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RCB0008 : V79  update : 2018/07/06
CommentChinese hamster lung fibroblast
Comment from the depositor
Terms and conditionsBasically, there is no restriction regarding academic use.
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.
Basic information Depositor Takahashi, Kazuhide
Originator Elkind, M. M.
Year of deposit 1985
Animal hamster < Mammals
Strain name Chinese
Gender Male
Tissue lung
Classification other
History RIKEN-I.Kaneko
Lifespan infinite
Morphology fibroblast-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives MEM + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP
Chromosome mode 19-25(50) : 19(2),21(1),22(41),23(5),25(1)
deposit info
lot info
Reference information Reference 3
User's Publication 5

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2204  Jones, N J, Cox R, Thacker J  Isolation and cross-sensitivity of X-ray-sensitive mutants of V79-4 hamster cells.  Mutat Res  1987  183:279-86  PubMed ID: 3106801
346  ELKIND, M M, SUTTON H  Radiation response of mammalian cells grown in culture. 1. Repair of X-ray damage in surviving Chinese hamster cells.  Radiat Res  1960  13:556-93  PubMed ID: 13726391
344  FORD, D K, YERGANIAN G  Observations on the chromosomes of Chinese hamster cells in tissue culture.  J Natl Cancer Inst  1958  21:393-425  PubMed ID: 13576097

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User's Publication
9549  Maeda K, Yasui H, Matsuura T, Yamamori T, Suzuki M, Nagane M, Nam JM, Inanami O, Shirato H.  Evaluation of the relative biological effectiveness of spot-scanning proton irradiation in vitro.  J. Radiat. Res.  2016  57:307-11  PubMed ID: 26838131
8046  Shirai T, Shimizu T, Ohtani K, Zen Y, Takaya M, Tsuchiya H.  Antibacterial iodine-supported titanium implants.  Acta Biomater  2010  7:1928-33  PubMed ID: 21115142
8296  Yoshiki Ando, Hiroshi Miyamoto, Iwao Noda, Nobuko Sakurai, Tomonori Akiyama, Yutaka Yonekura, Takafumi Shimazaki, Masaki Miyazaki, Masaaki Mawatari, Takao Hotokebuchi  Calcium phosphate coating containing silver shows high antibacterial activity and low cytotoxicity and inhibits bacterial adhesion  Materials Science and Engineering: C  2010  30:175-180 
10971    Antigenotoxic effect of Pleurotus cornucopiae extracts on the mutagenesis of Salmonella typhimurium TA 98 elicited by benzo[a]pyrene and oxidative DNA lesions in V 79 hamster lung cells  Japanese Journal of Veterinary Research  2005  52, 163-172 
2013  Kawamoto, Tetsuji, Ikeuchi, Yoshihiro, Hirano, Junko, Eikyu, Yoshiteru, Shimizu, Kazue, Torishima, Masaki, Bessho, Kiyoshi, and Yoneda, Fumio  Evalution of Differential Hypoxic Cytotoxicity and Electrochemical Studies of Nitro 5-Deazaflavins  Bioorganic & Medicinal Chemistry Letters  1995  5:2115-2118 

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