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RCB0027 : K562  update : 2018/07/31
CommentChronic myelogenous leukemia, sensitive to NK cell. Differentiate to erythroid cells .
Comment from the depositor
Terms and conditionsBasically, there is no restriction regarding academic use.
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.
Basic information Depositor Kanazawa, Satoshi
Originator Lozzio, C.B. & Lozzio, B.B.
Year of deposit 1986
Animal human < Mammals
Genus Homo
Species sapiens
Gender Female
Age at sampling 53 years
Tissue pleural effusion
Case history chronic myelogenous leukemia
Classification cancer
Lifespan infinite
Morphology lymphoblast-like
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives HamF12 + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR (human) OK
Virus (HIV) (-)
Virus (HTLV-1) (-)
Isozyme LD, NP
Chromosome mode 59-68(50) : /63(5),64(7),65(6),66(8),67(16),68(5)
deposit info
lot info
Reference information Reference 5
User's Publication 27

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4602  Lozzio CB, Lozzio BB.  Human chronic myelogenous leukemia cell-line with positive Philadelphia chromosome.  Blood  1975  45(3):321-34  PubMed ID: 163658
4603  Lozzio BB, Lozzio CB.  Properties and usefulness of the original K-562 human myelogenous leukemia cell line.  Leuk Res  1979  3(6):363-70  PubMed ID: 95026
5042  Haruta Y, Seon BK.  Distinct human leukemia-associated cell surface glycoprotein GP160 defined by monoclonal antibody SN6.  Proc Natl Acad Sci U S A  1986  ;83(20):7898-902  PubMed ID: 3464004
969  Rutherford TR, Clegg JB, Weatherall DJ.  K562 human leukaemic cells synthesise embryonic haemoglobin in response to haemin.  Nature  1979  280(5718):164-5  PubMed ID: 95354
970  Andersson LC, Jokinen M, Gahmberg CG.  Induction of erythroid differentiation in the human leukaemia cell line K562.  Nature  1979  278(5702):364-5  PubMed ID: 570644

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User's Publication
2550  Tamura, H O, Taniguchi K, Hayashi E, Hiyoshi Y, Nagai F  Expression profiling of sulfotransferases in human cell lines derived from extra-hepatic tissues.  Biol Pharm Bull  2001  24:1258-62  PubMed ID: 11725959
2549  Nagai, Fusako, Hiyoshi, Yasuko, Sugimachi, Kumiko, Tamura, Hiro-Omi  Cytochrome P450 (CYP) expression in human myeloblastic and lymphoid cell lines.  Biol Pharm Bull  2002  25:383-5  PubMed ID: 11913539
3407  Ito, Mikiko, Sakai, Yuko, Furumoto, Mari, Segawa, Hiroko, Haito, Sakiko, Yamanaka, Setsuko, Nakamura, Rie, Kuwahata, Masashi, Miyamoto, Ken-ichi  Vitamin D and phosphate regulate fibroblast growth factor-23 in K-562 cells.  Am J Physiol Endocrinol Metab  2005  288:E1101-9  PubMed ID: 15671080
8795  Engelhardt P, Merilainen J, Zhao F, Uchiyama S, Fukui K, Lehto VP.  Whole-mount immunoelectron tomography of chromosomes and cells.  Methods Mol. Biol.  2007  369:387-405  PubMed ID: 17656761
9051  Tominaga K, Kagata T, Johmura Y, Hishida T, Nishizuka M, Imagawa M.  SLC39A14, a LZT protein, is induced in adipogenesis and transports zinc.  FEBS J.  2005  272:1590-9  PubMed ID: 15794747
9546  Nakamura A, Monzen S, Takasugi Y, Wojcik A, Mariya Y.  Application of cell sorting for enhancing the performance of the cytokinesis-block micronucleus assay.  J. Radiat. Res.  2016  57:121-6  PubMed ID: 26826197
11093  Abdelmoez MN, Iida K, Oguchi Y, Nishikii H, Yokokawa R, Kotera H, Uemura S, Santiago JG, Shintaku H.  SINC-seq: correlation of transient gene expressions between nucleus and cytoplasm reflects single-cell physiology.  Genome Biol.  2018    PubMed ID: 29871653
4270  Ichii M, Oritani K, Murase M, Komatsu K, Yamazaki M, Kyoden R, Kito N, Nozaki Y, Saito M, Iwamura H, Kanakura Y.  Molecular targeting of inosine-5'-monophosphate dehydrogenase by FF-10501 promotes erythropoiesis via ROS/MAPK pathway.  Leuk. Lymphoma  2018    PubMed ID: 28730859
4378  Miyauchi M, Koya J, Arai S, Yamazaki S, Honda A, Kataoka K, Yoshimi A, Taoka K, Kumano K, Kurokawa M.  ADAM8 Is an Antigen of Tyrosine Kinase Inhibitor-Resistant Chronic Myeloid Leukemia Cells Identified by Patient-Derived Induced Pluripotent Stem Cells.  Stem Cell Reports  2018    PubMed ID: 29429960
4885  Sonoyama J, Matsumura I, Ezoe S, Satoh Y, Zhang X, Kataoka Y, Takai E, Mizuki M, Machii T, Wakao H, Kanakura Y.  Functional cooperation among Ras, STAT5, and phosphatidylinositol 3-kinase is required for full oncogenic activities of BCR/ABL in K562 cells.  J Biol Chem  2002  277(10):8076-82  PubMed ID: 11779872
5396  Hosomi O, Misawa Y, Takeya A, Matahira Y, Sugahara K, Kubohara Y, Yamakura F, Kudo S.  Novel oligosaccharide has suppressive activity against human leukemia cell proliferation.  Glycoconj J  2009  26(2):189-98  PubMed ID: 18726690
1332  Shionoya S.  Etoposide inhibition of methylaminoisobutyric acid transport and its recovery in K562 cells.  Leuk Res  1991  15(2-3):137-41  PubMed ID: 2016905
2381  Suzuki K, Tsujitani S, Konishi I, Yamaguchi Y, Hirooka Y, Kaibara N.  Expression of MAGE genes and survival in patients with hepatocellular carcinoma.  Int J Oncol  1999  15(6):1227-32  PubMed ID: 10568832
2880  Okada M, Adachi S, Imai T, Watanabe K, Toyokuni SY, Ueno M, Zervos AS, Kroemer G, Nakahata T.  A novel mechanism for imatinib mesylate-induced cell death of BCR-ABL-positive human leukemic cells: caspase-independent, necrosis-like programmed cell death mediated by serine protease activity.  Blood  2004  103(6):2299-307  PubMed ID: 14645012
2979  Horiguchi-Yamada J, Fukumi S, Saito S, Nakayama R, Iwase S, Yamada H.  DNA topoisomerase II inhibitor, etoposide, induces p21WAF1/CIP1 through down-regulation of c-Myc in K562 cells.  Anticancer Res  2002  22(6C):3827-32  PubMed ID: 12553001
2983  Kawano T, Horiguchi-Yamada J, Iwase S, Akiyama M, Furukawa Y, Kan Y, Yamada H.  Depsipeptide enhances imatinib mesylate-induced apoptosis of Bcr-Abl-positive cells and ectopic expression of cyclin D1, c-Myc or active MEK abrogates this effect.  Anticancer Res  2004  24(5A):2705-12  PubMed ID: 15517875
2984  Kawano T, Horiguchi-Yamada J, Saito S, Iwase S, Furukawa Y, Kano Y, Yamada H.  Ectopic cyclin D1 expression blocks STI571-induced erythroid differentiation of K562 cells.  Leuk Res  2004  28(6):623-9  PubMed ID: 15120940
2985  Kawano T, Horiguchi-Yamada J, Iwase S, Furukawa Y, Kano Y, Yamada H.  Inactivation of ERK accelerates erythroid differentiation of K562 cells induced by herbimycin A and STI571 while activation of MEK1 interferes with it.  Mol Cell Biochem  2004  58(1-2):25-33  PubMed ID: 15030167
2986  Fukumi S, Horiguchi-Yamada J, Iwase S, Ohno T, Yamada H.  Concentration-dependent variable effects of etoposide on the cell cycle of CML cells.  Anticancer Res  2000  20(5A):3105-10  PubMed ID: 11062729
2987  Akiyama M, Yamada O, Akita S, Urashima M, Horiguchi-Yamada J, Ohno T, Mizoguchi H, Eto Y, Yamada H.  Ectopic expression of c-myc fails to overcome downregulation of telomerase activity induced by herbimycin A, but ectopic hTERT expression overcomes it.  Leukemia  2000  14(7):1260-5  PubMed ID: 10914551
2988  Fukumi S, Horiguchi-Yamada J, Nakada S, Nagai M, Ohno T, Yamada H.  Differential responses of Bcl-2 family genes to etoposide in chronic myeloid leukemia K562 cells.  Mol Cell Biochem  2000  206(1-2):43-50  PubMed ID: 10839193
2989  Yamada H, Iwase S, Nagai M, Nemoto T, Sekikawa T, Takahara S, Nakada S, Furukawa Y, Horiguchi-Yamada J.  Herbimycin A down-regulates messages of cyclin D1 and c-myc during erythroid differentiation of K562 cells.  Int J Hematol  1996  65(1):31-40  PubMed ID: 8990623
2991  Yamada H, Horiguchi-Yamada J, Nagai M, Takahara S, Sekikawa T, Kawano T, Itoh K, Fukumi S, Iwase S.  Biological effects of a relatively low concentration of 1-beta-D-arabinofuranosylcytosine in K562 cells: alterations of the cell cycle, erythroid-differentiation, and apoptosis.  Mol Cell Biochem  1998  187(1-2):211-20  PubMed ID: 9788759
3118  Uchiyama S, Kobayashi S, Takata H, Ishihara T, Sone T, Matsunaga S, Fukui K.  Protein composition of human metaphase chromosomes analyzed by two-dimensional electrophoreses.  Cytogenet Genome Res  2004  107(1-2):49-54  PubMed ID: 15305056
3170  Harada H, Suzu S, Ito T, Okada S.  Selective expansion and engraftment of human CD16+ NK cells in NOD/SCID mice.  Eur J Immunol  2005  35(12):3599-609  PubMed ID: 16304638
3979  Matsumoto K, Akao Y, Kobayashi E, Ohguchi K, Ito T, Tanaka T, Iinuma M, Nozawa Y.  Induction of apoptosis by xanthones from mangosteen in human leukemia cell lines.  J Nat Prod  2003  66(8):1124-7  PubMed ID: 12932141
5654  Sadahira Y, Sugihara T, Fujiwara H, Nishimura H, Suetsugu Y, Takeshita M, Okamura S, Goto M.  WRN protein as a novel erythroblast immunohistochemical marker with applications for the diagnosis of Werner syndrome.  Virchows Arch  2015  466(3):343-50  PubMed ID: 25503078

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