tsuura Y, Morohoshi T, Shinohara T, Oshita F, Yamada K, Kameda Y, Ohtsu T, Nakamura Y, Miyagi Y.  Secretion of intelectin-1 from malignant pleural mesothelioma into pleural effusion.  Br. J. Cancer  2010  103:517-23  PubMed ID: 20628387
Horie M, Nishio K, Fujita K, Kato H, Endoh S, Suzuki M, Nakamura A, Miyauchi A, Kinugasa S, Yamamoto K, Iwahashi H, Murayama H, Niki E, Yoshida Y.  Cellular responses by stable and uniform ultrafine titanium dioxide particles in culture-medium dispersions when secondary particle size was 100 nm or less.  Toxicol In Vitro  2010  24:1629-38  PubMed ID: 20541599
Li F, Awale S, Tezuka Y, Kadota S.  Cytotoxic constituents of propolis from Myanmar and their structure-activity relationship.  Biol. Pharm. Bull.  2009  32:2075-8  PubMed ID: 19952433
Matsuzawa Y, Kiuchi Y, Toyomura K, Matsumoto I, Nakamura H, Fujino H, Murayama T, Kawashima T.  Activation of cytosolic phospholipase A2alpha by epidermal growth factor (EGF) and phorbol ester in HeLa cells: different effects of inhibitors for EGF receptor, protein kinase C, Src, and C-Raf.  J. Pharmacol. Sci.  2009  111:182-92  PubMed ID: 19783865
Awale S, Miyamoto T, Linn TZ, Li F, Win NN, Tezuka Y, Esumi H, Kadota S.  Cytotoxic constituents of Soymida febrifuga from Myanmar.  J. Nat. Prod.  2009  72:1631-6  PubMed ID: 19689125
Tahara K, Sakai T, Yamamoto H, Takeuchi H, Hirashima N, Kawashima Y.  Improved cellular uptake of chitosan-modified PLGA nanospheres by A549 cells.  Int J Pharm  2009  382:198-204  PubMed ID: 19646519
Fukasawa C, Ishiwada N, Ogita J, Hishiki H, Kohno Y.  The effects of disodium cromoglycate on enhanced adherence of Haemophilus influenzae to A549 cells infected with respiratory syncytial virus.  Pediatr. Res.  2009  66:168-73  PubMed ID: 19390482
Iwakiri S, Mino N, Takahashi T, Sonobe M, Nagai S, Okubo K, Wada H, Date H, Miyahara R.  Higher expression of chemokine receptor CXCR7 is linked to early and metastatic recurrence in pathological stage I nonsmall cell lung cancer.  Cancer  2009  115:2580-93  PubMed ID: 19309748
Kawanami Y, Morimoto Y, Kim H, Nakamura T, Machida K, Kido T, Asonuma E, Yatera K, Yoshii C, Kido M.  Calcitonin gene-related peptide stimulates proliferation of alveolar epithelial cells.  Respir. Res.  2009  10:8  PubMed ID: 19192276
Yumi Motoyama,a Kanae Bekki,a Sang Woon Chung,a Ning Tang,b Takayuki Kameda,b. Akira Toriba,b Keiko Taguchi, Kazuichi Hayakawa  Oxidative Stress More Strongly Induced by ortho- Than para-quinoid Polycyclic Aromatic Hydrocarbons in A549 Cells  RCB0098  2009  55:845-850 
Ozawa T, Miyata M, Nishimura M, Ando T, Ouyang Y, Ohba T, Shimokawa N, Ohnuma Y, Katoh R, Ogawa H, Nakao A.  Transforming growth factor-beta activity in commercially available pasteurized cow milk provides protection against inflammation in mice.  J. Nutr.  2008  139:69-75  PubMed ID: 19056655
Tanaka T, Takahashi M, Kusano E, Okamoto H.  Development and evaluation of an efficient cell-culture system for Hepatitis E virus.  J. Gen. Virol.  2007  88:903-11  PubMed ID: 17325363
Morita M, Sato T, Nomura M, Sakamoto Y, Inoue Y, Tanaka R, Ito S, Kurosawa K, Yamaguchi K, Sugiura Y, Takizaki H, Yamashita Y, Katakura R, Sato I, Kawai M, Okada Y, Watanabe H, Kondoh G, Matsumoto S, Kishimoto A, Obata M, Matsumoto M, Fukuhara T, Motohashi H, Suematsu M, Komatsu M, Nakayama KI, Watanabe T, Soga T, Shima H, Maemondo M, Tanuma N.  PKM1 Confers Metabolic Advantages and Promotes Cell-Autonomous Tumor Cell Growth.  Cancer Cell  2018  33:355-367.e7  PubMed ID: 29533781
Nagashima S, Kobayashi T, Tanaka T, Tanggis , Jirintai S, Takahashi M, Nishizawa T, Okamoto H.  Analysis of adaptive mutations selected during the consecutive passages of hepatitis E virus produced from an infectious cDNA clone.  Virus Res.  2016  223:170-80  PubMed ID: 27485920
Kobayashi A, Tengku Ahmad TAF, Autsavapromporn N, Oikawa M, Homma-Takeda S, Furusawa Y, Wang J, Konishi T.  Enhanced DNA double-strand break repair of microbeam targeted A549 lung carcinoma cells by adjacent WI38 normal lung fibroblast cells via bi-directional signaling.  Mutat. Res.  2017  803-805:1-8  PubMed ID: 28689138
Horibe S, Tanahashi T, Kawauchi S, Murakami Y, Rikitake Y.  Mechanism of recipient cell-dependent differences in exosome uptake.  BMC Cancer  2018  18:47  PubMed ID: 29306323
Tahara K, Kobayashi M, Yoshida S, Onodera R, Inoue N, Takeuchi H.  Effects of cationic liposomes with stearylamine against virus infection.  Int J Pharm  2018  543:311-317  PubMed ID: 29625169
  Biocompatible CdSe/ZnS quantum dot micelles for long-term cell imaging without alteration to the native structure of the blood plasma protein human serum albumin        PubMed ID:
  METHOD FOR INDUCING CELL REPROGRAMMING, AND METHOD FOR PRODUCING PLURIPOTENT CELLS        PubMed ID:

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